caa a22 4500 445321393 CHVBK 20180317142705.0 cr unu---uuuuu 170323e20111201xx s 000 0 eng 10.1007/s10534-011-9466-x doi (NATIONALLICENCE)springer-10.1007/s10534-011-9466-x Physiological relevance and contribution to metal balance of specific and non-specific Metallothionein isoforms in the garden snail, Cantareus aspersus [Elektronische Daten] [Martina Höckner, Karin Stefanon, Annette de Vaufleury, Freddy Monteiro, Sílvia Pérez-Rafael, Òscar Palacios, Mercè Capdevila, Sílvia Atrian, Reinhard Dallinger] Variable environmental availability of metal ions represents a constant challenge for most organisms, so that during evolution, they have optimised physiological and molecular mechanisms to cope with this particular requirement. Metallothioneins (MTs) are proteins that play a major role in metal homeostasis and as a reservoir. The MT gene/protein systems of terrestrial helicid snails are an invaluable model for the study of metal-binding features and MT isoform-specific functionality of these proteins. In the present study, we characterised three paralogous MT isogenes and their expressed products in the escargot (Cantareus aspersus). The metal-dependent transcriptional activation of the three isogenes was assessed using quantitative Real Time PCR. The metal-binding capacities of the three isoforms were studied by characterising the purified native complexes. All the data were analysed in relation to the trace element status of the animals after metal feeding. Two of the three C. aspersus MT (CaMT) isoforms appeared to be metal-specific, (CaCdMT and CaCuMT, for cadmium and copper respectively). A third isoform (CaCd/CuMT) was non-specific, since it was natively recovered as a mixed Cd/Cu complex. A specific role in Cd detoxification for CaCdMT was revealed, with a 80-90% contribution to the Cd balance in snails exposed to this metal. Conclusive data were also obtained for the CaCuMT isoform, which is involved in Cu homeostasis, sharing about 30-50% of the Cu balance of C. aspersus. No apparent metal-related physiological function was found for the third isoform (CaCd/CuMT), so its contribution to the metal balance of the escargot may be, if at all, of only marginal significance, but may enclose a major interest in evolutionary studies. Springer Science+Business Media, LLC., 2011 Cadmium nationallicence Copper nationallicence Zinc nationallicence Metallothionein nationallicence Mollusca nationallicence Pulmonata nationallicence AAS : Atomic absorption spectrophotometry nationallicence CaMT : C. aspersus MT (global denomination) nationallicence CaCdMT : Cd-specific C. aspersus MT isoform nationallicence CaCuMT : Cu-specific C. aspersus MT isoform nationallicence CaCd/CuMT : Mixed metal C. aspersus MT isoform nationallicence ESI-MS : Electrospray ionization mass spectrometry nationallicence ESI-TOF MS : Time-of-flight electrospray ionization mass spectrometry nationallicence Höckner Martina Institute of Zoology, University of Innsbruck, 6020, Innsbruck, Austria aut Stefanon Karin Institute of Zoology, University of Innsbruck, 6020, Innsbruck, Austria aut de Vaufleury Annette Department of Chrono-Environment, University of Franche-Comté, 25030, Besançon Cedex, France aut Monteiro Freddy Departament de Genètica, Facultat de Biologia, Universitat de Barcelona, 08028, Barcelona, Spain aut Pérez-Rafael Sílvia Departament de Química, Facultat de Ciències, Universitat Autònoma de Barcelona, 08193, Barcelona, Bellaterra, Spain aut Palacios Òscar Departament de Química, Facultat de Ciències, Universitat Autònoma de Barcelona, 08193, Barcelona, Bellaterra, Spain aut Capdevila Mercè Departament de Química, Facultat de Ciències, Universitat Autònoma de Barcelona, 08193, Barcelona, Bellaterra, Spain aut Atrian Sílvia Departament de Genètica, Facultat de Biologia, Universitat de Barcelona, 08028, Barcelona, Spain aut Dallinger Reinhard Institute of Zoology, University of Innsbruck, 6020, Innsbruck, Austria aut BioMetals Springer Netherlands 24/6(2011-12-01), 1079-1092 0966-0844 24:6<1079 2011 24 10534 https://doi.org/10.1007/s10534-011-9466-x text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1007/s10534-011-9466-x text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Höckner Martina Institute of Zoology, University of Innsbruck, 6020, Innsbruck, Austria aut NATIONALLICENCE 700 1- Stefanon Karin Institute of Zoology, University of Innsbruck, 6020, Innsbruck, Austria aut NATIONALLICENCE 700 1- de Vaufleury Annette Department of Chrono-Environment, University of Franche-Comté, 25030, Besançon Cedex, France aut NATIONALLICENCE 700 1- Monteiro Freddy Departament de Genètica, Facultat de Biologia, Universitat de Barcelona, 08028, Barcelona, Spain aut NATIONALLICENCE 700 1- Pérez-Rafael Sílvia Departament de Química, Facultat de Ciències, Universitat Autònoma de Barcelona, 08193, Barcelona, Bellaterra, Spain aut NATIONALLICENCE 700 1- Palacios Òscar Departament de Química, Facultat de Ciències, Universitat Autònoma de Barcelona, 08193, Barcelona, Bellaterra, Spain aut NATIONALLICENCE 700 1- Capdevila Mercè Departament de Química, Facultat de Ciències, Universitat Autònoma de Barcelona, 08193, Barcelona, Bellaterra, Spain aut NATIONALLICENCE 700 1- Atrian Sílvia Departament de Genètica, Facultat de Biologia, Universitat de Barcelona, 08028, Barcelona, Spain aut NATIONALLICENCE 700 1- Dallinger Reinhard Institute of Zoology, University of Innsbruck, 6020, Innsbruck, Austria aut NATIONALLICENCE 773 0- BioMetals Springer Netherlands 24/6(2011-12-01), 1079-1092 0966-0844 24:6<1079 2011 24 10534 Metadata rights reserved Springer special CC-BY-NC licence nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer