caa a22 4500 445321822 CHVBK 20180317142706.0 cr unu---uuuuu 170323e20111001xx s 000 0 eng 10.1007/s10534-011-9448-z doi (NATIONALLICENCE)springer-10.1007/s10534-011-9448-z Metallic ion content and damage to the DNA in oral mucosa cells of children with fixed orthodontic appliances [Elektronische Daten] [Esther Fernández-Miñano, Clara Ortiz, Ascensión Vicente, José Calvo, Antonio Ortiz] Although the metal devices used in orthodontic treatments are manufactured highly resistance to corrosion, they may still suffer some localized corrosion resulting from the oral cavity conditions. The corrosion causes the release of metals from the alloys used for their manufacture. In this report, we evaluated the in vivo metal ions release of three alloys (stainless steel, titanium and nickel-free) usually used in the orthodontics treatments and its genotoxicity. We applied to 15 patients, between 12 and 16years, 4 tubes and 20 brackets. Samples from oral mucosa were taken before the treatment and 30days later. The concentration of the titanium, chromium, manganese, cobalt, nickel, molybdenum and iron were detected using inductively coupled plasma mass spectrometry (ICP-MS). The genotoxicity was measured with a comet assay (Olive moment). The oral mucosa cells in contact with the stainless steel alloy displayed the greatest titanium and manganese concentrations and those in contact with the nickel-free alloy presented the greatest concentration of chromium and iron. Both alloys, stainless steel and nickel-free, induced a higher DNA damage in the oral mucosa cells than the titanium alloy, in which the Olive moment was similar to controls. Based on the results of our study, we can conclude that titanium brackets and tubes are the most biocompatible of the three alloys. Springer Science+Business Media, LLC., 2011 Orthodontic alloy nationallicence Metal toxicity nationallicence ICP-MS nationallicence DNA damage nationallicence Comet assay nationallicence Fernández-Miñano Esther Integral Pediatric Dentistry Teaching Unit, Faculty of Medicine, Dental Clinic, University of Murcia, Morales Meseguer Hospital, Avda. Marqués de los Vélez s/n, 30008, Murcia, Spain aut Ortiz Clara Department of Physiology, Faculty of Medicine, University of Murcia, 30100, Murcia, Spain aut Vicente Ascensión Docent Unit of Orthodontics, Faculty of Medicine, Dental Clinic, University of Murcia, 30008, Murcia, Spain aut Calvo José General Dentistry, Faculty of Medicine, Dental Clinic, University of Murcia, 30008, Murcia, Spain aut Ortiz Antonio Integral Pediatric Dentistry Teaching Unit, Faculty of Medicine, Dental Clinic, University of Murcia, Morales Meseguer Hospital, Avda. Marqués de los Vélez s/n, 30008, Murcia, Spain aut BioMetals Springer Netherlands 24/5(2011-10-01), 935-941 0966-0844 24:5<935 2011 24 10534 https://doi.org/10.1007/s10534-011-9448-z text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1007/s10534-011-9448-z text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Fernández-Miñano Esther Integral Pediatric Dentistry Teaching Unit, Faculty of Medicine, Dental Clinic, University of Murcia, Morales Meseguer Hospital, Avda. Marqués de los Vélez s/n, 30008, Murcia, Spain aut NATIONALLICENCE 700 1- Ortiz Clara Department of Physiology, Faculty of Medicine, University of Murcia, 30100, Murcia, Spain aut NATIONALLICENCE 700 1- Vicente Ascensión Docent Unit of Orthodontics, Faculty of Medicine, Dental Clinic, University of Murcia, 30008, Murcia, Spain aut NATIONALLICENCE 700 1- Calvo José General Dentistry, Faculty of Medicine, Dental Clinic, University of Murcia, 30008, Murcia, Spain aut NATIONALLICENCE 700 1- Ortiz Antonio Integral Pediatric Dentistry Teaching Unit, Faculty of Medicine, Dental Clinic, University of Murcia, Morales Meseguer Hospital, Avda. Marqués de los Vélez s/n, 30008, Murcia, Spain aut NATIONALLICENCE 773 0- BioMetals Springer Netherlands 24/5(2011-10-01), 935-941 0966-0844 24:5<935 2011 24 10534 Metadata rights reserved Springer special CC-BY-NC licence nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer