caa a22 4500 445322020 CHVBK 20180317142707.0 cr unu---uuuuu 170323e20110401xx s 000 0 eng 10.1007/s10534-010-9385-2 doi (NATIONALLICENCE)springer-10.1007/s10534-010-9385-2 TonB-dependent outer-membrane proteins and siderophore utilization in Pseudomonas fluorescens Pf-5 [Elektronische Daten] [Sierra Hartney, Sylvie Mazurier, Teresa Kidarsa, Maria Quecine, Philippe Lemanceau, Joyce Loper] The soil bacterium Pseudomonas fluorescens Pf-5 produces two siderophores, a pyoverdine and enantio-pyochelin, and its proteome includes 45 TonB-dependent outer-membrane proteins, which commonly function in uptake of siderophores and other substrates from the environment. The 45 proteins share the conserved β-barrel and plug domains of TonB-dependent proteins but only 18 of them have an N-terminal signaling domain characteristic of TonB-dependent transducers (TBDTs), which participate in cell-surface signaling systems. Phylogenetic analyses of the 18 TBDTs and 27 TonB-dependent receptors (TBDRs), which lack the N-terminal signaling domain, suggest a complex evolutionary history including horizontal transfer among different microbial lineages. Putative functions were assigned to certain TBDRs and TBDTs in clades including well-characterized orthologs from other Pseudomonas spp. A mutant of Pf-5 with deletions in pyoverdine and enantio-pyochelin biosynthesis genes was constructed and characterized for iron-limited growth and utilization of a spectrum of siderophores. The mutant could utilize as iron sources a large number of pyoverdines with diverse structures as well as ferric citrate, heme, and the siderophores ferrichrome, ferrioxamine B, enterobactin, and aerobactin. The diversity and complexity of the TBDTs and TBDRs with roles in iron uptake clearly indicate the importance of iron in the fitness and survival of Pf-5 in the environment. Springer Science+Business Media, LLC (outside the USA), 2010 Pyoverdine nationallicence Iron-acquisition nationallicence TonB-dependent receptors nationallicence Pseudomonas fluorescens nationallicence Hartney Sierra Department of Botany and Plant Pathology, Oregon State University, 2082 Cordley Hall, 97331, Corvallis, OR, USA aut Mazurier Sylvie INRA, Université de Bourgogne, UMR1229 ‘Microbiologie du Sol et de l'Environement', CMSE, BV 86510, 21034, Dijon Cedex, France aut Kidarsa Teresa Horticultural Crops Research Laboratory, United States Department of Agriculture, Agricultural Research Service, 3420 N.W. Orchard Avenue, 97330, Corvallis, OR, USA aut Quecine Maria Department of Genetics, Escola Superior de Agricultura ‘‘Luiz de Queiroz'', University of São Paulo, 11 Pádua Dias Avenue, Piracicaba, SP, Brazil aut Lemanceau Philippe INRA, Université de Bourgogne, UMR1229 ‘Microbiologie du Sol et de l'Environement', CMSE, BV 86510, 21034, Dijon Cedex, France aut Loper Joyce Horticultural Crops Research Laboratory, United States Department of Agriculture, Agricultural Research Service, 3420 N.W. Orchard Avenue, 97330, Corvallis, OR, USA aut BioMetals Springer Netherlands 24/2(2011-04-01), 193-213 0966-0844 24:2<193 2011 24 10534 https://doi.org/10.1007/s10534-010-9385-2 text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1007/s10534-010-9385-2 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Hartney Sierra Department of Botany and Plant Pathology, Oregon State University, 2082 Cordley Hall, 97331, Corvallis, OR, USA aut NATIONALLICENCE 700 1- Mazurier Sylvie INRA, Université de Bourgogne, UMR1229 ‘Microbiologie du Sol et de l'Environement', CMSE, BV 86510, 21034, Dijon Cedex, France aut NATIONALLICENCE 700 1- Kidarsa Teresa Horticultural Crops Research Laboratory, United States Department of Agriculture, Agricultural Research Service, 3420 N.W. Orchard Avenue, 97330, Corvallis, OR, USA aut NATIONALLICENCE 700 1- Quecine Maria Department of Genetics, Escola Superior de Agricultura ‘‘Luiz de Queiroz'', University of São Paulo, 11 Pádua Dias Avenue, Piracicaba, SP, Brazil aut NATIONALLICENCE 700 1- Lemanceau Philippe INRA, Université de Bourgogne, UMR1229 ‘Microbiologie du Sol et de l'Environement', CMSE, BV 86510, 21034, Dijon Cedex, France aut NATIONALLICENCE 700 1- Loper Joyce Horticultural Crops Research Laboratory, United States Department of Agriculture, Agricultural Research Service, 3420 N.W. Orchard Avenue, 97330, Corvallis, OR, USA aut NATIONALLICENCE 773 0- BioMetals Springer Netherlands 24/2(2011-04-01), 193-213 0966-0844 24:2<193 2011 24 10534 Metadata rights reserved Springer special CC-BY-NC licence nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer