caa a22 4500 44532192X CHVBK 20180317142706.0 cr unu---uuuuu 170323e20111001xx s 000 0 eng 10.1007/s10534-011-9444-3 doi (NATIONALLICENCE)springer-10.1007/s10534-011-9444-3 Some characteristics of membrane Cd2+ transport in rat thymocytes: an analysis using Fluo-3 [Elektronische Daten] [Takuya Kawanai, Masahiro Fujinaga, Kazuki Koizumi, Isao Kurotani, Erika Hashimoto, Masaya Satoh, Shoji Imai, Norikazu Miyoshi, Yasuo Oyama] Although cadmium-induced apoptosis of lymphocytes is one of common features in the immunotoxicity of cadmium, the membrane pathway for intracellular cadmium accumulation is not fully elucidated. To characterize membrane Cd2+ transport of rat thymocytes, the change in intracellular Cd2+ concentration under various conditions was examined by the use of Fluo-3, a fluorescent probe for monitoring the change in intracellular concentration of divalent metal cations. The membrane Cd2+ transport was estimated by the augmentation of Fluo-3 fluorescence induced by bath application of CdCl2. Lowering temperature strongly suppressed the augmentation of Fluo-3 fluorescence by CdCl2, suggesting that the metabolic process can be involved in membrane Cd2+ transport. External acidification (decreasing pH) and membrane depolarization by adding KCl attenuated the augmentation, indicating the requirement of electrochemical driving force for membrane Cd2+ transport into the cells. Bath application of CaCl2 and ZnCl2 equally decreased the augmentation, suggesting their competition with Cd2+ at the membrane transport. The augmentation by CdCl2 was lesser in the cells treated with N-ethylmaleimide inducing chemical depletion of cellular thiols. The result suggests the contribution of sulfhydryl groups to membrane Cd2+ transport. Taken together, it is suggested that the cells possess a temperature-sensitive membrane Cd2+ pathway, driven by electrochemical gradient of Cd2+ and transmembrane potential, with competitive binding site. Based on the characteristics described above, it is unlikely that the membrane Cd2+ transport in rat thymocytes is attributed to a single transport system although it has characteristics that are similar to those of divalent cation transporter 1. Springer Science+Business Media, LLC., 2011 Cadmium nationallicence Membrane nationallicence Transport nationallicence Flow cytometer nationallicence Fluo-3 nationallicence Kawanai Takuya Laboratories of Cellular Signaling, Environmental Biology, and Organometallic Chemistry, Faculty of Integrated Arts and Sciences, The University of Tokushima, 1-1 Minami-Jyosanjima, 770-8502, Tokushima, Japan aut Fujinaga Masahiro Laboratories of Cellular Signaling, Environmental Biology, and Organometallic Chemistry, Faculty of Integrated Arts and Sciences, The University of Tokushima, 1-1 Minami-Jyosanjima, 770-8502, Tokushima, Japan aut Koizumi Kazuki Laboratories of Cellular Signaling, Environmental Biology, and Organometallic Chemistry, Faculty of Integrated Arts and Sciences, The University of Tokushima, 1-1 Minami-Jyosanjima, 770-8502, Tokushima, Japan aut Kurotani Isao Laboratories of Cellular Signaling, Environmental Biology, and Organometallic Chemistry, Faculty of Integrated Arts and Sciences, The University of Tokushima, 1-1 Minami-Jyosanjima, 770-8502, Tokushima, Japan aut Hashimoto Erika Laboratories of Cellular Signaling, Environmental Biology, and Organometallic Chemistry, Faculty of Integrated Arts and Sciences, The University of Tokushima, 1-1 Minami-Jyosanjima, 770-8502, Tokushima, Japan aut Satoh Masaya Laboratories of Cellular Signaling, Environmental Biology, and Organometallic Chemistry, Faculty of Integrated Arts and Sciences, The University of Tokushima, 1-1 Minami-Jyosanjima, 770-8502, Tokushima, Japan aut Imai Shoji Laboratories of Cellular Signaling, Environmental Biology, and Organometallic Chemistry, Faculty of Integrated Arts and Sciences, The University of Tokushima, 1-1 Minami-Jyosanjima, 770-8502, Tokushima, Japan aut Miyoshi Norikazu Laboratories of Cellular Signaling, Environmental Biology, and Organometallic Chemistry, Faculty of Integrated Arts and Sciences, The University of Tokushima, 1-1 Minami-Jyosanjima, 770-8502, Tokushima, Japan aut Oyama Yasuo Laboratories of Cellular Signaling, Environmental Biology, and Organometallic Chemistry, Faculty of Integrated Arts and Sciences, The University of Tokushima, 1-1 Minami-Jyosanjima, 770-8502, Tokushima, Japan aut BioMetals Springer Netherlands 24/5(2011-10-01), 903-914 0966-0844 24:5<903 2011 24 10534 https://doi.org/10.1007/s10534-011-9444-3 text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1007/s10534-011-9444-3 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Kawanai Takuya Laboratories of Cellular Signaling, Environmental Biology, and Organometallic Chemistry, Faculty of Integrated Arts and Sciences, The University of Tokushima, 1-1 Minami-Jyosanjima, 770-8502, Tokushima, Japan aut NATIONALLICENCE 700 1- Fujinaga Masahiro Laboratories of Cellular Signaling, Environmental Biology, and Organometallic Chemistry, Faculty of Integrated Arts and Sciences, The University of Tokushima, 1-1 Minami-Jyosanjima, 770-8502, Tokushima, Japan aut NATIONALLICENCE 700 1- Koizumi Kazuki Laboratories of Cellular Signaling, Environmental Biology, and Organometallic Chemistry, Faculty of Integrated Arts and Sciences, The University of Tokushima, 1-1 Minami-Jyosanjima, 770-8502, Tokushima, Japan aut NATIONALLICENCE 700 1- Kurotani Isao Laboratories of Cellular Signaling, Environmental Biology, and Organometallic Chemistry, Faculty of Integrated Arts and Sciences, The University of Tokushima, 1-1 Minami-Jyosanjima, 770-8502, Tokushima, Japan aut NATIONALLICENCE 700 1- Hashimoto Erika Laboratories of Cellular Signaling, Environmental Biology, and Organometallic Chemistry, Faculty of Integrated Arts and Sciences, The University of Tokushima, 1-1 Minami-Jyosanjima, 770-8502, Tokushima, Japan aut NATIONALLICENCE 700 1- Satoh Masaya Laboratories of Cellular Signaling, Environmental Biology, and Organometallic Chemistry, Faculty of Integrated Arts and Sciences, The University of Tokushima, 1-1 Minami-Jyosanjima, 770-8502, Tokushima, Japan aut NATIONALLICENCE 700 1- Imai Shoji Laboratories of Cellular Signaling, Environmental Biology, and Organometallic Chemistry, Faculty of Integrated Arts and Sciences, The University of Tokushima, 1-1 Minami-Jyosanjima, 770-8502, Tokushima, Japan aut NATIONALLICENCE 700 1- Miyoshi Norikazu Laboratories of Cellular Signaling, Environmental Biology, and Organometallic Chemistry, Faculty of Integrated Arts and Sciences, The University of Tokushima, 1-1 Minami-Jyosanjima, 770-8502, Tokushima, Japan aut NATIONALLICENCE 700 1- Oyama Yasuo Laboratories of Cellular Signaling, Environmental Biology, and Organometallic Chemistry, Faculty of Integrated Arts and Sciences, The University of Tokushima, 1-1 Minami-Jyosanjima, 770-8502, Tokushima, Japan aut NATIONALLICENCE 773 0- BioMetals Springer Netherlands 24/5(2011-10-01), 903-914 0966-0844 24:5<903 2011 24 10534 Metadata rights reserved Springer special CC-BY-NC licence nationallicence BK010053 XK010053 XK010000 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