caa a22 4500 44532130X CHVBK 20180317142704.0 cr unu---uuuuu 170323e20111201xx s 000 0 eng 10.1007/s10534-011-9463-0 doi (NATIONALLICENCE)springer-10.1007/s10534-011-9463-0 Acute and chronic metal exposure impairs locomotion activity in Drosophila melanogaster [Elektronische Daten] a model to study Parkinsonism [Leonardo Bonilla-Ramirez, Marlene Jimenez-Del-Rio, Carlos Velez-Pardo] The biometals iron (Fe), manganese (Mn) and copper (Cu) have been associated to Parkinson's disease (PD) and Parkinsonism. In this work, we report for the first time that acute (15mM for up to 5days) or chronic (0.5mM for up to 15days) Fe, Mn and Cu exposure significantly reduced life span and locomotor activity (i.e. climbing capabilities) in Drosophila melanogaster. It is shown that the concentration of those biometals dramatically increase in Drosophila's brain acutely or chronically fed with metal. We demonstrate that the metal accumulation in the fly's head is associated with the neurodegeneration of several dopaminergic neuronal clusters. Interestingly, it is found that the PPL2ab DAergic neuronal cluster was erode by the three metals in acute and chronic metal exposure and the PPL3 DAergic cluster was also erode by the three metals but in acute metal exposure only. Furthermore, we found that the chelator desferoxamine, ethylenediaminetetraacetic acid, and d-penicillamine were able to protect but not rescue D. melanogaster against metal intoxication. Taken together these data suggest that iron, manganese and copper are capable to destroy DAergic neurons in the fly's brain, thereby impairing their movement capabilities. This work provides for the first time metal-induced Parkinson-like symptoms in D. melanogaster. Understanding therefore the effects of biometals in the Drosophila model may provide insights into the toxic effect of metal ions and more effective therapeutic approaches to Parkinsonism. Springer Science+Business Media, LLC., 2011 Copper nationallicence Drosophila melanogaster nationallicence Manganese nationallicence Parkinson nationallicence Parkinsonism nationallicence Iron nationallicence Bonilla-Ramirez Leonardo School of Medicine, Medical Research Institute, Neuroscience Research Group, University of Antioquia (UdeA), Calle 62 # 52-59, Building 1, Laboratory 412, SIU, Medellin, Colombia aut Jimenez-Del-Rio Marlene School of Medicine, Medical Research Institute, Neuroscience Research Group, University of Antioquia (UdeA), Calle 62 # 52-59, Building 1, Laboratory 412, SIU, Medellin, Colombia aut Velez-Pardo Carlos School of Medicine, Medical Research Institute, Neuroscience Research Group, University of Antioquia (UdeA), Calle 62 # 52-59, Building 1, Laboratory 412, SIU, Medellin, Colombia aut BioMetals Springer Netherlands 24/6(2011-12-01), 1045-1057 0966-0844 24:6<1045 2011 24 10534 https://doi.org/10.1007/s10534-011-9463-0 text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1007/s10534-011-9463-0 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Bonilla-Ramirez Leonardo School of Medicine, Medical Research Institute, Neuroscience Research Group, University of Antioquia (UdeA), Calle 62 # 52-59, Building 1, Laboratory 412, SIU, Medellin, Colombia aut NATIONALLICENCE 700 1- Jimenez-Del-Rio Marlene School of Medicine, Medical Research Institute, Neuroscience Research Group, University of Antioquia (UdeA), Calle 62 # 52-59, Building 1, Laboratory 412, SIU, Medellin, Colombia aut NATIONALLICENCE 700 1- Velez-Pardo Carlos School of Medicine, Medical Research Institute, Neuroscience Research Group, University of Antioquia (UdeA), Calle 62 # 52-59, Building 1, Laboratory 412, SIU, Medellin, Colombia aut NATIONALLICENCE 773 0- BioMetals Springer Netherlands 24/6(2011-12-01), 1045-1057 0966-0844 24:6<1045 2011 24 10534 Metadata rights reserved Springer special CC-BY-NC licence nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer