caa a22 4500 445321415 CHVBK 20180317142705.0 cr unu---uuuuu 170323e20111201xx s 000 0 eng 10.1007/s10534-011-9472-z doi (NATIONALLICENCE)springer-10.1007/s10534-011-9472-z Interaction of inorganic mercury with CoA-SH and acyl-CoAs [Elektronische Daten] [Robert Gradinaru, Alin Ionas, Aurel Pui, Gheorghita Zbancioc, Gabi Drochioiu] Sulfur containing biomolecules are involved in complexes with mercury. CoA is an important cofactor for many enzymes involved in metabolic processes. Fatty acyl-CoA-thioesters, substrates of mitochondrial ß-oxidation, are sulfur containing compounds and potential mercury ligands. The CoA-Hg2+ complex can be easily assessed by UV-Vis spectroscopy or indirectly by antibacterial tests that reconfirmed the protective role of CoA on E. coli. The characteristics of these complexes were determined by means of FTIR spectroscopy. The reverse phase liquid chromatography combined with electrospray ionization tandem mass spectrometry was used for detection of the side-product that resulted through the cleavage of thioesters in the presence of mercury. An unexpected result was the detection of octathioic acid as a product. Our study shows that mitochondrial β-oxidation can be affected by thioesters depletion assisted by Hg2+. The GC-MS technique could be used to detect some possible mitochondrial injuries due to the heavy metal ions. Springer Science+Business Media, LLC., 2011 Mercury nationallicence Coenzyme A nationallicence E. coli nationallicence Fatty acid thioesters nationallicence FTIR nationallicence GC-MS nationallicence Gradinaru Robert Department of Chemistry, Al. I. Cuza University, Ave. Carol I, No. 11, 700506, Jassy, Romania aut Ionas Alin Department of Chemistry, Al. I. Cuza University, Ave. Carol I, No. 11, 700506, Jassy, Romania aut Pui Aurel Department of Chemistry, Al. I. Cuza University, Ave. Carol I, No. 11, 700506, Jassy, Romania aut Zbancioc Gheorghita Department of Chemistry, Al. I. Cuza University, Ave. Carol I, No. 11, 700506, Jassy, Romania aut Drochioiu Gabi Department of Chemistry, Al. I. Cuza University, Ave. Carol I, No. 11, 700506, Jassy, Romania aut BioMetals Springer Netherlands 24/6(2011-12-01), 1115-1121 0966-0844 24:6<1115 2011 24 10534 https://doi.org/10.1007/s10534-011-9472-z text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1007/s10534-011-9472-z text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Gradinaru Robert Department of Chemistry, Al. I. Cuza University, Ave. Carol I, No. 11, 700506, Jassy, Romania aut NATIONALLICENCE 700 1- Ionas Alin Department of Chemistry, Al. I. Cuza University, Ave. Carol I, No. 11, 700506, Jassy, Romania aut NATIONALLICENCE 700 1- Pui Aurel Department of Chemistry, Al. I. Cuza University, Ave. Carol I, No. 11, 700506, Jassy, Romania aut NATIONALLICENCE 700 1- Zbancioc Gheorghita Department of Chemistry, Al. I. Cuza University, Ave. Carol I, No. 11, 700506, Jassy, Romania aut NATIONALLICENCE 700 1- Drochioiu Gabi Department of Chemistry, Al. I. Cuza University, Ave. Carol I, No. 11, 700506, Jassy, Romania aut NATIONALLICENCE 773 0- BioMetals Springer Netherlands 24/6(2011-12-01), 1115-1121 0966-0844 24:6<1115 2011 24 10534 Metadata rights reserved Springer special CC-BY-NC licence nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer