caa a22 4500 445321520 CHVBK 20180317142705.0 cr unu---uuuuu 170323e20110601xx s 000 0 eng 10.1007/s10534-010-9393-2 doi (NATIONALLICENCE)springer-10.1007/s10534-010-9393-2 Bacterial gold sensing and resistance [Elektronische Daten] [Susana Checa, Fernando Soncini] Gold ions are mobilized and disseminated through the environment and enter into the cells by non-specific intake. To avoid deleterious effect that occurs even at very low concentrations, bacteria such as Salmonella enterica and Cupriavidus metallidurans use Au-specific MerR-type transcriptional regulators to detect the presence of these toxic ions, and control the expression of specific resistance factors. In contrast to the related copper sensor CueR, the Au-selective metalloregulatory proteins are able to distinguish Au(I) from Cu(I) or Ag(I). This is achieved by finely tuning a single dithiolate metal coordination with conserved cysteine residues at the metal binding site of the proteins to lower the affinity for Cu(I) in comparison to the Cu-sensors, while maintaining or even increasing the affinity for Au(I). In Salmonella, GolS not only privileges the binding of Au(I) over Cu(I) or Ag(I), but also distinguishes its target recognition sites in its regulated promoters minimizing cross-activation of CueR-controlled operators. In this sense, the presence of a selective Au sensory devise would allow species harbouring resident Cu-homeostasis systems to eliminate the toxic ion without affecting Cu acquisition in Au rich environments. Springer Science+Business Media, LLC., 2010 Gold sensing and resistance nationallicence MerR-regulators nationallicence Salmonella Typhimurium nationallicence Cupriavidus metallidurans nationallicence GolS nationallicence CueR nationallicence CupR nationallicence Checa Susana Instituto de Biología Molecular y Celular de Rosario, Departamento de Microbiología, Facultad de Ciencias Bioquímicas y Farmacéuticas, Universidad Nacional de Rosario, Consejo Nacional de Investigaciones Científicas y Técnicas. Suipacha 531, S2002LRK, Rosario, Argentina aut Soncini Fernando Instituto de Biología Molecular y Celular de Rosario, Departamento de Microbiología, Facultad de Ciencias Bioquímicas y Farmacéuticas, Universidad Nacional de Rosario, Consejo Nacional de Investigaciones Científicas y Técnicas. Suipacha 531, S2002LRK, Rosario, Argentina aut BioMetals Springer Netherlands 24/3(2011-06-01), 419-427 0966-0844 24:3<419 2011 24 10534 https://doi.org/10.1007/s10534-010-9393-2 text/html Onlinezugriff via DOI 1 review-article jats NATIONALLICENCE 856 40 https://doi.org/10.1007/s10534-010-9393-2 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Checa Susana Instituto de Biología Molecular y Celular de Rosario, Departamento de Microbiología, Facultad de Ciencias Bioquímicas y Farmacéuticas, Universidad Nacional de Rosario, Consejo Nacional de Investigaciones Científicas y Técnicas. Suipacha 531, S2002LRK, Rosario, Argentina aut NATIONALLICENCE 700 1- Soncini Fernando Instituto de Biología Molecular y Celular de Rosario, Departamento de Microbiología, Facultad de Ciencias Bioquímicas y Farmacéuticas, Universidad Nacional de Rosario, Consejo Nacional de Investigaciones Científicas y Técnicas. Suipacha 531, S2002LRK, Rosario, Argentina aut NATIONALLICENCE 773 0- BioMetals Springer Netherlands 24/3(2011-06-01), 419-427 0966-0844 24:3<419 2011 24 10534 Metadata rights reserved Springer special CC-BY-NC licence nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer