caa a22 4500 445321172 CHVBK 20180317142704.0 cr unu---uuuuu 170323e20110201xx s 000 0 eng 10.1007/s10534-010-9382-5 doi (NATIONALLICENCE)springer-10.1007/s10534-010-9382-5 Influence of zinc and zinc chelator on HT-29 colorectal cell line [Elektronische Daten] [K. Gurusamy, N. Farooqui, M. Loizidou, S. Dijk, J. Taanman, S. Whiting, M. Farquharson, B. Fuller, B. Davidson] Trace elements are involved in many key pathways involving cell cycle control. The influence of zinc and zinc chelator (TPEN) on transcription levels of the main zinc transporters (ZnT1 and ZIP1) in the HT-29 colorectal cell line has not been reported. Proliferation of HT-29 cells was measured using the methylene blue assay after exposure to zinc (two concentrations), TPEN (two concentrations), or a combination of zinc and TPEN (simultaneously and sequentially) for 4h, 8h, and 24h. The transcription levels of ZnT1, ZIP1, vascular endothelial growth factor (VEGF), and caspase-3 were determined using reverse transcriptase real-time polymerase chain reaction (RT-PCR) after exposure of cells to zinc and TPEN. The zinc content in the substrate (medium used for culture) was determined using atomic absorption spectrometry. TPEN decreased cellular proliferation causing complete cell death by 8h. Zinc had a protective effect against short periods of exposure to TPEN. There was no correlation between the transcripts of main zinc transporters and the zinc content in the substrate. The zinc content in the substrate remained constant after varying periods of cell culture. TPEN decreased the transcript levels of caspase-3 and VEGF, which are surrogate markers for apoptosis and angiogenesis. Zinc chelation of HT-29 cells causes cell death. Zinc appears to be protective for short periods of exposure to TPEN but has no protective effect on prolonged exposure. HT-29 cells are not able to counteract the effect of intracellular chelation of zinc by altering zinc transport. Further research into the mechanisms of these findings is necessary and may lead to novel therapeutic options. Springer Science+Business Media, LLC., 2010 Zinc nationallicence Zinc chelator nationallicence Angiogenesis nationallicence Apoptosis nationallicence HT-29 nationallicence Gurusamy K. University Department of Surgery, Royal Free Campus, UCL Medical School, London, UK aut Farooqui N. University Department of Surgery, Royal Free Campus, UCL Medical School, London, UK aut Loizidou M. University Department of Surgery, Royal Free Campus, UCL Medical School, London, UK aut Dijk S. University Department of Surgery, Royal Free Campus, UCL Medical School, London, UK aut Taanman J. Department of Clinical Neurosciences, Royal Free Campus, UCL Medical School, London, UK aut Whiting S. Department of Clinical Biochemistry, Royal Free Hospital, London, UK aut Farquharson M. Department of Medical Physics and Applied Radiation Sciences, McMaster University, 1280 Main Street West, L8S 4K1, Hamilton, ON, Canada aut Fuller B. University Department of Surgery, Royal Free Campus, UCL Medical School, London, UK aut Davidson B. University Department of Surgery, Royal Free Campus, UCL Medical School, London, UK aut BioMetals Springer Netherlands 24/1(2011-02-01), 143-151 0966-0844 24:1<143 2011 24 10534 https://doi.org/10.1007/s10534-010-9382-5 text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1007/s10534-010-9382-5 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Gurusamy K. University Department of Surgery, Royal Free Campus, UCL Medical School, London, UK aut NATIONALLICENCE 700 1- Farooqui N. University Department of Surgery, Royal Free Campus, UCL Medical School, London, UK aut NATIONALLICENCE 700 1- Loizidou M. University Department of Surgery, Royal Free Campus, UCL Medical School, London, UK aut NATIONALLICENCE 700 1- Dijk S. University Department of Surgery, Royal Free Campus, UCL Medical School, London, UK aut NATIONALLICENCE 700 1- Taanman J. Department of Clinical Neurosciences, Royal Free Campus, UCL Medical School, London, UK aut NATIONALLICENCE 700 1- Whiting S. Department of Clinical Biochemistry, Royal Free Hospital, London, UK aut NATIONALLICENCE 700 1- Farquharson M. Department of Medical Physics and Applied Radiation Sciences, McMaster University, 1280 Main Street West, L8S 4K1, Hamilton, ON, Canada aut NATIONALLICENCE 700 1- Fuller B. University Department of Surgery, Royal Free Campus, UCL Medical School, London, UK aut NATIONALLICENCE 700 1- Davidson B. University Department of Surgery, Royal Free Campus, UCL Medical School, London, UK aut NATIONALLICENCE 773 0- BioMetals Springer Netherlands 24/1(2011-02-01), 143-151 0966-0844 24:1<143 2011 24 10534 Metadata rights reserved Springer special CC-BY-NC licence nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer