caa a22 4500 445321296 CHVBK 20180317142704.0 cr unu---uuuuu 170323e20111201xx s 000 0 eng 10.1007/s10534-011-9461-2 doi (NATIONALLICENCE)springer-10.1007/s10534-011-9461-2 Supplementation of zinc mitigates the altered uptake and turnover of 65Zn in liver and whole body of diabetic rats [Elektronische Daten] [Ashima Pathak, Vishawjyoti Sharma, Sanjeev Kumar, D. Dhawan] Diabetes is a life threatening disease and its onset is linked with both environmental and genetic factors. Zinc metabolism gets altered during diabetes and results in many complications. The present study was designed to elucidate the effects of zinc supplementation on the biokinetics of 65Zn in whole body, liver and its biodistribution in diabetic rats. The animals were divided into four groups viz; normal control; diabetic (single intraperitoneal injection of alloxan 150mg/kg body weight); zinc treated (227mg/l in drinking water); and diabetic+zinc treated. To carry out biokinetics study, each rat was injected intraperitoneally with 0.74MBq radioactivity of 65Zn following 4weeks of different treatments and the radioactivity was determined by using a suitably shielded scintillation counter. Alloxan induced diabetic rats showed a significant decrease in both the fast (Tb1) and slow (Tb2) components of biological half-life of 65Zn which, however, were normalized in whole body (P>0.05) following zinc supplementation. In case of liver, Tb2 component was brought back to the normal but Tb1 component was not increased significantly. The present study indicates that the paucity of zinc in the tissues of the diabetic animals was due to decreased retention of tissue zinc as evidenced by increased serum Zn, hyperzincuria and increased rate of uptake of 65Zn by the liver. Zinc supplementation caused a significant improvement in the retention of zinc in the tissues and is therefore likely to be of benefit in the treatment of diabetes. Springer Science+Business Media, LLC., 2011 Diabetes nationallicence Zinc supplementation nationallicence Biokinetics of 65Zn nationallicence 65Zn uptake nationallicence Hyperzincuria nationallicence Pathak Ashima Department of Biotechnology, GGDSD College, Sector-32, Chandigarh, India aut Sharma Vishawjyoti Department of Biophysics, Panjab University, 160014, Chandigarh, India aut Kumar Sanjeev Department of Biophysics, Panjab University, 160014, Chandigarh, India aut Dhawan D. Department of Biophysics, Panjab University, 160014, Chandigarh, India aut BioMetals Springer Netherlands 24/6(2011-12-01), 1027-1034 0966-0844 24:6<1027 2011 24 10534 https://doi.org/10.1007/s10534-011-9461-2 text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1007/s10534-011-9461-2 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Pathak Ashima Department of Biotechnology, GGDSD College, Sector-32, Chandigarh, India aut NATIONALLICENCE 700 1- Sharma Vishawjyoti Department of Biophysics, Panjab University, 160014, Chandigarh, India aut NATIONALLICENCE 700 1- Kumar Sanjeev Department of Biophysics, Panjab University, 160014, Chandigarh, India aut NATIONALLICENCE 700 1- Dhawan D. Department of Biophysics, Panjab University, 160014, Chandigarh, India aut NATIONALLICENCE 773 0- BioMetals Springer Netherlands 24/6(2011-12-01), 1027-1034 0966-0844 24:6<1027 2011 24 10534 Metadata rights reserved Springer special CC-BY-NC licence nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer