caa a22 4500 445321490 CHVBK 20180317142705.0 cr unu---uuuuu 170323e20110601xx s 000 0 eng 10.1007/s10534-010-9403-4 doi (NATIONALLICENCE)springer-10.1007/s10534-010-9403-4 A proteome analysis of the response of a Pseudomonas aeruginosa oxyR mutant to iron limitation [Elektronische Daten] [Tiffany Vinckx, Qing Wei, Sandra Matthijs, Jean-Paul Noben, Ruth Daniels, Pierre Cornelis] In Pseudomonas aeruginosa the response to oxidative stress is orchestrated by the LysR regulator OxyR by activation of the transcription of two catalase genes (katA and katB), of the alkyl-hydroxyperoxidases ahpCF and ahpB. Next to the expected high sensitivity to oxidative stress generated by reactive oxygen species (ROS: H2O2, O2 −), the oxyR mutant shows a defective growth under conditions of iron limitation (Vinckx et al. 2008). Although production and uptake of the siderophore pyoverdine is not affected by the absence of oxyR, the mutant is unable to satisfy its need for iron when grown under iron limiting conditions. In order to get a better insight into the effects caused by iron limitation on the physiological response of the oxyR mutant we decided to compare the proteomes of the wild type and the mutant grown in the iron-poor casamino acids medium (CAA), in CAA plus H2O2, and in CAA plus the strong iron chelator ethylenediamine-N,N′-bis(2-hydroxyphenylacetic acid) (EDDHA). Especially in the presence of hydrogen peroxide the oxyR cells increase the production of stress proteins (Dps and IbpA). The superoxide dismutase SodM is produced in higher amounts in the oxyR mutant grown in CAA plus H2O2. The PchB protein, a isochorismate-pyruvate lyase involved in the siderophore pyochelin biosynthesis is not detectable in the extracts from the oxyR mutant grown in the presence of hydrogen peroxide. When cells were grown in the presence of EDDHA, we observed a reduction of the ferric uptake regulator (Fur), and an increase in the two subunits of the succinyl-CoA synthetase and the fumarase FumC1. Springer Science+Business Media, LLC., 2011 Pseudomonas aeruginosa nationallicence oxyR nationallicence Oxidative stress nationallicence Iron limitation nationallicence Proteome nationallicence Vinckx Tiffany Laboratory of Microbial Interactions, Department of Molecular and Cellular Interactions, VIB, Vrije Universiteit Brussel, Building E, Room 6.6, Pleinlaan 2, 1050, Brussels, Belgium aut Wei Qing Laboratory of Microbial Interactions, Department of Molecular and Cellular Interactions, VIB, Vrije Universiteit Brussel, Building E, Room 6.6, Pleinlaan 2, 1050, Brussels, Belgium aut Matthijs Sandra Laboratory of Microbial Interactions, Department of Molecular and Cellular Interactions, VIB, Vrije Universiteit Brussel, Building E, Room 6.6, Pleinlaan 2, 1050, Brussels, Belgium aut Noben Jean-Paul Biomedical Research Institute, Hasselt University, 3590, Diepenbeek, Belgium aut Daniels Ruth Biomedical Research Institute, Hasselt University, 3590, Diepenbeek, Belgium aut Cornelis Pierre Laboratory of Microbial Interactions, Department of Molecular and Cellular Interactions, VIB, Vrije Universiteit Brussel, Building E, Room 6.6, Pleinlaan 2, 1050, Brussels, Belgium aut BioMetals Springer Netherlands 24/3(2011-06-01), 523-532 0966-0844 24:3<523 2011 24 10534 https://doi.org/10.1007/s10534-010-9403-4 text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1007/s10534-010-9403-4 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Vinckx Tiffany Laboratory of Microbial Interactions, Department of Molecular and Cellular Interactions, VIB, Vrije Universiteit Brussel, Building E, Room 6.6, Pleinlaan 2, 1050, Brussels, Belgium aut NATIONALLICENCE 700 1- Wei Qing Laboratory of Microbial Interactions, Department of Molecular and Cellular Interactions, VIB, Vrije Universiteit Brussel, Building E, Room 6.6, Pleinlaan 2, 1050, Brussels, Belgium aut NATIONALLICENCE 700 1- Matthijs Sandra Laboratory of Microbial Interactions, Department of Molecular and Cellular Interactions, VIB, Vrije Universiteit Brussel, Building E, Room 6.6, Pleinlaan 2, 1050, Brussels, Belgium aut NATIONALLICENCE 700 1- Noben Jean-Paul Biomedical Research Institute, Hasselt University, 3590, Diepenbeek, Belgium aut NATIONALLICENCE 700 1- Daniels Ruth Biomedical Research Institute, Hasselt University, 3590, Diepenbeek, Belgium aut NATIONALLICENCE 700 1- Cornelis Pierre Laboratory of Microbial Interactions, Department of Molecular and Cellular Interactions, VIB, Vrije Universiteit Brussel, Building E, Room 6.6, Pleinlaan 2, 1050, Brussels, Belgium aut NATIONALLICENCE 773 0- BioMetals Springer Netherlands 24/3(2011-06-01), 523-532 0966-0844 24:3<523 2011 24 10534 Metadata rights reserved Springer special CC-BY-NC licence nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer