caa a22 4500 445321229 CHVBK 20180317142704.0 cr unu---uuuuu 170323e20111201xx s 000 0 eng 10.1007/s10534-011-9456-z doi (NATIONALLICENCE)springer-10.1007/s10534-011-9456-z Mechanisms underlying the protective effect of zinc and selenium against cadmium-induced oxidative stress in zebrafish Danio rerio [Elektronische Daten] [Mohamed Banni, Lina Chouchene, Khaled Said, Abdelhamid Kerkeni, Imed Messaoudi] The present study was designed to elucidate the protective effect mechanism of Zinc (Zn) and Selenium (Se) on cadmium (Cd)-induced oxidative stress in zebrafish. For this purpose we investigate the response of oxidative stress markers, metallothionein accumulation and gene expression in liver and ovary of female zebrafish exposed to 0,4mg/l Cd in water and supplemented with Zn (5mg kg−1) and/or Se (2mg kg−1) for 21days in their diet. Liver and ovary Cd uptake was evaluated after the exposure period. Cd exposure significantly inhibited the antioxidant enzyme activities termed as catalase (CAT), superoxide dismutase (SOD) and glutathione peroxydase (GPx) and caused a pronounced malondialdehyde (MDA) accumulation in both organs. Co-administration of Zn and Se reversed the Cd-induced toxicity in liver and ovary measured as MDA accumulation. Interestingly, gene expression patterns of Cat, CuZnSod and Gpx were up-regulated when related enzymatic activities were altered. Zebrafish metallothionein transcripts (zMt) significantly decreased in tissues of fish supplemented with Zn and/or Se when compared to Cd-exposed fish. Our data would suggest that Zn and Se protective mechanism against Cd-induced oxidative stress is more depending on the correction of the proteins biological activities rather than on the transcriptional level of related genes. Springer Science+Business Media, LLC., 2011 Oxidative stress nationallicence Gene expression nationallicence Cd nationallicence Zn nationallicence Se nationallicence Zebrafish nationallicence Protective effect nationallicence Banni Mohamed Laboratoire de Biochimie et Toxicologie de l'Environnement (UR04AGR05), ISA, Chott-Mariem, 4042, Sousse, Tunisia aut Chouchene Lina Unité de Recherche: Génétique, Biodiversité et Valorisation des Bioressources, Institut Supérieure de Biotechnologie de Monastir, Monastir, Tunisia aut Said Khaled Unité de Recherche: Génétique, Biodiversité et Valorisation des Bioressources, Institut Supérieure de Biotechnologie de Monastir, Monastir, Tunisia aut Kerkeni Abdelhamid Département de Biophysique, Faculté de Médecine de Monastir, Unité de Recherche: Eléments Traces, Radicaux Libres, Antioxydants, Pathologies Humaines et Environnement, Monastir, Tunisia aut Messaoudi Imed Unité de Recherche: Génétique, Biodiversité et Valorisation des Bioressources, Institut Supérieure de Biotechnologie de Monastir, Monastir, Tunisia aut BioMetals Springer Netherlands 24/6(2011-12-01), 981-992 0966-0844 24:6<981 2011 24 10534 https://doi.org/10.1007/s10534-011-9456-z text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1007/s10534-011-9456-z text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Banni Mohamed Laboratoire de Biochimie et Toxicologie de l'Environnement (UR04AGR05), ISA, Chott-Mariem, 4042, Sousse, Tunisia aut NATIONALLICENCE 700 1- Chouchene Lina Unité de Recherche: Génétique, Biodiversité et Valorisation des Bioressources, Institut Supérieure de Biotechnologie de Monastir, Monastir, Tunisia aut NATIONALLICENCE 700 1- Said Khaled Unité de Recherche: Génétique, Biodiversité et Valorisation des Bioressources, Institut Supérieure de Biotechnologie de Monastir, Monastir, Tunisia aut NATIONALLICENCE 700 1- Kerkeni Abdelhamid Département de Biophysique, Faculté de Médecine de Monastir, Unité de Recherche: Eléments Traces, Radicaux Libres, Antioxydants, Pathologies Humaines et Environnement, Monastir, Tunisia aut NATIONALLICENCE 700 1- Messaoudi Imed Unité de Recherche: Génétique, Biodiversité et Valorisation des Bioressources, Institut Supérieure de Biotechnologie de Monastir, Monastir, Tunisia aut NATIONALLICENCE 773 0- BioMetals Springer Netherlands 24/6(2011-12-01), 981-992 0966-0844 24:6<981 2011 24 10534 Metadata rights reserved Springer special CC-BY-NC licence nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer