caa a22 4500 445321180 CHVBK 20180317142704.0 cr unu---uuuuu 170323e20110201xx s 000 0 eng 10.1007/s10534-010-9381-6 doi (NATIONALLICENCE)springer-10.1007/s10534-010-9381-6 Antibacterial effect of silver nanoparticles on Staphylococcus aureus [Elektronische Daten] [Wen-Ru Li, Xiao-Bao Xie, Qing-Shan Shi, Shun-Shan Duan, You-Sheng Ouyang, Yi-Ben Chen] The antibacterial activity and mechanism of silver nanoparticles (Ag-NPs) on Staphylococcus aureus ATCC 6538P were investigated in this study. The experiment results showed the minimum bactericidal concentration (MBC) of Ag-NPs to S. aureus was 20μg/ml. Moreover, when bacteria cells were exposed to 50μg/ml Ag-NPs for 6h, the cell DNA was condensed to a tension state and could have lost their replicating abilities. When S. aureus cells were exposed to 50μg/ml Ag-NPs for 12h, the cell wall was breakdown, resulting in the release of the cellular contents into the surrounding environments, and finally became collapsed. And Ag-NPs could reduce the enzymatic activity of respiratory chain dehydrogenase. Furthermore, the proteomic analysis showed that the expression abundance of some proteins was changed in the treated bacterial cell with Ag-NPs, formate acetyltransferase increased 5.3-fold in expression abundance, aerobic glycerol-3-phosphate dehydrogenase decreased 6.5-fold, ABC transporter ATP-binding protein decreased 6.2-fold, and recombinase A protein decreased 4.9-fold. Springer Science+Business Media, LLC., 2010 Silver nanoparticles (Ag-NPs) nationallicence Staphylococcus aureus nationallicence Antibacterial effect nationallicence Li Wen-Ru Guangdong Provincial Key Laboratory of Microbial Culture Collection and Application, Guangdong Open Laboratory of Applied Microbiology, Guangdong Institute of Microbiology, 510070, Guangzhou, China aut Xie Xiao-Bao Guangdong Provincial Key Laboratory of Microbial Culture Collection and Application, Guangdong Open Laboratory of Applied Microbiology, Guangdong Institute of Microbiology, 510070, Guangzhou, China aut Shi Qing-Shan Guangdong Provincial Key Laboratory of Microbial Culture Collection and Application, Guangdong Open Laboratory of Applied Microbiology, Guangdong Institute of Microbiology, 510070, Guangzhou, China aut Duan Shun-Shan Research Center of Hydrobiology, Jinan University, 510632, Guangzhou, China aut Ouyang You-Sheng Guangdong Provincial Key Laboratory of Microbial Culture Collection and Application, Guangdong Open Laboratory of Applied Microbiology, Guangdong Institute of Microbiology, 510070, Guangzhou, China aut Chen Yi-Ben Guangdong Provincial Key Laboratory of Microbial Culture Collection and Application, Guangdong Open Laboratory of Applied Microbiology, Guangdong Institute of Microbiology, 510070, Guangzhou, China aut BioMetals Springer Netherlands 24/1(2011-02-01), 135-141 0966-0844 24:1<135 2011 24 10534 https://doi.org/10.1007/s10534-010-9381-6 text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1007/s10534-010-9381-6 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Li Wen-Ru Guangdong Provincial Key Laboratory of Microbial Culture Collection and Application, Guangdong Open Laboratory of Applied Microbiology, Guangdong Institute of Microbiology, 510070, Guangzhou, China aut NATIONALLICENCE 700 1- Xie Xiao-Bao Guangdong Provincial Key Laboratory of Microbial Culture Collection and Application, Guangdong Open Laboratory of Applied Microbiology, Guangdong Institute of Microbiology, 510070, Guangzhou, China aut NATIONALLICENCE 700 1- Shi Qing-Shan Guangdong Provincial Key Laboratory of Microbial Culture Collection and Application, Guangdong Open Laboratory of Applied Microbiology, Guangdong Institute of Microbiology, 510070, Guangzhou, China aut NATIONALLICENCE 700 1- Duan Shun-Shan Research Center of Hydrobiology, Jinan University, 510632, Guangzhou, China aut NATIONALLICENCE 700 1- Ouyang You-Sheng Guangdong Provincial Key Laboratory of Microbial Culture Collection and Application, Guangdong Open Laboratory of Applied Microbiology, Guangdong Institute of Microbiology, 510070, Guangzhou, China aut NATIONALLICENCE 700 1- Chen Yi-Ben Guangdong Provincial Key Laboratory of Microbial Culture Collection and Application, Guangdong Open Laboratory of Applied Microbiology, Guangdong Institute of Microbiology, 510070, Guangzhou, China aut NATIONALLICENCE 773 0- BioMetals Springer Netherlands 24/1(2011-02-01), 135-141 0966-0844 24:1<135 2011 24 10534 Metadata rights reserved Springer special CC-BY-NC licence nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer