caa a22 4500 445321695 CHVBK 20180317142706.0 cr unu---uuuuu 170323e20110801xx s 000 0 eng 10.1007/s10534-011-9427-4 doi (NATIONALLICENCE)springer-10.1007/s10534-011-9427-4 Identification and characterization of a heme periplasmic-binding protein in Haemophilus ducreyi [Elektronische Daten] [Melissa St. Denis, Brigitte Sonier, Renée Robinson, Fraser Scott, D. Cameron, B. Lee] Haemophilus ducreyi, a gram-negative and heme-dependent bacterium, is the causative agent of chancroid, a genital ulcer sexually transmitted infection. Heme acquisition in H. ducreyi proceeds via a receptor mediated process in which the initial event involves binding of hemoglobin and heme to their cognate outer membrane proteins, HgbA and TdhA, respectively. Following this specific interaction, the fate of the periplasmic deposited heme is unclear. Using protein expression profiling of the H. ducreyi periplasmic proteome, a periplasmic-binding protein, termed hHbp, was identified whose expression was enhanced under heme-limited conditions. The gene encoding this protein was situated in a locus displaying genetic characteristics of an ABC transporter. The purified protein bound heme in a dose-dependent and saturable manner and this binding was specifically competitively inhibited by heme. The hhbp gene functionally complemented an Escherichia coli heme uptake mutant. Expression of the heme periplasmic-binding protein was detected in a limited survey of H. ducreyi and H. influenzae clinical strains. These results indicate that the passage of heme into the cytoplasm of H. ducreyi involves a heme dedicated ABC transporter. Springer Science+Business Media, LLC., 2011 Haemophilus ducreyi nationallicence Chancroid nationallicence Heme nationallicence ABC transporter nationallicence St. Denis Melissa Department of Biochemistry, Microbiology, and Immunology, Faculty of Medicine, University of Ottawa, 451 Smyth Road, K1H 8M5, Ottawa, ON, Canada aut Sonier Brigitte Department of Biochemistry, Microbiology, and Immunology, Faculty of Medicine, University of Ottawa, 451 Smyth Road, K1H 8M5, Ottawa, ON, Canada aut Robinson Renée Department of Biochemistry, Microbiology, and Immunology, Faculty of Medicine, University of Ottawa, 451 Smyth Road, K1H 8M5, Ottawa, ON, Canada aut Scott Fraser Department of Biochemistry, Microbiology, and Immunology, Faculty of Medicine, University of Ottawa, 451 Smyth Road, K1H 8M5, Ottawa, ON, Canada aut Cameron D. Department of Biochemistry, Microbiology, and Immunology, Faculty of Medicine, University of Ottawa, 451 Smyth Road, K1H 8M5, Ottawa, ON, Canada aut Lee B. Department of Biochemistry, Microbiology, and Immunology, Faculty of Medicine, University of Ottawa, 451 Smyth Road, K1H 8M5, Ottawa, ON, Canada aut BioMetals Springer Netherlands 24/4(2011-08-01), 709-722 0966-0844 24:4<709 2011 24 10534 https://doi.org/10.1007/s10534-011-9427-4 text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1007/s10534-011-9427-4 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- St. Denis Melissa Department of Biochemistry, Microbiology, and Immunology, Faculty of Medicine, University of Ottawa, 451 Smyth Road, K1H 8M5, Ottawa, ON, Canada aut NATIONALLICENCE 700 1- Sonier Brigitte Department of Biochemistry, Microbiology, and Immunology, Faculty of Medicine, University of Ottawa, 451 Smyth Road, K1H 8M5, Ottawa, ON, Canada aut NATIONALLICENCE 700 1- Robinson Renée Department of Biochemistry, Microbiology, and Immunology, Faculty of Medicine, University of Ottawa, 451 Smyth Road, K1H 8M5, Ottawa, ON, Canada aut NATIONALLICENCE 700 1- Scott Fraser Department of Biochemistry, Microbiology, and Immunology, Faculty of Medicine, University of Ottawa, 451 Smyth Road, K1H 8M5, Ottawa, ON, Canada aut NATIONALLICENCE 700 1- Cameron D. Department of Biochemistry, Microbiology, and Immunology, Faculty of Medicine, University of Ottawa, 451 Smyth Road, K1H 8M5, Ottawa, ON, Canada aut NATIONALLICENCE 700 1- Lee B. Department of Biochemistry, Microbiology, and Immunology, Faculty of Medicine, University of Ottawa, 451 Smyth Road, K1H 8M5, Ottawa, ON, Canada aut NATIONALLICENCE 773 0- BioMetals Springer Netherlands 24/4(2011-08-01), 709-722 0966-0844 24:4<709 2011 24 10534 Metadata rights reserved Springer special CC-BY-NC licence nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer