caa a22 4500 445322055 CHVBK 20180317142707.0 cr unu---uuuuu 170323e20110401xx s 000 0 eng 10.1007/s10534-010-9396-z doi (NATIONALLICENCE)springer-10.1007/s10534-010-9396-z Bafana Amit Biotechnology Division, Institute of Himalayan Bioresource Technology (IHBT), Council of Scientific and Industrial Research (CSIR), 176061, Palampur, Himachal Pradesh, India aut Mercury resistance in Sporosarcina sp. G3 [Elektronische Daten] [Amit Bafana] Mercuric reductase (MerA) enzyme plays an important role in biogeochemical cycling and detoxification of Hg and recently, has also been shown to be useful in clean up of Hg-contaminated effluents. Present study describes isolation of a heavy metal-resistant isolate of Sporosarcina, which could tolerate up to 40, 525, 210, 2900 and 370μM of Cd, Co, Zn, Cr and Hg respectively. It was found to reduce and detoxify redox-active metals like Cr and Hg. The chromate reductase and MerA activities in the crude cell extract of the culture were 1.5 and 0.044units/mg protein respectively. The study also describes designing of a new set of highly degenerate primers based on a dataset of 23 Firmicute merA genes. As the primers encompass the known diversity of merA genes within the phylum Firmicutes, they can be very useful for functional diversity analysis. They were successfully used to amplify a 787bp merA fragment from the current isolate. A 1174bp merA fragment was further cloned by designing an additional downstream primer. It was found to show 92% similarity to the putative merA gene from Bacillus cereus AH820. To the best of our knowledge, this is the first report of mercury resistance and merA gene sequence from Sporosarcina. Springer Science+Business Media, LLC., 2010 Mercuric reductase nationallicence merA nationallicence Firmicutes nationallicence Gram positive nationallicence Degenerate primer nationallicence BioMetals Springer Netherlands 24/2(2011-04-01), 301-309 0966-0844 24:2<301 2011 24 10534 https://doi.org/10.1007/s10534-010-9396-z text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1007/s10534-010-9396-z text/html Onlinezugriff via DOI NATIONALLICENCE 100 1- Bafana Amit Biotechnology Division, Institute of Himalayan Bioresource Technology (IHBT), Council of Scientific and Industrial Research (CSIR), 176061, Palampur, Himachal Pradesh, India aut NATIONALLICENCE 773 0- BioMetals Springer Netherlands 24/2(2011-04-01), 301-309 0966-0844 24:2<301 2011 24 10534 Metadata rights reserved Springer special CC-BY-NC licence nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer