caa a22 4500 445321288 CHVBK 20180317142704.0 cr unu---uuuuu 170323e20111201xx s 000 0 eng 10.1007/s10534-011-9477-7 doi (NATIONALLICENCE)springer-10.1007/s10534-011-9477-7 The metal-coordinated Casiopeína IIIEa induces the petite-like phenotype in Saccharomyces cerevisiae [Elektronische Daten] [Angélica López-Rodríguez, Alfonso Cárabez-Trejo, Fernando Rosas-Sánchez, Carmen Mejía, Lena Ruiz-Azuara, Ricardo Miledi, Ataúlfo Martínez-Torres] The Casiopeínas® are mixed chelate copper (II) complexes and promising antineoplastics agents against cancer cells and tumors in vitro and in vivo. However, the action mode of these compounds is poorly characterized. In this work the effect of the antineoplastic Casiopeína IIIEa on the metabolism and ultrastructure of the yeast Saccharomyces cerevisiae was investigated. Exposure of cells growing in rich or in low-iron medium to 5μM of the compound decreased duplication time and reduced oxygen consumption. Those cells formed smaller colonies when growing in a non-fermentable carbon source and low-iron medium, and under the light microscope, multiple folds were observed along the plasma membrane accompanied with a reduction in the diameter of the yeast. These observations were confirmed under the electron microscope, which also revealed a slight reduction of the mitochondrial size. A correlation was found with smaller colonies exhibiting lower rates of oxygen consumption, and yeast labelled with fluorescent MitoTrackerTM consistently exhibited reduced mitochondrial activity. It appears that Casiopeína IIIEa gives rise to smaller yeast and petite-like colonies by reducing the mitochondrial respiratory activity without significantly affecting the mitochondrial structure. Springer Science+Business Media, LLC., 2011 Yeast nationallicence Petite colonies nationallicence Mitochondria nationallicence Respiratory activity nationallicence Casiopeínas nationallicence Copper compounds nationallicence López-Rodríguez Angélica Departamento de Neurobiología Celular y Molecular, Instituto de Neurobiología, UNAM-Campus Juriquilla, Boulevard Juriquilla 3001, C.P. 76230, Juriquilla, Querétaro, México aut Cárabez-Trejo Alfonso Departamento de Neurobiología Celular y Molecular, Instituto de Neurobiología, UNAM-Campus Juriquilla, Boulevard Juriquilla 3001, C.P. 76230, Juriquilla, Querétaro, México aut Rosas-Sánchez Fernando Departamento de Neurobiología Celular y Molecular, Instituto de Neurobiología, UNAM-Campus Juriquilla, Boulevard Juriquilla 3001, C.P. 76230, Juriquilla, Querétaro, México aut Mejía Carmen Instituto de Investigaciones Biomédicas, UNAM y, México D.F., México aut Ruiz-Azuara Lena Departamento de Química Inorgánica y Nuclear, Facultad de Química, UNAM, México D.F., México aut Miledi Ricardo Departamento de Neurobiología Celular y Molecular, Instituto de Neurobiología, UNAM-Campus Juriquilla, Boulevard Juriquilla 3001, C.P. 76230, Juriquilla, Querétaro, México aut Martínez-Torres Ataúlfo Departamento de Neurobiología Celular y Molecular, Instituto de Neurobiología, UNAM-Campus Juriquilla, Boulevard Juriquilla 3001, C.P. 76230, Juriquilla, Querétaro, México aut BioMetals Springer Netherlands 24/6(2011-12-01), 1189-1196 0966-0844 24:6<1189 2011 24 10534 https://doi.org/10.1007/s10534-011-9477-7 text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1007/s10534-011-9477-7 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- López-Rodríguez Angélica Departamento de Neurobiología Celular y Molecular, Instituto de Neurobiología, UNAM-Campus Juriquilla, Boulevard Juriquilla 3001, C.P. 76230, Juriquilla, Querétaro, México aut NATIONALLICENCE 700 1- Cárabez-Trejo Alfonso Departamento de Neurobiología Celular y Molecular, Instituto de Neurobiología, UNAM-Campus Juriquilla, Boulevard Juriquilla 3001, C.P. 76230, Juriquilla, Querétaro, México aut NATIONALLICENCE 700 1- Rosas-Sánchez Fernando Departamento de Neurobiología Celular y Molecular, Instituto de Neurobiología, UNAM-Campus Juriquilla, Boulevard Juriquilla 3001, C.P. 76230, Juriquilla, Querétaro, México aut NATIONALLICENCE 700 1- Mejía Carmen Instituto de Investigaciones Biomédicas, UNAM y, México D.F., México aut NATIONALLICENCE 700 1- Ruiz-Azuara Lena Departamento de Química Inorgánica y Nuclear, Facultad de Química, UNAM, México D.F., México aut NATIONALLICENCE 700 1- Miledi Ricardo Departamento de Neurobiología Celular y Molecular, Instituto de Neurobiología, UNAM-Campus Juriquilla, Boulevard Juriquilla 3001, C.P. 76230, Juriquilla, Querétaro, México aut NATIONALLICENCE 700 1- Martínez-Torres Ataúlfo Departamento de Neurobiología Celular y Molecular, Instituto de Neurobiología, UNAM-Campus Juriquilla, Boulevard Juriquilla 3001, C.P. 76230, Juriquilla, Querétaro, México aut NATIONALLICENCE 773 0- BioMetals Springer Netherlands 24/6(2011-12-01), 1189-1196 0966-0844 24:6<1189 2011 24 10534 Metadata rights reserved Springer special CC-BY-NC licence nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer