caa a22 4500 445321768 CHVBK 20180317142706.0 cr unu---uuuuu 170323e20110801xx s 000 0 eng 10.1007/s10534-011-9423-8 doi (NATIONALLICENCE)springer-10.1007/s10534-011-9423-8 Lactoferrin: an iron-binding antimicrobial protein against Escherichia coli infection [Elektronische Daten] [Chih-Ching Yen, Chih-Jie Shen, Wu-Huei Hsu, Yi-Hsin Chang, Hsin-Tang Lin, Hsiao-Ling Chen, Chuan-Mu Chen] Escherichia coli (E. coli) are the most common aerobic gram-negative bacilli in a normal intestinal tract. They cause most of the intra-abdominal infections, wound infections associated with abdominal surgery, and septicemia. Most of these infections are of endogenous intestinal origin. Lactoferrin (LF) is an iron-binding glycoprotein found in milk and various external secretions. This protein has been found to have a number of biological functions, including antimicrobial, anti-cancer, antioxidant, and immunomodulatory effects. Partial degradation of LF by pepsin can give rise to peptides termed lactoferricin (LFcin) with more potent antimicrobial activity. LF and LFcin have been shown to inhibit the growth of a number of pathogenic bacteria (including E. coli and antibiotic-resistant strains), fungi, and even viruses in both in vitro and in vivo studies. We previously demonstrated that both recombinant porcine LF (pLF) produced from yeast and a synthetic 20-residue porcine LFcin peptide exhibit antimicrobial activity in vitro. In one of our recent studies, we performed pathogen challenges, including pathogenic E. coli, Staphylococcus aureus and Candida albicans, of the digestive tract of a transgenic milk-fed animal model. The results showed that LF has broad spectrum antimicrobial activity in the digestive tract and protects the mucosa of the small intestine from injury. Our following study also revealed that pLF as a feedstuff additive enhances avian immunity, including antibody formation and cell-mediated immunity. All of these results suggest that LF could be a novel natural protein in the treatment and prevention of infections with E. coli or antibiotic-resistant bacteria strains. Springer Science+Business Media, LLC., 2011 Lactoferrin nationallicence E. coli infection nationallicence Host defense protein nationallicence Sepsis nationallicence Antimicrobial activity nationallicence Nosocomial infection nationallicence Yen Chih-Ching Department of Life Sciences, National Chung Hsing University, No. 250, Kuo Kuang Rd, 402, Taichung, Taiwan aut Shen Chih-Jie Department of Life Sciences, National Chung Hsing University, No. 250, Kuo Kuang Rd, 402, Taichung, Taiwan aut Hsu Wu-Huei Department of Internal Medicine, China Medical University Hospital, Taichung, Taiwan aut Chang Yi-Hsin Department of Life Sciences, National Chung Hsing University, No. 250, Kuo Kuang Rd, 402, Taichung, Taiwan aut Lin Hsin-Tang Department of Food Science, Nutrition and Nutraceutical Biotechnology, Shih Chien University, Taipei, Taiwan aut Chen Hsiao-Ling Department of Molecular Biotechnology, Da-Yeh University, No.112, Shan-Jiau Road, Da-Tsuen, 515, Changhwa, Taiwan aut Chen Chuan-Mu Department of Life Sciences, National Chung Hsing University, No. 250, Kuo Kuang Rd, 402, Taichung, Taiwan aut BioMetals Springer Netherlands 24/4(2011-08-01), 585-594 0966-0844 24:4<585 2011 24 10534 https://doi.org/10.1007/s10534-011-9423-8 text/html Onlinezugriff via DOI 1 review-article jats NATIONALLICENCE 856 40 https://doi.org/10.1007/s10534-011-9423-8 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Yen Chih-Ching Department of Life Sciences, National Chung Hsing University, No. 250, Kuo Kuang Rd, 402, Taichung, Taiwan aut NATIONALLICENCE 700 1- Shen Chih-Jie Department of Life Sciences, National Chung Hsing University, No. 250, Kuo Kuang Rd, 402, Taichung, Taiwan aut NATIONALLICENCE 700 1- Hsu Wu-Huei Department of Internal Medicine, China Medical University Hospital, Taichung, Taiwan aut NATIONALLICENCE 700 1- Chang Yi-Hsin Department of Life Sciences, National Chung Hsing University, No. 250, Kuo Kuang Rd, 402, Taichung, Taiwan aut NATIONALLICENCE 700 1- Lin Hsin-Tang Department of Food Science, Nutrition and Nutraceutical Biotechnology, Shih Chien University, Taipei, Taiwan aut NATIONALLICENCE 700 1- Chen Hsiao-Ling Department of Molecular Biotechnology, Da-Yeh University, No.112, Shan-Jiau Road, Da-Tsuen, 515, Changhwa, Taiwan aut NATIONALLICENCE 700 1- Chen Chuan-Mu Department of Life Sciences, National Chung Hsing University, No. 250, Kuo Kuang Rd, 402, Taichung, Taiwan aut NATIONALLICENCE 773 0- BioMetals Springer Netherlands 24/4(2011-08-01), 585-594 0966-0844 24:4<585 2011 24 10534 Metadata rights reserved Springer special CC-BY-NC licence nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer