caa a22 4500 445321385 CHVBK 20180317142705.0 cr unu---uuuuu 170323e20111201xx s 000 0 eng 10.1007/s10534-011-9462-1 doi (NATIONALLICENCE)springer-10.1007/s10534-011-9462-1 Shell-bound iron dependant nitric oxide synthesis in encysted Artemia parthenogenetica embryos during hydrogen peroxide exposure [Elektronische Daten] [Sivaram Veeramani, Vaseeharan Baskaralingam] Artemia is a tiny marine crustacean, serves as an excellent tool in both basic and applied aspects of stress biology research. In the current manuscript, we report that Artemia parthenogenetica embryos (cysts), in diapause stage, undergo iron transition changes when exposed to chemical diapause deactivation stimulus (hydrogen peroxide). X-ray surface analysis of A. parthenogenetica embryos exposed to H2O2 showed significant transitional changes in iron, as seen in cyst cross-sections. Electron paramagnetic resonance study revealed that upon H2O2 exposure, increased nitric oxide (NO) production was observed in non-decapsulated cysts (ND), but not in decapsulated cysts (DC) (shell-removed cysts). Spin trapping studies also showed an increase in hydroxyl radical formation in NDs exposed to H2O2 through Fenton-like reaction. On the contrary, exposure of DCs to H2O2 did not induce hydroxyl radical formation. Taken together, results from the present study indicate a key role of cyst shell-bound iron and reactive oxygen species on successful diapause termination in eukaryotic extremophile animal model, such as Artemia. Springer Science+Business Media, LLC., 2011 Artemia parthenogenetica nationallicence Diapause nationallicence Hydrogen peroxide nationallicence Nitric oxide nationallicence Shell-bound Iron nationallicence Veeramani Sivaram Department of Animal Health Management, Alagappa University, 630 003, Karaikudi, Tamil Nadu, India aut Baskaralingam Vaseeharan Department of Animal Health Management, Alagappa University, 630 003, Karaikudi, Tamil Nadu, India aut BioMetals Springer Netherlands 24/6(2011-12-01), 1035-1044 0966-0844 24:6<1035 2011 24 10534 https://doi.org/10.1007/s10534-011-9462-1 text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1007/s10534-011-9462-1 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Veeramani Sivaram Department of Animal Health Management, Alagappa University, 630 003, Karaikudi, Tamil Nadu, India aut NATIONALLICENCE 700 1- Baskaralingam Vaseeharan Department of Animal Health Management, Alagappa University, 630 003, Karaikudi, Tamil Nadu, India aut NATIONALLICENCE 773 0- BioMetals Springer Netherlands 24/6(2011-12-01), 1035-1044 0966-0844 24:6<1035 2011 24 10534 Metadata rights reserved Springer special CC-BY-NC licence nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer