caa a22 4500 445321725 CHVBK 20180317142706.0 cr unu---uuuuu 170323e20110801xx s 000 0 eng 10.1007/s10534-011-9426-5 doi (NATIONALLICENCE)springer-10.1007/s10534-011-9426-5 Copper (II) complex of 1,10-phenanthroline and l -tyrosine with DNA oxidative cleavage activity in the gallic acid [Elektronische Daten] [Zhousheng Yang, Yong Wang, Geng Yang] Copper (II) complex of formulation [Cu-Phen-Tyr](H2O)](ClO4) (Phen=1,10-phenanthroline, l-Tyr=l-tyrosine), has been prepared, and their induced DNA oxidative cleavage activity studied. The complex binds to DNA by intercalation, as deduced from the absorption and fluorescence spectral data. Scatchard plots constructed from the absorption titration data gave binding constant 2.44×104M−1 of base pairs. Extensive hypochromism, broadening, and red shifts in the absorption spectra were observed. Upon binding to DNA, the fluorescence from the DNA-ethidium bromide system was efficiently quenched by the copper (II) complex. Stern-Volmer quenching constant 0.61×103M−1 obtained from the linear quenching plots. [Cu-Phen-Tyr] complex efficiently cleave the supercoiled DNA to its nicked circular form with gallic acid as biological reductant at appropriate complex concentration. The gallic acid as reductant could observably improve copper (II) complex to DNA damage. The pseudo-Michaelis-Menten kinetic parameters (k cat, K M) were calculated to be 1.32h−1 and 5.46×10−5M for [Cu-Phen-Tyr] complex. Mechanistic studies reveal the involvement of superoxide anions and hydroxyl radical (HO·) as the reactive species under an aerobic medium. Springer Science+Business Media, LLC., 2011 Copper (II) complexes nationallicence Gallic acid nationallicence DNA oxidative cleavage nationallicence Yang Zhousheng Anhui Key Laboratory of Chemo-Biosensing, College of Chemistry and Materials Science, Anhui Normal University, 1 Beijing Road, 241000, Wuhu, People's Republic of China aut Wang Yong Anhui Key Laboratory of Chemo-Biosensing, College of Chemistry and Materials Science, Anhui Normal University, 1 Beijing Road, 241000, Wuhu, People's Republic of China aut Yang Geng Anhui Key Laboratory of Chemo-Biosensing, College of Chemistry and Materials Science, Anhui Normal University, 1 Beijing Road, 241000, Wuhu, People's Republic of China aut BioMetals Springer Netherlands 24/4(2011-08-01), 737-745 0966-0844 24:4<737 2011 24 10534 https://doi.org/10.1007/s10534-011-9426-5 text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1007/s10534-011-9426-5 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Yang Zhousheng Anhui Key Laboratory of Chemo-Biosensing, College of Chemistry and Materials Science, Anhui Normal University, 1 Beijing Road, 241000, Wuhu, People's Republic of China aut NATIONALLICENCE 700 1- Wang Yong Anhui Key Laboratory of Chemo-Biosensing, College of Chemistry and Materials Science, Anhui Normal University, 1 Beijing Road, 241000, Wuhu, People's Republic of China aut NATIONALLICENCE 700 1- Yang Geng Anhui Key Laboratory of Chemo-Biosensing, College of Chemistry and Materials Science, Anhui Normal University, 1 Beijing Road, 241000, Wuhu, People's Republic of China aut NATIONALLICENCE 773 0- BioMetals Springer Netherlands 24/4(2011-08-01), 737-745 0966-0844 24:4<737 2011 24 10534 Metadata rights reserved Springer special CC-BY-NC licence nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer