caa a22 4500 445322012 CHVBK 20180317142707.0 cr unu---uuuuu 170323e20110401xx s 000 0 eng 10.1007/s10534-010-9405-2 doi (NATIONALLICENCE)springer-10.1007/s10534-010-9405-2 Heterogenous distribution of ferroportin-containing neurons in mouse brain [Elektronische Daten] [Michael Boserup, Jacek Lichota, David Haile, Torben Moos] Iron is crucial for a variety of cellular functions in neuronal cells. Neuronal iron uptake is reflected in a robust and consistent expression of transferrin receptors and divalent metal transporter 1 (DMT 1). Conversely, the mechanisms by which neurons neutralize and possibly excrete iron are less clear. Studies indicate that neurons express ferroportin which could reflect a mechanism for iron export. We mapped the distribution of ferroportin in the adult mouse brain using an antibody prepared from a peptide representing amino acid sequences 223-303 of mouse ferroportin. The antibody specifically detected ferroportin in brain homogenates, whereas homogenates of cultured endothelial cells were devoid of immunoreactivity. In brain sections, ferroportin was confined to neuronal cell bodies and peripheral processes of cerebral cortex, hippocampus, thalamus, brain stem, and cerebellum. In brain stem ferroportin-labeling was particularly high in neurons of cranial nerve nuclei and reticular formation. Ferroportin was hardly detectable in striatum, pallidum, or hypothalamus. Among non-neuronal cells, ferroportin was detected in oligodendrocytes and choroid plexus epithelial cells. A comparison with previous studies on the distribution of transferrin receptors in neurons shows that many neuronal pools coincide with those expressing ferroportin. The data therefore indicate that neuronal iron homeostasis consists of a delicate balance between transferrin receptor-mediated uptake of iron-transferrin and ferroportin-related iron excretion. The findings also suggest a particular high turnover of iron in neuronal regions, such as habenula, hippocampus, reticular formation and cerebellum, as several neurons in these regions exhibit a prominent co-expression of transferrin receptors and ferroportin. Springer Science+Business Media, LLC., 2011 Blood-brain barrier nationallicence Iron nationallicence Neurodegeneration nationallicence Oxidative stress nationallicence qPCR nationallicence Transferrin nationallicence 3N : Oculomotor nucleus nationallicence 3V : 3rd ventricle nationallicence 5N : Motor trigeminal nucleus nationallicence 7N : Facial nucleus nationallicence M1 : Primary motor cortex nationallicence Amb : Ambiguus nucleus nationallicence bEnd3 : Mouse brain endothelial cells nationallicence CA1 : Field CA1 of Ammon's horn nationallicence CA2 : Field CA2 of Ammon's horn nationallicence CA3 : Field CA3 of Ammon's horn nationallicence cc : Corpus callosum nationallicence CP : Choroid plexus nationallicence D3V : Dorsal part of the third ventricle nationallicence DAB : 3,3-Diaminobenzidine tetrahydrochloride nationallicence DG : Dentate gyrus nationallicence DMT 1 : Divalent metal transporter 1 nationallicence DR : Dorsal raphe nucleus nationallicence EPl : External plexiform layer of the olfactory bulb nationallicence EPlA : External plexiform layer of the accessory olfactory bulb nationallicence Fpn-ir : Ferroportin-immunoreactivity nationallicence Gl : Glomerular layer of the olfactory bulb nationallicence GrO : Granular cell layer of the olfactory bulb nationallicence hbc : Habenular commissure nationallicence hi : Habenulo-interpeduncular tract nationallicence IntP : Interposed cerebellar nucleus posterior part nationallicence IG : Induseum griseum nationallicence IP : Interpeduncular nucleus nationallicence IPl : Internal plexiform layer of the olfactory bulb nationallicence Lat : Lateral cerebellar nucleus nationallicence LC : Locus coeruleus nationallicence LHb : Lateral habenular nucleus nationallicence ME : Median eminence nationallicence Med : Medial cerebellar nucleus nationallicence MHb : Medial habenular nucleus nationallicence Mi : Mitral cell layer of the olfactory bulb nationallicence Pir : Piriform cortex nationallicence PPBS : Potassium phosphate-buffered saline nationallicence Rbd : Rhabdoid nucleus nationallicence RT : Reverse transcriptase nationallicence TSA : Tyramid signal amplification nationallicence UTR : Untranslated region nationallicence VM : Ventral medial thalamic nucleus nationallicence Boserup Michael Section of Neurobiology, Biomedicine, Department of Health Science and Technology, Aalborg University, Fr. Bajers Vej 3B, 1.216, 9220, Aalborg East, Denmark aut Lichota Jacek Section of Neurobiology, Biomedicine, Department of Health Science and Technology, Aalborg University, Fr. Bajers Vej 3B, 1.216, 9220, Aalborg East, Denmark aut Haile David South Texas Veterans Health Care System, San Antonio, TX, USA aut Moos Torben Section of Neurobiology, Biomedicine, Department of Health Science and Technology, Aalborg University, Fr. Bajers Vej 3B, 1.216, 9220, Aalborg East, Denmark aut BioMetals Springer Netherlands 24/2(2011-04-01), 357-375 0966-0844 24:2<357 2011 24 10534 https://doi.org/10.1007/s10534-010-9405-2 text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1007/s10534-010-9405-2 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Boserup Michael Section of Neurobiology, Biomedicine, Department of Health Science and Technology, Aalborg University, Fr. Bajers Vej 3B, 1.216, 9220, Aalborg East, Denmark aut NATIONALLICENCE 700 1- Lichota Jacek Section of Neurobiology, Biomedicine, Department of Health Science and Technology, Aalborg University, Fr. Bajers Vej 3B, 1.216, 9220, Aalborg East, Denmark aut NATIONALLICENCE 700 1- Haile David South Texas Veterans Health Care System, San Antonio, TX, USA aut NATIONALLICENCE 700 1- Moos Torben Section of Neurobiology, Biomedicine, Department of Health Science and Technology, Aalborg University, Fr. Bajers Vej 3B, 1.216, 9220, Aalborg East, Denmark aut NATIONALLICENCE 773 0- BioMetals Springer Netherlands 24/2(2011-04-01), 357-375 0966-0844 24:2<357 2011 24 10534 Metadata rights reserved Springer special CC-BY-NC licence nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer