caa a22 4500 445321202 CHVBK 20180317142704.0 cr unu---uuuuu 170323e20111201xx s 000 0 eng 10.1007/s10534-011-9465-y doi (NATIONALLICENCE)springer-10.1007/s10534-011-9465-y Comparative antimicrobial activity and mechanism of action of bovine lactoferricin-derived synthetic peptides [Elektronische Daten] [Yifan Liu, Feifei Han, Yonggang Xie, Yizhen Wang] Lactoferricin B (LfcinB), a 25 residue peptide derived from the N-terminal of bovine lactoferrin (bLF), causes depolarization of the cytoplasmic membrane in susceptible bacteria. Its mechanism of action, however, still needs to be elucidated. In the present study, synthetic LfcinB (without a disulfide bridge) and LfcinB (C-C; with a disulfide bridge) as well as three derivatives with 15-, 11- and 9-residue peptides were prepared to investigate their antimicrobial nature and mechanisms. The antimicrobial properties were measured via minimum inhibitory concentration (MIC) determinations, killing kinetics assays and synergy testing, and hemolytic activities were assessed by hemoglobin release. Finally, the morphology of peptide-treated bacteria was determined by atomic force microscopy (AFM). We found that there was no difference in MICs between LfcinB and LfcinB (C-C). Among the derivatives, only LfcinB15 maintained nearly the same level as LfcinB, in the MIC range of 16-128μg/ml, and the MICs of LfcinB11 (64-256μg/ml) were 4 times more than LfcinB, while LfcinB9 exhibited the lowest antimicrobial activity. When treated at MIC for 1h, many blebs were formed and holes of various sizes appeared on the cell surface, but the cell still maintained its integrity. This suggested that LfcinB had a major permeability effect on the cytoplasmic membrane of both Gram-positive and Gram-negative bacteria, which also indicated it may be a possible intracellular target. Among the tested antibiotics, aureomycin increased the bactericidal activity of LfcinB against E. coli, S. aureus and P. aeruginosa, but neomycin did not have such an effect. We also found that the combination of cecropin A and LfcinB had synergistic effects against E. coli. Springer Science+Business Media, LLC., 2011 Bovine lactoferricin nationallicence Antimicrobial properties nationallicence Hemolytic activity nationallicence Mechanism of action nationallicence Synergistic effects nationallicence Liu Yifan National Engineering Laboratory of Biological Feed Safety and Pollution Prevention and Control, The Key Laboratory of Molecular Animal Nutrition of Ministry of Education, The Key Laboratory of Feed and Animal Nutrition of Zhejiang Province, Institute of Feed Science, Zhejiang University, 310029, Hangzhou, People's Republic of China aut Han Feifei National Engineering Laboratory of Biological Feed Safety and Pollution Prevention and Control, The Key Laboratory of Molecular Animal Nutrition of Ministry of Education, The Key Laboratory of Feed and Animal Nutrition of Zhejiang Province, Institute of Feed Science, Zhejiang University, 310029, Hangzhou, People's Republic of China aut Xie Yonggang National Engineering Laboratory of Biological Feed Safety and Pollution Prevention and Control, The Key Laboratory of Molecular Animal Nutrition of Ministry of Education, The Key Laboratory of Feed and Animal Nutrition of Zhejiang Province, Institute of Feed Science, Zhejiang University, 310029, Hangzhou, People's Republic of China aut Wang Yizhen National Engineering Laboratory of Biological Feed Safety and Pollution Prevention and Control, The Key Laboratory of Molecular Animal Nutrition of Ministry of Education, The Key Laboratory of Feed and Animal Nutrition of Zhejiang Province, Institute of Feed Science, Zhejiang University, 310029, Hangzhou, People's Republic of China aut BioMetals Springer Netherlands 24/6(2011-12-01), 1069-1078 0966-0844 24:6<1069 2011 24 10534 https://doi.org/10.1007/s10534-011-9465-y text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1007/s10534-011-9465-y text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Liu Yifan National Engineering Laboratory of Biological Feed Safety and Pollution Prevention and Control, The Key Laboratory of Molecular Animal Nutrition of Ministry of Education, The Key Laboratory of Feed and Animal Nutrition of Zhejiang Province, Institute of Feed Science, Zhejiang University, 310029, Hangzhou, People's Republic of China aut NATIONALLICENCE 700 1- Han Feifei National Engineering Laboratory of Biological Feed Safety and Pollution Prevention and Control, The Key Laboratory of Molecular Animal Nutrition of Ministry of Education, The Key Laboratory of Feed and Animal Nutrition of Zhejiang Province, Institute of Feed Science, Zhejiang University, 310029, Hangzhou, People's Republic of China aut NATIONALLICENCE 700 1- Xie Yonggang National Engineering Laboratory of Biological Feed Safety and Pollution Prevention and Control, The Key Laboratory of Molecular Animal Nutrition of Ministry of Education, The Key Laboratory of Feed and Animal Nutrition of Zhejiang Province, Institute of Feed Science, Zhejiang University, 310029, Hangzhou, People's Republic of China aut NATIONALLICENCE 700 1- Wang Yizhen National Engineering Laboratory of Biological Feed Safety and Pollution Prevention and Control, The Key Laboratory of Molecular Animal Nutrition of Ministry of Education, The Key Laboratory of Feed and Animal Nutrition of Zhejiang Province, Institute of Feed Science, Zhejiang University, 310029, Hangzhou, People's Republic of China aut NATIONALLICENCE 773 0- BioMetals Springer Netherlands 24/6(2011-12-01), 1069-1078 0966-0844 24:6<1069 2011 24 10534 Metadata rights reserved Springer special CC-BY-NC licence nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer