caa a22 4500 445321407 CHVBK 20180317142705.0 cr unu---uuuuu 170323e20111201xx s 000 0 eng 10.1007/s10534-011-9459-9 doi (NATIONALLICENCE)springer-10.1007/s10534-011-9459-9 Compartmentalization and ultrastructural alterations induced by chromium in aquatic macrophytes [Elektronische Daten] [Pedro Mangabeira, Aluane Ferreira, Alex-Alan de Almeida, Valéria Fernandes, Emerson Lucena, Vânia Souza, Alberto dos Santos Júnior, Arno Oliveira, Marie Grenier-Loustalot, Fréderique Barbier, Delmira Silva] The aim of the present study was to identify the sites of accumulation of Cr in the species of macrophytes that are abundant in the Cachoeira river, namely, Alternanthera philoxeroides, Borreria scabiosoides, Polygonum ferrugineum and Eichhornia crassipes. Plants were grown in nutritive solution supplemented with 0.25 and 50mgl−1 of CrCl3·6H2O. Samples of plant tissues were digested with HNO3/HCl in a closed-vessel microwave system and the concentrations of Cr determined using inductively-coupled plasma mass spectrometry (ICP-MS). The ultrastructure of root, stem and leaf tissue was examined using transmission electron microscopy (TEM) and secondary ion mass spectrometry (SIMS) in order to determine the sites of accumulation of Cr and to detect possible alterations in cell organelles induced by the presence of the metal. Chromium accumulated principally in the roots of the four macrophytes (8.6-30mgkg−1 dw), with much lower concentrations present in the stems and leaves (3.8-8.6 and 0.01-9.0mgkg−1 dw, respectively). Within root tissue, Cr was present mainly in the vacuoles of parenchyma cells and cell walls of xylem and parenchyma. Alterations in the shape of the chloroplasts and nuclei were detected in A. philoxeroides and B. scabiosoides, suggesting a possible application of these aquatic plants as biomarkers from Cr contamination. Springer Science+Business Media, LLC., 2011 Aquatic plants nationallicence Heavy metals nationallicence Chromium nationallicence Cell ultrastructure nationallicence Transmission electron microscopy nationallicence Secondary ion mass spectrometry nationallicence Mangabeira Pedro Departamento de Ciências Biológicas, Centro de Microscopia Eletrônica, Universidade Estadual de Santa Cruz, Rodovia Ilhéus-Itabuna km 16, 45662-900, Ilhéus, Bahia, Brazil aut Ferreira Aluane Departamento de Ciências Biológicas, Centro de Microscopia Eletrônica, Universidade Estadual de Santa Cruz, Rodovia Ilhéus-Itabuna km 16, 45662-900, Ilhéus, Bahia, Brazil aut de Almeida Alex-Alan Departamento de Ciências Biológicas, Centro de Microscopia Eletrônica, Universidade Estadual de Santa Cruz, Rodovia Ilhéus-Itabuna km 16, 45662-900, Ilhéus, Bahia, Brazil aut Fernandes Valéria Departamento de Ciências Biológicas, Centro de Microscopia Eletrônica, Universidade Estadual de Santa Cruz, Rodovia Ilhéus-Itabuna km 16, 45662-900, Ilhéus, Bahia, Brazil aut Lucena Emerson Departamento de Ciências Biológicas, Centro de Microscopia Eletrônica, Universidade Estadual de Santa Cruz, Rodovia Ilhéus-Itabuna km 16, 45662-900, Ilhéus, Bahia, Brazil aut Souza Vânia Departamento de Ciências Biológicas, Centro de Microscopia Eletrônica, Universidade Estadual de Santa Cruz, Rodovia Ilhéus-Itabuna km 16, 45662-900, Ilhéus, Bahia, Brazil aut dos Santos Júnior Alberto Departamento de Ciências Biológicas, Centro de Microscopia Eletrônica, Universidade Estadual de Santa Cruz, Rodovia Ilhéus-Itabuna km 16, 45662-900, Ilhéus, Bahia, Brazil aut Oliveira Arno Departamento de Ciências Biológicas, Centro de Microscopia Eletrônica, Universidade Estadual de Santa Cruz, Rodovia Ilhéus-Itabuna km 16, 45662-900, Ilhéus, Bahia, Brazil aut Grenier-Loustalot Marie Service Central d'Analyse, CNRS, Echangeur de Solaize, Chemin du Canal, 69360, Solaize, France aut Barbier Fréderique Service Central d'Analyse, CNRS, Echangeur de Solaize, Chemin du Canal, 69360, Solaize, France aut Silva Delmira Departamento de Ciências Biológicas, Centro de Microscopia Eletrônica, Universidade Estadual de Santa Cruz, Rodovia Ilhéus-Itabuna km 16, 45662-900, Ilhéus, Bahia, Brazil aut BioMetals Springer Netherlands 24/6(2011-12-01), 1017-1026 0966-0844 24:6<1017 2011 24 10534 https://doi.org/10.1007/s10534-011-9459-9 text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1007/s10534-011-9459-9 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Mangabeira Pedro Departamento de Ciências Biológicas, Centro de Microscopia Eletrônica, Universidade Estadual de Santa Cruz, Rodovia Ilhéus-Itabuna km 16, 45662-900, Ilhéus, Bahia, Brazil aut NATIONALLICENCE 700 1- Ferreira Aluane Departamento de Ciências Biológicas, Centro de Microscopia Eletrônica, Universidade Estadual de Santa Cruz, Rodovia Ilhéus-Itabuna km 16, 45662-900, Ilhéus, Bahia, Brazil aut NATIONALLICENCE 700 1- de Almeida Alex-Alan Departamento de Ciências Biológicas, Centro de Microscopia Eletrônica, Universidade Estadual de Santa Cruz, Rodovia Ilhéus-Itabuna km 16, 45662-900, Ilhéus, Bahia, Brazil aut NATIONALLICENCE 700 1- Fernandes Valéria Departamento de Ciências Biológicas, Centro de Microscopia Eletrônica, Universidade Estadual de Santa Cruz, Rodovia Ilhéus-Itabuna km 16, 45662-900, Ilhéus, Bahia, Brazil aut NATIONALLICENCE 700 1- Lucena Emerson Departamento de Ciências Biológicas, Centro de Microscopia Eletrônica, Universidade Estadual de Santa Cruz, Rodovia Ilhéus-Itabuna km 16, 45662-900, Ilhéus, Bahia, Brazil aut NATIONALLICENCE 700 1- Souza Vânia Departamento de Ciências Biológicas, Centro de Microscopia Eletrônica, Universidade Estadual de Santa Cruz, Rodovia Ilhéus-Itabuna km 16, 45662-900, Ilhéus, Bahia, Brazil aut NATIONALLICENCE 700 1- dos Santos Júnior Alberto Departamento de Ciências Biológicas, Centro de Microscopia Eletrônica, Universidade Estadual de Santa Cruz, Rodovia Ilhéus-Itabuna km 16, 45662-900, Ilhéus, Bahia, Brazil aut NATIONALLICENCE 700 1- Oliveira Arno Departamento de Ciências Biológicas, Centro de Microscopia Eletrônica, Universidade Estadual de Santa Cruz, Rodovia Ilhéus-Itabuna km 16, 45662-900, Ilhéus, Bahia, Brazil aut NATIONALLICENCE 700 1- Grenier-Loustalot Marie Service Central d'Analyse, CNRS, Echangeur de Solaize, Chemin du Canal, 69360, Solaize, France aut NATIONALLICENCE 700 1- Barbier Fréderique Service Central d'Analyse, CNRS, Echangeur de Solaize, Chemin du Canal, 69360, Solaize, France aut NATIONALLICENCE 700 1- Silva Delmira Departamento de Ciências Biológicas, Centro de Microscopia Eletrônica, Universidade Estadual de Santa Cruz, Rodovia Ilhéus-Itabuna km 16, 45662-900, Ilhéus, Bahia, Brazil aut NATIONALLICENCE 773 0- BioMetals Springer Netherlands 24/6(2011-12-01), 1017-1026 0966-0844 24:6<1017 2011 24 10534 Metadata rights reserved Springer special CC-BY-NC licence nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE 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