caa a22 4500 445321350 CHVBK 20180317142705.0 cr unu---uuuuu 170323e20111201xx s 000 0 eng 10.1007/s10534-011-9473-y doi (NATIONALLICENCE)springer-10.1007/s10534-011-9473-y Heavy metal resistance in Cupriavidus metallidurans CH34 is governed by an intricate transcriptional network [Elektronische Daten] [Pieter Monsieurs, Hugo Moors, Rob Van Houdt, Paul Janssen, Ann Janssen, Ilse Coninx, Max Mergeay, Natalie Leys] The soil bacterium Cupriavidus metallidurans CH34 contains a high number of heavy metal resistance genes making it an interesting model organism to study microbial responses to heavy metals. In this study the transcriptional response of strain CH34 was measured when challenged to sub-lethal concentrations of various essential or toxic metals. Based on the global transcriptional responses for each challenge and the overlap in upregulated genes between different metal responses, the sixteen metals were clustered in three groups. In addition, the transcriptional response of already known metal resistance genes was assessed, and new metal response gene clusters were identified. The majority of the studied metal response loci showed similar expression profiles when cells were exposed to different metals, suggesting complex interplay at transcriptional level between the different metal responses. The pronounced redundancy of these metal resistant regions—as illustrated by the large number of paralogous genes—combined with the phylogenetic distribution of these metal response regions within either evolutionary related or other metal resistant bacteria, provides important insights on the recent evolutionary forces shaping this naturally soil-dwelling bacterium into a highly metal-resistant strain well adapted to harsh and anthropogenic environments. Springer Science+Business Media, LLC., 2011 Transcriptomics nationallicence Cupriavidus metallidurans CH34 nationallicence Transcriptional regulation nationallicence Metal resistance nationallicence Regulatory networks nationallicence Monsieurs Pieter Molecular and Cellular Biology, Belgian Nuclear Research Centre (SCK·CEN), 2400 Mol, Boeretang 200, Belgium aut Moors Hugo Molecular and Cellular Biology, Belgian Nuclear Research Centre (SCK·CEN), 2400 Mol, Boeretang 200, Belgium aut Van Houdt Rob Molecular and Cellular Biology, Belgian Nuclear Research Centre (SCK·CEN), 2400 Mol, Boeretang 200, Belgium aut Janssen Paul Molecular and Cellular Biology, Belgian Nuclear Research Centre (SCK·CEN), 2400 Mol, Boeretang 200, Belgium aut Janssen Ann Molecular and Cellular Biology, Belgian Nuclear Research Centre (SCK·CEN), 2400 Mol, Boeretang 200, Belgium aut Coninx Ilse Molecular and Cellular Biology, Belgian Nuclear Research Centre (SCK·CEN), 2400 Mol, Boeretang 200, Belgium aut Mergeay Max Molecular and Cellular Biology, Belgian Nuclear Research Centre (SCK·CEN), 2400 Mol, Boeretang 200, Belgium aut Leys Natalie Molecular and Cellular Biology, Belgian Nuclear Research Centre (SCK·CEN), 2400 Mol, Boeretang 200, Belgium aut BioMetals Springer Netherlands 24/6(2011-12-01), 1133-1151 0966-0844 24:6<1133 2011 24 10534 https://doi.org/10.1007/s10534-011-9473-y text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1007/s10534-011-9473-y text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Monsieurs Pieter Molecular and Cellular Biology, Belgian Nuclear Research Centre (SCK·CEN), 2400 Mol, Boeretang 200, Belgium aut NATIONALLICENCE 700 1- Moors Hugo Molecular and Cellular Biology, Belgian Nuclear Research Centre (SCK·CEN), 2400 Mol, Boeretang 200, Belgium aut NATIONALLICENCE 700 1- Van Houdt Rob Molecular and Cellular Biology, Belgian Nuclear Research Centre (SCK·CEN), 2400 Mol, Boeretang 200, Belgium aut NATIONALLICENCE 700 1- Janssen Paul Molecular and Cellular Biology, Belgian Nuclear Research Centre (SCK·CEN), 2400 Mol, Boeretang 200, Belgium aut NATIONALLICENCE 700 1- Janssen Ann Molecular and Cellular Biology, Belgian Nuclear Research Centre (SCK·CEN), 2400 Mol, Boeretang 200, Belgium aut NATIONALLICENCE 700 1- Coninx Ilse Molecular and Cellular Biology, Belgian Nuclear Research Centre (SCK·CEN), 2400 Mol, Boeretang 200, Belgium aut NATIONALLICENCE 700 1- Mergeay Max Molecular and Cellular Biology, Belgian Nuclear Research Centre (SCK·CEN), 2400 Mol, Boeretang 200, Belgium aut NATIONALLICENCE 700 1- Leys Natalie Molecular and Cellular Biology, Belgian Nuclear Research Centre (SCK·CEN), 2400 Mol, Boeretang 200, Belgium aut NATIONALLICENCE 773 0- BioMetals Springer Netherlands 24/6(2011-12-01), 1133-1151 0966-0844 24:6<1133 2011 24 10534 Metadata rights reserved Springer special CC-BY-NC licence nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer