caa a22 4500 445321369 CHVBK 20180317142705.0 cr unu---uuuuu 170323e20111201xx s 000 0 eng 10.1007/s10534-011-9474-x doi (NATIONALLICENCE)springer-10.1007/s10534-011-9474-x Cyto- and genotoxicity of a vanadyl(IV) complex with oxodiacetate in human colon adenocarcinoma (Caco-2) cells: potential use in cancer therapy [Elektronische Daten] [Ana Di Virgilio, Josefina Rivadeneira, Cecilia Muglia, Miguel Reigosa, Nataliya Butenko, Isabel Cavaco, Susana Etcheverry] The complex of vanadyl(IV) cation with oxodiacetate, VO(oda) caused an inhibitory effect on the proliferation of the human colon adenocarcinoma cell line Caco-2 in the range of 25-100μM (P<0.001). This inhibition was partially reversed by scavengers of free radicals. The difference in cell proliferation in the presence and the absence of scavengers was statistically significant in the range of 50-100μM (P<0.05). VO(oda) altered lysosomal and mitochondria metabolisms (neutral red and MTT bioassays) in a dose-response manner from 10μM (P<0.001). Morphological studies showed important transformations that correlated with the disassembly of actin filaments and a decrease in the number of cells in a dose response manner. Moreover, VO(oda) caused statistically significant genotoxic effects on Caco-2 cells in the low range of concentration (5-25μM) (Comet assay). Increment in the oxidative stress and a decrease in the GSH level are the main cytotoxic mechanisms of VO(oda). These effects were partially reversed by scavengers of free radicals in the range of 50-100μM (P<0.05). Besides, VO(oda) interacted with plasmidic DNA causing single and double strand cleavage, probably through the action of free radical species. Altogether, these results suggest that VO(oda) is a good candidate to be evaluated for alternative therapeutics in cancer treatment. Springer Science+Business Media, LLC., 2011 Vanadyl(IV) cation nationallicence Multidentate ligands nationallicence Cytotoxicity nationallicence Genotoxicity nationallicence Caco-2 tumoral cells nationallicence Di Virgilio Ana Cátedra de Bioquímica Patológica, Facultad de Ciencias Exactas, UNLP, 47 y 115 (1900), La Plata, Argentina aut Rivadeneira Josefina Cátedra de Bioquímica Patológica, Facultad de Ciencias Exactas, UNLP, 47 y 115 (1900), La Plata, Argentina aut Muglia Cecilia Cátedra de Bioquímica Patológica, Facultad de Ciencias Exactas, UNLP, 47 y 115 (1900), La Plata, Argentina aut Reigosa Miguel Instituto Multidisciplinario de Biología Celular (IMBICE), 526 y Cno. Gral. Belgrano, La Plata, Argentina aut Butenko Nataliya Departamento de Química, Bioquímica e Farmácia, Universidade do Algarve, Campus de Gambelas, 8005-139, Faro, Portugal aut Cavaco Isabel Departamento de Química, Bioquímica e Farmácia, Universidade do Algarve, Campus de Gambelas, 8005-139, Faro, Portugal aut Etcheverry Susana Cátedra de Bioquímica Patológica, Facultad de Ciencias Exactas, UNLP, 47 y 115 (1900), La Plata, Argentina aut BioMetals Springer Netherlands 24/6(2011-12-01), 1153-1168 0966-0844 24:6<1153 2011 24 10534 https://doi.org/10.1007/s10534-011-9474-x text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1007/s10534-011-9474-x text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Di Virgilio Ana Cátedra de Bioquímica Patológica, Facultad de Ciencias Exactas, UNLP, 47 y 115 (1900), La Plata, Argentina aut NATIONALLICENCE 700 1- Rivadeneira Josefina Cátedra de Bioquímica Patológica, Facultad de Ciencias Exactas, UNLP, 47 y 115 (1900), La Plata, Argentina aut NATIONALLICENCE 700 1- Muglia Cecilia Cátedra de Bioquímica Patológica, Facultad de Ciencias Exactas, UNLP, 47 y 115 (1900), La Plata, Argentina aut NATIONALLICENCE 700 1- Reigosa Miguel Instituto Multidisciplinario de Biología Celular (IMBICE), 526 y Cno. Gral. Belgrano, La Plata, Argentina aut NATIONALLICENCE 700 1- Butenko Nataliya Departamento de Química, Bioquímica e Farmácia, Universidade do Algarve, Campus de Gambelas, 8005-139, Faro, Portugal aut NATIONALLICENCE 700 1- Cavaco Isabel Departamento de Química, Bioquímica e Farmácia, Universidade do Algarve, Campus de Gambelas, 8005-139, Faro, Portugal aut NATIONALLICENCE 700 1- Etcheverry Susana Cátedra de Bioquímica Patológica, Facultad de Ciencias Exactas, UNLP, 47 y 115 (1900), La Plata, Argentina aut NATIONALLICENCE 773 0- BioMetals Springer Netherlands 24/6(2011-12-01), 1153-1168 0966-0844 24:6<1153 2011 24 10534 Metadata rights reserved Springer special CC-BY-NC licence nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer