caa a22 4500 44532208X CHVBK 20180317142707.0 cr unu---uuuuu 170323e20110401xx s 000 0 eng 10.1007/s10534-010-9388-z doi (NATIONALLICENCE)springer-10.1007/s10534-010-9388-z A role for ferritin in the antioxidant system in coffee cell cultures [Elektronische Daten] [Alexandra Bottcher, Paula Nobile, Paula Martins, Fábio Conte, Ricardo Azevedo, Paulo Mazzafera] Iron (Fe) is an essential nutrient for plants, but it can generate oxidative stress at high concentrations. In this study, Coffea arabica L. cell suspension cultures were exposed to excess Fe (60 and 240μM) to investigate changes in the gene expression of ferritin and antioxidant enzymes. Iron content accumulated during cell growth, and Western blot analysis showed an increase of ferritin in cells treated with Fe. The expression of two ferritin genes retrieved from the Brazilian coffee EST database was studied. CaFER1, but not CaFER2, transcripts were induced by Fe exposure. Phylogenetic analysis revealed that CaFER1 is not similar to CaFER2 or to any ferritin that has been characterised in detail. The increase in ferritin gene expression was accompanied by an increase in the activity of antioxidant enzymes. Superoxide dismutase, guaiacol peroxidase, catalase, and glutathione reductase activities increased in cells grown in the presence of excess Fe, especially at 60μM, while the activity of glutathione S-transferase decreased. These data suggest that Fe induces oxidative stress in coffee cell suspension cultures and that ferritin participates in the antioxidant system to protect cells against oxidative damage. Thus, cellular Fe concentrations must be finely regulated to avoid cellular damage most likely caused by increased oxidative stress induced by Fe. However, transcriptional analyses indicate that ferritin genes are differentially controlled, as only CaFER1 expression was responsive to Fe treatment. Springer Science+Business Media, LLC., 2010 Coffea arabica nationallicence Ferritin nationallicence Oxidative stress nationallicence Iron toxicity nationallicence Antioxidant enzymes nationallicence Bottcher Alexandra Departamento de Biologia Vegetal, Instituto de Biologia, CP 6109, Universidade Estadual de Campinas, 13083-970, Campinas, SP, Brazil aut Nobile Paula Centro de Recursos Genéticos, Instituto Agronômico de Campinas, 13012-970, Campinas, São Paulo, Brazil aut Martins Paula Departamento de Genética, Escola Superior de Agricultura Luiz de Queiroz, Universidade de São Paulo, 13418-900, Piracicaba, SP, Brazil aut Conte Fábio Departamento de Genética Médica, Faculdade de Ciências Médicas, CP 6111, Universidade Estadual de Campinas, 13083-970, Campinas, SP, Brazil aut Azevedo Ricardo Departamento de Genética, Escola Superior de Agricultura Luiz de Queiroz, Universidade de São Paulo, 13418-900, Piracicaba, SP, Brazil aut Mazzafera Paulo Departamento de Biologia Vegetal, Instituto de Biologia, CP 6109, Universidade Estadual de Campinas, 13083-970, Campinas, SP, Brazil aut BioMetals Springer Netherlands 24/2(2011-04-01), 225-237 0966-0844 24:2<225 2011 24 10534 https://doi.org/10.1007/s10534-010-9388-z text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1007/s10534-010-9388-z text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Bottcher Alexandra Departamento de Biologia Vegetal, Instituto de Biologia, CP 6109, Universidade Estadual de Campinas, 13083-970, Campinas, SP, Brazil aut NATIONALLICENCE 700 1- Nobile Paula Centro de Recursos Genéticos, Instituto Agronômico de Campinas, 13012-970, Campinas, São Paulo, Brazil aut NATIONALLICENCE 700 1- Martins Paula Departamento de Genética, Escola Superior de Agricultura Luiz de Queiroz, Universidade de São Paulo, 13418-900, Piracicaba, SP, Brazil aut NATIONALLICENCE 700 1- Conte Fábio Departamento de Genética Médica, Faculdade de Ciências Médicas, CP 6111, Universidade Estadual de Campinas, 13083-970, Campinas, SP, Brazil aut NATIONALLICENCE 700 1- Azevedo Ricardo Departamento de Genética, Escola Superior de Agricultura Luiz de Queiroz, Universidade de São Paulo, 13418-900, Piracicaba, SP, Brazil aut NATIONALLICENCE 700 1- Mazzafera Paulo Departamento de Biologia Vegetal, Instituto de Biologia, CP 6109, Universidade Estadual de Campinas, 13083-970, Campinas, SP, Brazil aut NATIONALLICENCE 773 0- BioMetals Springer Netherlands 24/2(2011-04-01), 225-237 0966-0844 24:2<225 2011 24 10534 Metadata rights reserved Springer special CC-BY-NC licence nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer