caa a22 4500 445321245 CHVBK 20180317142704.0 cr unu---uuuuu 170323e20111201xx s 000 0 eng 10.1007/s10534-011-9464-z doi (NATIONALLICENCE)springer-10.1007/s10534-011-9464-z Pseudomonas siderophores in the sputum of patients with cystic fibrosis [Elektronische Daten] [Lois Martin, David Reid, Katrina Sharples, Iain Lamont] The lungs of patients with cystic fibrosis become chronically infected with the bacterium Pseudomonas aeruginosa, which heralds progressive lung damage and a decline in health. Iron is a crucial micronutrient for bacteria and its acquisition is a key factor in infection. P. aeruginosa can acquire this element by secreting pyoverdine and pyochelin, iron-chelating compounds (siderophores) that scavenge iron and deliver it to the bacteria. Siderophore-mediated iron uptake is generally considered a key factor in the ability of P. aeruginosa to cause infection. We have investigated the amounts of pyoverdine in 148 sputum samples from 36 cystic fibrosis patients (30 infected with P. aeruginosa and 6 as negative controls). Pyoverdine was present in 93 samples in concentrations between 0.30 and 51μM (median 4.6μM) and there was a strong association between the amount of pyoverdine and the number of P. aeruginosa present. However, pyoverdine was not present, or below the limits of detection (~0.3μM), in 21 sputum samples that contained P. aeruginosa. Pyochelin was also absent, or below the limits of detection (~1μM), in samples from P. aeruginosa-infected patients with little or no detectable pyoverdine. Our data show that pyoverdine is an important iron-scavenging molecule for P. aeruginosa in many cystic fibrosis patients, but other P. aeruginosa iron-uptake systems must be active in some patients to satisfy the bacterial need for iron. Springer Science+Business Media, LLC., 2011 Pyoverdine nationallicence Pyochelin nationallicence Iron and infection nationallicence Pseudomonas aeruginosa nationallicence Cystic fibrosis nationallicence CF : Cystic fibrosis nationallicence Martin Lois Department of Biochemistry, University of Otago, P.O. Box 56, Dunedin, New Zealand aut Reid David Menzies Research Institute and University of Tasmania Medical School, 43 Collins Street, 7001, Hobart, TAS, Australia aut Sharples Katrina Department of Preventive and Social Medicine, University of Otago, P.O. Box 56, Dunedin, New Zealand aut Lamont Iain Department of Biochemistry, University of Otago, P.O. Box 56, Dunedin, New Zealand aut BioMetals Springer Netherlands 24/6(2011-12-01), 1059-1067 0966-0844 24:6<1059 2011 24 10534 https://doi.org/10.1007/s10534-011-9464-z text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1007/s10534-011-9464-z text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Martin Lois Department of Biochemistry, University of Otago, P.O. Box 56, Dunedin, New Zealand aut NATIONALLICENCE 700 1- Reid David Menzies Research Institute and University of Tasmania Medical School, 43 Collins Street, 7001, Hobart, TAS, Australia aut NATIONALLICENCE 700 1- Sharples Katrina Department of Preventive and Social Medicine, University of Otago, P.O. Box 56, Dunedin, New Zealand aut NATIONALLICENCE 700 1- Lamont Iain Department of Biochemistry, University of Otago, P.O. Box 56, Dunedin, New Zealand aut NATIONALLICENCE 773 0- BioMetals Springer Netherlands 24/6(2011-12-01), 1059-1067 0966-0844 24:6<1059 2011 24 10534 Metadata rights reserved Springer special CC-BY-NC licence nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer