caa a22 4500 445321830 CHVBK 20180317142706.0 cr unu---uuuuu 170323e20111001xx s 000 0 eng 10.1007/s10534-011-9440-7 doi (NATIONALLICENCE)springer-10.1007/s10534-011-9440-7 Characterization of basolateral-to-apical transepithelial transport of cadmium in intestinal TC7 cell monolayers [Elektronische Daten] [Pascale Carrière, Marc Mantha, Sophie Champagne-Paradis, Catherine Jumarie] Cadmium (Cd) is a toxic metal with an extremely long half-life in humans. The intestinal absorption of Cd has been extensively studied but the role the intestinal epithelium may play in metal excretion has never been considered. The basolateral (BL)-to-apical (AP) transepithelial transport of Cd was characterized in TC7 human intestinal cells. Both AP and BL uptakes varied with days in culture, and BL uptake was twofold higher compared to AP in differentiated cultures. A 50% increase in the BL uptake of 0.5μM 109Cd was observed at pH 8.5 in a chloride but not nitrate medium, suggesting the involvement of a pH-sensitive mechanism of transport for chloro-complexes. Fe and Zn inhibited the BL uptake of Cd whereas complexation by albumin had no effect, but the stimulatory effect of pH 8.5 was lost in the presence of albumin. The BL uptake of [3H]-MPP+ and 109Cd were both inhibited by decynium22 without reciprocal inhibition. MRP2 and MDR1 mRNA levels increased as a function of days in culture. A 25 and 20% decrease in the cellular AP efflux of Cd was observed in the presence of verapamil and probenecid, respectively. In cells treated with BSO, which lowered by 26% the total cellular thiol content, the inhibitory effect of verapamil increased, whereas that of probenecid decreased. These results reveal the existence of a decynium22-sensitive mechanism of transport for Cd at the BL membrane, and suggest the involvement of MDR1 and MRP2 in cellular Cd efflux at the AP membrane. It is conceivable that the intestinal epithelium may contribute to Cd blood excretion. Springer Science+Business Media, LLC., 2011 Cadmium nationallicence Basolateral membrane nationallicence OCT nationallicence MRP2 nationallicence MDR1 nationallicence TC7 intestinal cells nationallicence Metal speciation nationallicence DMEM : Dulbecco's modified eagle essential minimum medium nationallicence FBS : Fetal bovine serum nationallicence MDR1 : Multidrug resistance nationallicence MPP+ : 1-Methyl-4-phenylpyridinium iodide nationallicence MRP : Multidrug resistance-associated protein nationallicence MTT : 3-[4,5-Dimethyl-2-thiazol-2-yl]-2,5-diphenyltetrazolium bromide nationallicence OCT : Organic cation transporter nationallicence TEER : Transepithelial electrical resistance nationallicence Carrière Pascale Département des sciences biologiques, Centre TOXEN, Université du Québec à Montréal, C.P. 8888, Succursale Centre-ville, H3C 3P8, Montréal, QC, Canada aut Mantha Marc Département des sciences biologiques, Centre TOXEN, Université du Québec à Montréal, C.P. 8888, Succursale Centre-ville, H3C 3P8, Montréal, QC, Canada aut Champagne-Paradis Sophie Département des sciences biologiques, Centre TOXEN, Université du Québec à Montréal, C.P. 8888, Succursale Centre-ville, H3C 3P8, Montréal, QC, Canada aut Jumarie Catherine Département des sciences biologiques, Centre TOXEN, Université du Québec à Montréal, C.P. 8888, Succursale Centre-ville, H3C 3P8, Montréal, QC, Canada aut BioMetals Springer Netherlands 24/5(2011-10-01), 857-874 0966-0844 24:5<857 2011 24 10534 https://doi.org/10.1007/s10534-011-9440-7 text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1007/s10534-011-9440-7 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Carrière Pascale Département des sciences biologiques, Centre TOXEN, Université du Québec à Montréal, C.P. 8888, Succursale Centre-ville, H3C 3P8, Montréal, QC, Canada aut NATIONALLICENCE 700 1- Mantha Marc Département des sciences biologiques, Centre TOXEN, Université du Québec à Montréal, C.P. 8888, Succursale Centre-ville, H3C 3P8, Montréal, QC, Canada aut NATIONALLICENCE 700 1- Champagne-Paradis Sophie Département des sciences biologiques, Centre TOXEN, Université du Québec à Montréal, C.P. 8888, Succursale Centre-ville, H3C 3P8, Montréal, QC, Canada aut NATIONALLICENCE 700 1- Jumarie Catherine Département des sciences biologiques, Centre TOXEN, Université du Québec à Montréal, C.P. 8888, Succursale Centre-ville, H3C 3P8, Montréal, QC, Canada aut NATIONALLICENCE 773 0- BioMetals Springer Netherlands 24/5(2011-10-01), 857-874 0966-0844 24:5<857 2011 24 10534 Metadata rights reserved Springer special CC-BY-NC licence nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer