caa a22 4500 445321997 CHVBK 20180317142707.0 cr unu---uuuuu 170323e20110401xx s 000 0 eng 10.1007/s10534-010-9397-y doi (NATIONALLICENCE)springer-10.1007/s10534-010-9397-y Synechocystis ferredoxin-NADP+ oxidoreductase is capable of functioning as ferric reductase and of driving the Fenton reaction in the absence or presence of free flavin [Elektronische Daten] [Junichi Sato, Kouji Takeda, Rika Nishiyama, Toshihiro Watanabe, Mitsuru Abo, Etsuro Yoshimura, Junichi Nakagawa, Akira Abe, Shinji Kawasaki, Youichi Niimura] We purified free flavin-independent NADPH oxidoreductase from Synechocystis sp. PCC6803 based on NADPH oxidation activity elicited during reduction of t-butyl hydroperoxide in the presence of Fe(III)-EDTA. The N-terminal sequencing of the purified enzyme revealed it to be ferredoxin-NADP+ oxidoreductase (FNRS). The purified enzyme reacted with cytochrome c, ferricyanide and 2,6-dichloroindophenol (DCIP). The substrate specificity of the enzyme was similar to the known FNR. DNA degradation occurring in the presence of NADPH, Fe(III)-EDTA and hydrogen peroxide was potently enhanced by the purified enzyme, indicating that Synechocystis FNRS may drive the Fenton reaction. The Fenton reaction by Synechocystis FNRS in the presence of natural chelate iron compounds tended to be considerably lower than that in the presence of synthetic chelate iron compounds. The Synechocystis FNRS is considered to reduce ferric iron to ferrous iron when it evokes the Fenton reaction. Although Synechocystis FNRS was able to reduce iron compounds in the absence of free flavin, the ferric reduction by the enzyme was enhanced by the addition of free flavin. The enhancement was detected not only in the presence of natural chelate iron compounds but also synthetic chelate iron compounds. Springer Science+Business Media, LLC., 2011 Ferredoxin-NADP+ oxidoreductase nationallicence Ferric reductase nationallicence Fenton reaction nationallicence DCIP : 2,6-Dichloroindophenol nationallicence DTPA : Diethylenetriamine-N,N,N′,N″,N″-pentaacetic acid nationallicence FNR : Ferredoxin-NADP+ oxidoreductase nationallicence NTA : Nitrilotriacetic acid nationallicence Sato Junichi The Department of Bioscience, Tokyo University of Agriculture, 1-1-1 Sakuragaoka, 156-8502, Setagaya-ku, Tokyo, Japan aut Takeda Kouji The Department of Bioscience, Tokyo University of Agriculture, 1-1-1 Sakuragaoka, 156-8502, Setagaya-ku, Tokyo, Japan aut Nishiyama Rika The Department of Bioscience, Tokyo University of Agriculture, 1-1-1 Sakuragaoka, 156-8502, Setagaya-ku, Tokyo, Japan aut Watanabe Toshihiro Department of Food science and Technology, Tokyo University of Agriculture, Hokkaido, Japan aut Abo Mitsuru Department of Applied Biological Chemistry, Graduate School of Agricultural and Life Sciences, The University of Tokyo, Tokyo, Japan aut Yoshimura Etsuro Department of Applied Biological Chemistry, Graduate School of Agricultural and Life Sciences, The University of Tokyo, Tokyo, Japan aut Nakagawa Junichi Department of Food science and Technology, Tokyo University of Agriculture, Hokkaido, Japan aut Abe Akira Department of Internal Medicine, Nephrology Division, University of Michigan, Michigan, USA aut Kawasaki Shinji The Department of Bioscience, Tokyo University of Agriculture, 1-1-1 Sakuragaoka, 156-8502, Setagaya-ku, Tokyo, Japan aut Niimura Youichi The Department of Bioscience, Tokyo University of Agriculture, 1-1-1 Sakuragaoka, 156-8502, Setagaya-ku, Tokyo, Japan aut BioMetals Springer Netherlands 24/2(2011-04-01), 311-321 0966-0844 24:2<311 2011 24 10534 https://doi.org/10.1007/s10534-010-9397-y text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1007/s10534-010-9397-y text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Sato Junichi The Department of Bioscience, Tokyo University of Agriculture, 1-1-1 Sakuragaoka, 156-8502, Setagaya-ku, Tokyo, Japan aut NATIONALLICENCE 700 1- Takeda Kouji The Department of Bioscience, Tokyo University of Agriculture, 1-1-1 Sakuragaoka, 156-8502, Setagaya-ku, Tokyo, Japan aut NATIONALLICENCE 700 1- Nishiyama Rika The Department of Bioscience, Tokyo University of Agriculture, 1-1-1 Sakuragaoka, 156-8502, Setagaya-ku, Tokyo, Japan aut NATIONALLICENCE 700 1- Watanabe Toshihiro Department of Food science and Technology, Tokyo University of Agriculture, Hokkaido, Japan aut NATIONALLICENCE 700 1- Abo Mitsuru Department of Applied Biological Chemistry, Graduate School of Agricultural and Life Sciences, The University of Tokyo, Tokyo, Japan aut NATIONALLICENCE 700 1- Yoshimura Etsuro Department of Applied Biological Chemistry, Graduate School of Agricultural and Life Sciences, The University of Tokyo, Tokyo, Japan aut NATIONALLICENCE 700 1- Nakagawa Junichi Department of Food science and Technology, Tokyo University of Agriculture, Hokkaido, Japan aut NATIONALLICENCE 700 1- Abe Akira Department of Internal Medicine, Nephrology Division, University of Michigan, Michigan, USA aut NATIONALLICENCE 700 1- Kawasaki Shinji The Department of Bioscience, Tokyo University of Agriculture, 1-1-1 Sakuragaoka, 156-8502, Setagaya-ku, Tokyo, Japan aut NATIONALLICENCE 700 1- Niimura Youichi The Department of Bioscience, Tokyo University of Agriculture, 1-1-1 Sakuragaoka, 156-8502, Setagaya-ku, Tokyo, Japan aut NATIONALLICENCE 773 0- BioMetals Springer Netherlands 24/2(2011-04-01), 311-321 0966-0844 24:2<311 2011 24 10534 Metadata rights reserved Springer special CC-BY-NC licence nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer