caa a22 4500 445321806 CHVBK 20180317142706.0 cr unu---uuuuu 170323e20110801xx s 000 0 eng 10.1007/s10534-011-9419-4 doi (NATIONALLICENCE)springer-10.1007/s10534-011-9419-4 Metabolomic profiles delineate potential roles for gadolinium chloride in the proliferation or inhibition of Hela cells [Elektronische Daten] [Xiao-Hui Long, Peng-Yuan Yang, Qiong Liu, Jun Yao, Yi Wang, Guo-Hua He, Guang-Yan Hong, Jia-Zuan Ni] Lanthanides (Lns) compounds have been reported to possess contrary effects on cell activity, i.e., promoting cell cycle progression and cell growth by lower concentration treatment, but suppressing cell proliferation and inducing cell apoptosis at higher dosing. However, the cellular processes during the intervention and the possible underlying mechanisms are still not well clarified. Using a combination of high-throughput liquid chromatography (LC) with mass spectrometry (MS), we have investigated the metabolomic profiles of Hela cells following gadolinium chloride (GdCl3) treatment in time- and concentration- dependent manners. A total of 48 metabolites released by Hela cells are identified to be differentially expressed (P<0.05) in different states. Metabolic pathways analyses reveal that the differential metabolites are mainly characterized by increased lipid and amino acid metabolisms and by decreased lipid, amino acid, and carbohydrate metabolisms for cells treated with GdCl3 at lower and higher concentrations, respectively. Notably, in the higher level GdCl3 case, the down-expressions of metabolites are predominantly in the glycolytic and the redox pathways. The above results, obtained by using a metabolomic strategy for the first time, disclose that different cell signaling pathways are activated by GdCl3 treatment with different concentrations, leading to inhibitory or promotional effect on Hela cells. Springer Science+Business Media, LLC., 2011 Gadolinium chloride nationallicence Hela cells nationallicence Metabolomic profiling nationallicence Liquid chromatography-mass spectrometry nationallicence Cell growth nationallicence Long Xiao-Hui College of Life Science, Shenzhen University, 518060, Guangdong, China aut Yang Peng-Yuan Institute for Biomedical Science, Fudan University, 200232, Shanghai, China aut Liu Qiong College of Life Science, Shenzhen University, 518060, Guangdong, China aut Yao Jun Institute for Biomedical Science, Fudan University, 200232, Shanghai, China aut Wang Yi Institute for Biomedical Science, Fudan University, 200232, Shanghai, China aut He Guo-Hua College of Life Science, Shenzhen University, 518060, Guangdong, China aut Hong Guang-Yan Key Laboratory of Rare Earth Chemistry and Physics, Changchun Institute of Applied Chemistry, Chinese Academy of Science, 130022, Jilin, China aut Ni Jia-Zuan Key Laboratory of Rare Earth Chemistry and Physics, Changchun Institute of Applied Chemistry, Chinese Academy of Science, 130022, Jilin, China aut BioMetals Springer Netherlands 24/4(2011-08-01), 663-677 0966-0844 24:4<663 2011 24 10534 https://doi.org/10.1007/s10534-011-9419-4 text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1007/s10534-011-9419-4 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Long Xiao-Hui College of Life Science, Shenzhen University, 518060, Guangdong, China aut NATIONALLICENCE 700 1- Yang Peng-Yuan Institute for Biomedical Science, Fudan University, 200232, Shanghai, China aut NATIONALLICENCE 700 1- Liu Qiong College of Life Science, Shenzhen University, 518060, Guangdong, China aut NATIONALLICENCE 700 1- Yao Jun Institute for Biomedical Science, Fudan University, 200232, Shanghai, China aut NATIONALLICENCE 700 1- Wang Yi Institute for Biomedical Science, Fudan University, 200232, Shanghai, China aut NATIONALLICENCE 700 1- He Guo-Hua College of Life Science, Shenzhen University, 518060, Guangdong, China aut NATIONALLICENCE 700 1- Hong Guang-Yan Key Laboratory of Rare Earth Chemistry and Physics, Changchun Institute of Applied Chemistry, Chinese Academy of Science, 130022, Jilin, China aut NATIONALLICENCE 700 1- Ni Jia-Zuan Key Laboratory of Rare Earth Chemistry and Physics, Changchun Institute of Applied Chemistry, Chinese Academy of Science, 130022, Jilin, China aut NATIONALLICENCE 773 0- BioMetals Springer Netherlands 24/4(2011-08-01), 663-677 0966-0844 24:4<663 2011 24 10534 Metadata rights reserved Springer special CC-BY-NC licence nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer