caa a22 4500 44532127X CHVBK 20180317142704.0 cr unu---uuuuu 170323e20111201xx s 000 0 eng 10.1007/s10534-011-9476-8 doi (NATIONALLICENCE)springer-10.1007/s10534-011-9476-8 Glutathione: a key component of the cytoplasmic labile iron pool [Elektronische Daten] [Robert Hider, Xiao Kong] The cytoplasmic labile iron pool supplies iron to the mitochondrion for heme and iron sulfur cluster synthesis and to many cytoplasmic enzymes, thereby controlling numerous metabolic reactions. Surprisingly the chemical nature of this pool has never been convincingly characterised. Here we provide evidence for iron(II)glutathione being the dominant component of this pool. We report for the first time the affinity constant for the glutathione-iron(II) interaction and use this value to study the cytoplasmic speciation of iron(II). The formation of this complex is a major determinant of the electrode potential of the cytoplasmic ferrous iron pool, a means of selecting between iron(II) and manganese(II) and it provides a substrate for glutaredoxin/iron clusters at the dimer interface of glutaredoxins involved in the synthesis of Fe-S cluster proteins. Springer Science+Business Media, LLC., 2011 Iron nationallicence Glutathione nationallicence Cytoplasmic labile iron pool nationallicence Hider Robert Institute of Pharmaceutical Science, King's College London, Franklin-Wilkins Building, 150 Stamford Street, SE1 9NH, London, UK aut Kong Xiao Institute of Pharmaceutical Science, King's College London, Franklin-Wilkins Building, 150 Stamford Street, SE1 9NH, London, UK aut BioMetals Springer Netherlands 24/6(2011-12-01), 1179-1187 0966-0844 24:6<1179 2011 24 10534 https://doi.org/10.1007/s10534-011-9476-8 text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1007/s10534-011-9476-8 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Hider Robert Institute of Pharmaceutical Science, King's College London, Franklin-Wilkins Building, 150 Stamford Street, SE1 9NH, London, UK aut NATIONALLICENCE 700 1- Kong Xiao Institute of Pharmaceutical Science, King's College London, Franklin-Wilkins Building, 150 Stamford Street, SE1 9NH, London, UK aut NATIONALLICENCE 773 0- BioMetals Springer Netherlands 24/6(2011-12-01), 1179-1187 0966-0844 24:6<1179 2011 24 10534 Metadata rights reserved Springer special CC-BY-NC licence nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer