caa a22 4500 445322136 CHVBK 20180317142707.0 cr unu---uuuuu 170323e20110401xx s 000 0 eng 10.1007/s10534-010-9401-6 doi (NATIONALLICENCE)springer-10.1007/s10534-010-9401-6 Characterization and expression of chicken selenoprotein W [Elektronische Daten] [Bor-Rung Ou, Mei-Jung Jiang, Chao-Hsiang Lin, Yu-Chuan Liang, Kuei-Jen Lee, Jan-Ying Yeh] As an essential trace element, selenium (Se) deficiency results in White Muscle Disease in livestock and Keshan disease in humans. The main objectives of this study were to clone and characterize the chicken selenoprotein W (SeW) gene and investigate SeW mRNA expression in chicken tissues. The deduced amino acid (AA) sequence of chicken SeW contains 85 AAs with UAG as the stop codon. Like all SeW genes identified in different species, chicken SeW contains one well-conserved selenocysteine (Sec) at the 13th position encoded by the UGA codon. The proposed glutathione (GSH)-binding site at the Cys37 of SeW is not conserved in the chicken, but Cys9 and Sec13, with possible GSH binding, are conserved in SeWs identified from all species. There are 23-59% and 50-61% homology in cDNA and deduced AA sequences of SeW, respectively, between the chicken and other species. The predicted secondary structure of chicken SeW mRNA indicates that the selenocysteine insertion sequence element is type II with invariant adenosines within the apical bulge. The SeW mRNA expression is high in skeletal muscle followed by brain, but extremely low in other tissues from chickens fed a commercial maize-based diet. The SeW gene is ubiquitously expressed in heart, skeletal muscle, brain, testis, spleen, kidney, lung, liver, stomach and pancreas in chickens fed a commercial diet supplemented with sodium selenite. These results indicate that dietary selenium supplementation regulates SeW gene expression in the chicken and skeletal muscle is the most responsive tissue when dietary Se content is low. Springer Science+Business Media, LLC., 2011 Selenoprotein W nationallicence mRNA expression nationallicence Selenium nationallicence cDNA sequence nationallicence Chicken nationallicence Ou Bor-Rung Department of Animal Science and Biotechnology, Tunghai University, 407, Taichung, Taiwan, ROC aut Jiang Mei-Jung Department of Biotechnology, Asia University, 500 Lioufeng Rd., Wufeng Township, 413, Taichung, Taiwan, ROC aut Lin Chao-Hsiang Department of Biotechnology, Asia University, 500 Lioufeng Rd., Wufeng Township, 413, Taichung, Taiwan, ROC aut Liang Yu-Chuan Department of Biotechnology, Asia University, 500 Lioufeng Rd., Wufeng Township, 413, Taichung, Taiwan, ROC aut Lee Kuei-Jen Department of Bioinformatics, Asia University, Wufeng Township, 413, Taichung, Taiwan, ROC aut Yeh Jan-Ying Department of Biotechnology, Asia University, 500 Lioufeng Rd., Wufeng Township, 413, Taichung, Taiwan, ROC aut BioMetals Springer Netherlands 24/2(2011-04-01), 323-333 0966-0844 24:2<323 2011 24 10534 https://doi.org/10.1007/s10534-010-9401-6 text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1007/s10534-010-9401-6 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Ou Bor-Rung Department of Animal Science and Biotechnology, Tunghai University, 407, Taichung, Taiwan, ROC aut NATIONALLICENCE 700 1- Jiang Mei-Jung Department of Biotechnology, Asia University, 500 Lioufeng Rd., Wufeng Township, 413, Taichung, Taiwan, ROC aut NATIONALLICENCE 700 1- Lin Chao-Hsiang Department of Biotechnology, Asia University, 500 Lioufeng Rd., Wufeng Township, 413, Taichung, Taiwan, ROC aut NATIONALLICENCE 700 1- Liang Yu-Chuan Department of Biotechnology, Asia University, 500 Lioufeng Rd., Wufeng Township, 413, Taichung, Taiwan, ROC aut NATIONALLICENCE 700 1- Lee Kuei-Jen Department of Bioinformatics, Asia University, Wufeng Township, 413, Taichung, Taiwan, ROC aut NATIONALLICENCE 700 1- Yeh Jan-Ying Department of Biotechnology, Asia University, 500 Lioufeng Rd., Wufeng Township, 413, Taichung, Taiwan, ROC aut NATIONALLICENCE 773 0- BioMetals Springer Netherlands 24/2(2011-04-01), 323-333 0966-0844 24:2<323 2011 24 10534 Metadata rights reserved Springer special CC-BY-NC licence nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer