caa a22 4500 445322071 CHVBK 20180317142707.0 cr unu---uuuuu 170323e20110401xx s 000 0 eng 10.1007/s10534-010-9395-0 doi (NATIONALLICENCE)springer-10.1007/s10534-010-9395-0 Selenoprotein W gene expression in the gastrointestinal tract of chicken is affected by dietary selenium [Elektronische Daten] [Jin-Long Li, Hui-Xin Li, Shu Li, Zhi-Hui Jiang, Shi-Wen Xu, Zhao-Xin Tang] Selenoprotein W (SelW) and selenium (Se) plays important roles in gastrointestinal function and that SelW expression in the gastrointestinal system of mammals is sensitive to Se levels. However, little is known about the pattern of SelW expression in the bird gastrointestinal tract. To investigate the distribution of SelW and effects of dietary Se levels on the SelW mRNA expression in the gastrointestinal tract tissues of birds, 1-day-old male chickens were fed either a commercial diet or a Se-supplemented diet containing 1.0, 2.0, 3.0 or 5.0mg/kg sodium selenite for 90days. The gastrointestinal tract tissues (tongue, esophagus, crop, proventriculus, gizzard, duodenum, small intestine, cecum and rectum) were collected and examined for Se content and mRNA levels of SelW. The mRNA expression of SelW was detected in all tissues. The greatest increase in SelW mRNA levels was observed in the gizzard, whereas Se content was highest in the duodenum and small intestine. A significant increase in SelW mRNA levels was observed in the gastrointestinal tract tissues of chickens fed the diets containing 1-3mg/kg sodium selenite while decreased SelW mRNA levels were observed in the esophagus, crop, proventriculus, gizzard, duodenum and cecum in chickens fed the diet containing 5mg/kg sodium selenite. These data indicate that SelW is widely expressed in the gastrointestinal tract tissues of birds and the transcription of the SelW gene is very sensitive to dietary Se. Springer Science+Business Media, LLC., 2010 Chicken nationallicence Selenoprotein W nationallicence Selenium nationallicence mRNA expression nationallicence Gastrointestinal tract tissues nationallicence Li Jin-Long College of Veterinary Medicine, Northeast Agricultural University, 150030, Harbin, People's Republic of China aut Li Hui-Xin Division of Avian Infectious Diseases, State Key Laboratory of Veterinary Biotechnology, Harbin Veterinary Research Institute, Chinese Academy of Agricultural Sciences, 150001, Harbin, People's Republic of China aut Li Shu College of Veterinary Medicine, Northeast Agricultural University, 150030, Harbin, People's Republic of China aut Jiang Zhi-Hui College of Veterinary Medicine, Northeast Agricultural University, 150030, Harbin, People's Republic of China aut Xu Shi-Wen College of Veterinary Medicine, Northeast Agricultural University, 150030, Harbin, People's Republic of China aut Tang Zhao-Xin College of Veterinary Medicine, South China Agricultural University, 510640, Guangzhou, People's Republic of China aut BioMetals Springer Netherlands 24/2(2011-04-01), 291-299 0966-0844 24:2<291 2011 24 10534 https://doi.org/10.1007/s10534-010-9395-0 text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1007/s10534-010-9395-0 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Li Jin-Long College of Veterinary Medicine, Northeast Agricultural University, 150030, Harbin, People's Republic of China aut NATIONALLICENCE 700 1- Li Hui-Xin Division of Avian Infectious Diseases, State Key Laboratory of Veterinary Biotechnology, Harbin Veterinary Research Institute, Chinese Academy of Agricultural Sciences, 150001, Harbin, People's Republic of China aut NATIONALLICENCE 700 1- Li Shu College of Veterinary Medicine, Northeast Agricultural University, 150030, Harbin, People's Republic of China aut NATIONALLICENCE 700 1- Jiang Zhi-Hui College of Veterinary Medicine, Northeast Agricultural University, 150030, Harbin, People's Republic of China aut NATIONALLICENCE 700 1- Xu Shi-Wen College of Veterinary Medicine, Northeast Agricultural University, 150030, Harbin, People's Republic of China aut NATIONALLICENCE 700 1- Tang Zhao-Xin College of Veterinary Medicine, South China Agricultural University, 510640, Guangzhou, People's Republic of China aut NATIONALLICENCE 773 0- BioMetals Springer Netherlands 24/2(2011-04-01), 291-299 0966-0844 24:2<291 2011 24 10534 Metadata rights reserved Springer special CC-BY-NC licence nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer