caa a22 4500 445322047 CHVBK 20180317142707.0 cr unu---uuuuu 170323e20110401xx s 000 0 eng 10.1007/s10534-010-9386-1 doi (NATIONALLICENCE)springer-10.1007/s10534-010-9386-1 Heme oxygenase is involved in cobalt chloride-induced lateral root development in tomato [Elektronische Daten] [Sheng Xu, Bo Zhang, Ze-Yu Cao, Teng-Fang Ling, Wen-Biao Shen] In animals, heme oxygenase (HO), a rate-limiting enzyme responsible for carbon monoxide (CO) production, was regarded as a protective system maintaining cellular homeostasis. It was also established that metal ions are powerful HO-inducing agents and cobalt chloride (CoCl2) was the first metal ion identified with an inducing property. Previous study suggests that CoCl2 stimulates adventitious root formation in tomato and cucumber cuttings. In this test, we discover that both CoCl2 and an inducer of HO-1, hemin, could lead to the promotion of lateral root development, as well as the induction of HO-1 protein expression, HO activity, or LeHO-1/2 transcripts, in lateral root initiation zone of tomato seedlings. The effect is specific for HO since the potent HO-1 inhibitor zinc protoporphyrin IX (ZnPPIX) blocked the above actions of CoCl2, and the inhibitory effect was reversed partially when 50% CO aqueous solution was added. However, the addition of ascorbic acid (AsA), a well-known antioxidant, exhibited no obvious effect on lateral root formation. Molecular evidence further showed that CoCl2-induced the up-regulation of target genes responsible for lateral root formation, including LeCDKA1, LeCYCA2;1, and LeCYCA3;1, was suppressed differentially by ZnPPIX. And these decreases were reversed further by the addition of CO. All together, these results suggest a novel role for HO in the CoCl2-induced tomato lateral root formation. Springer Science+Business Media, LLC., 2010 Cell cycle regulatory gene nationallicence Cobalt chloride nationallicence Heme oxygenase nationallicence Lateral root development nationallicence Lycopersicon esculentum nationallicence Xu Sheng Department of Biochemistry and Molecular Biology, College of Life Sciences, Nanjing Agricultural University, 210095, Nanjing, People's Republic of China aut Zhang Bo Department of Biochemistry and Molecular Biology, College of Life Sciences, Nanjing Agricultural University, 210095, Nanjing, People's Republic of China aut Cao Ze-Yu Department of Biochemistry and Molecular Biology, College of Life Sciences, Nanjing Agricultural University, 210095, Nanjing, People's Republic of China aut Ling Teng-Fang Department of Biochemistry and Molecular Biology, College of Life Sciences, Nanjing Agricultural University, 210095, Nanjing, People's Republic of China aut Shen Wen-Biao Department of Biochemistry and Molecular Biology, College of Life Sciences, Nanjing Agricultural University, 210095, Nanjing, People's Republic of China aut BioMetals Springer Netherlands 24/2(2011-04-01), 181-191 0966-0844 24:2<181 2011 24 10534 https://doi.org/10.1007/s10534-010-9386-1 text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1007/s10534-010-9386-1 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Xu Sheng Department of Biochemistry and Molecular Biology, College of Life Sciences, Nanjing Agricultural University, 210095, Nanjing, People's Republic of China aut NATIONALLICENCE 700 1- Zhang Bo Department of Biochemistry and Molecular Biology, College of Life Sciences, Nanjing Agricultural University, 210095, Nanjing, People's Republic of China aut NATIONALLICENCE 700 1- Cao Ze-Yu Department of Biochemistry and Molecular Biology, College of Life Sciences, Nanjing Agricultural University, 210095, Nanjing, People's Republic of China aut NATIONALLICENCE 700 1- Ling Teng-Fang Department of Biochemistry and Molecular Biology, College of Life Sciences, Nanjing Agricultural University, 210095, Nanjing, People's Republic of China aut NATIONALLICENCE 700 1- Shen Wen-Biao Department of Biochemistry and Molecular Biology, College of Life Sciences, Nanjing Agricultural University, 210095, Nanjing, People's Republic of China aut NATIONALLICENCE 773 0- BioMetals Springer Netherlands 24/2(2011-04-01), 181-191 0966-0844 24:2<181 2011 24 10534 Metadata rights reserved Springer special CC-BY-NC licence nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer