caa a22 4500 445322543 CHVBK 20180317142708.0 cr unu---uuuuu 170323e20111101xx s 000 0 eng 10.1007/s10681-011-0525-8 doi (NATIONALLICENCE)springer-10.1007/s10681-011-0525-8 Genetic analysis and identification of DNA markers linked to a novel Phytophthora sojae resistance gene in the Japanese soybean cultivar Waseshiroge [Elektronische Daten] [T. Sugimoto, S. Yoshida, A. Kaga, M. Hajika, K. Watanabe, M. Aino, K. Tatsuda, R. Yamamoto, T. Matoh, D. Walker, A. Biggs, M. Ishimoto] The Glycine max (L.) Merr. cultivar Waseshiroge is highly resistant to several races of Phytophthora sojae in Japan. In order to determine which Rps gene might be present in Waseshiroge, 15 differential cultivars were challenged with 12 P. sojae isolates. None had a reaction pattern identical to that of Waseshiroge, indicating that Waseshiroge may contain a novel Rps gene. In order to characterize the inheritance of Waseshiroge resistance to P. sojae isolates, 98 F2 progeny and 94 F7:8 lines were produced from crosses between the susceptible cultivar Tanbakuro and Waseshiroge. Chi-square tests indicated that segregation fit a 3:1 ratio for resistance and susceptibility in two F2 sub-populations of 42 and 56 seedlings. This and a 46.27:1.46:46.27 (or 63:2:63) ratio for resistance: segregation: susceptibility among the 94 F7:8 lines indicated that resistance was controlled by a single dominant gene. DNA analyses were carried out on Tanbakuro, Waseshiroge and the 94 F7:8 lines, and a linkage map was constructed with 17 SSR markers and nine new primer pairs that amplify marker loci linked to Rps1 on soybean chromosome 3 (linkage group N). The closest markers, Satt009 and T000304487l, map to locations 0.9 and 1.6cM on each side of the estimated position of the Rps gene, respectively. The results showed that the Rps gene in Waseshiroge is either allelic to Rps1, or resides at a tightly linked locus in a gene cluster. A three-way-contingency table analysis indicated that marker-assisted selection with the two flanking markers could be used in the development of new resistant cultivars. Springer Science+Business Media B.V., 2011 Glycine max nationallicence Marker-assisted selection nationallicence Rps gene nationallicence Soybean nationallicence SSR analysis nationallicence Sugimoto T. Hyogo Agricultural Institute for Agriculture, Forestry and Fisheries, 1533 Minamino-oka, 679-0198, Kasai, Hyogo, Japan aut Yoshida S. Hyogo Agricultural Institute for Agriculture, Forestry and Fisheries, 1533 Minamino-oka, 679-0198, Kasai, Hyogo, Japan aut Kaga A. National Institute of Agrobiological Sciences, Kannondai 2-1-2, 305-8602, Tsukuba, Ibaraki, Japan aut Hajika M. NARO Institute of Crop Science, National Agriculture and Food Research Organization, Kannondai 2-1-18, 305-8518, Tsukuba, Ibaraki, Japan aut Watanabe K. Hyogo Agricultural Institute for Agriculture, Forestry and Fisheries, 1533 Minamino-oka, 679-0198, Kasai, Hyogo, Japan aut Aino M. Hyogo Agricultural Institute for Agriculture, Forestry and Fisheries, 1533 Minamino-oka, 679-0198, Kasai, Hyogo, Japan aut Tatsuda K. Hyogo Agricultural Institute for Agriculture, Forestry and Fisheries, 1533 Minamino-oka, 679-0198, Kasai, Hyogo, Japan aut Yamamoto R. National Agricultural Research Center for Tohoku Region (NARCT), 4 Akahira, Shimo-kuriyagawa, 020-0198, Morioka, Iwate, Japan aut Matoh T. Division of Applied Life Sciences, Laboratory of Plant Nutrition, Graduate School of Agriculture, Kyoto University, Oiwake, Kitashirakawa, 606-8502, Sakyo-ku, Kyoto, Japan aut Walker D. USDA-ARS, Soybean/Maize Germplasm, Pathology, and Genetics Research Unit, 232 National Soybean Research Center, 1101W. Peabody Dr, 61801, Urbana, IL, USA aut Biggs A. Kearneysville Tree Fruit Research and Education Center, West Virginia University, P.O. Box 609, 25430, Kearneysville, WV, USA aut Ishimoto M. National Institute of Agrobiological Sciences, Kannondai 2-1-2, 305-8602, Tsukuba, Ibaraki, Japan aut Euphytica Springer Netherlands 182/1(2011-11-01), 133-145 0014-2336 182:1<133 2011 182 10681 https://doi.org/10.1007/s10681-011-0525-8 text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1007/s10681-011-0525-8 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Sugimoto T. Hyogo Agricultural Institute for Agriculture, Forestry and Fisheries, 1533 Minamino-oka, 679-0198, Kasai, Hyogo, Japan aut NATIONALLICENCE 700 1- Yoshida S. Hyogo Agricultural Institute for Agriculture, Forestry and Fisheries, 1533 Minamino-oka, 679-0198, Kasai, Hyogo, Japan aut NATIONALLICENCE 700 1- Kaga A. National Institute of Agrobiological Sciences, Kannondai 2-1-2, 305-8602, Tsukuba, Ibaraki, Japan aut NATIONALLICENCE 700 1- Hajika M. NARO Institute of Crop Science, National Agriculture and Food Research Organization, Kannondai 2-1-18, 305-8518, Tsukuba, Ibaraki, Japan aut NATIONALLICENCE 700 1- Watanabe K. Hyogo Agricultural Institute for Agriculture, Forestry and Fisheries, 1533 Minamino-oka, 679-0198, Kasai, Hyogo, Japan aut NATIONALLICENCE 700 1- Aino M. Hyogo Agricultural Institute for Agriculture, Forestry and Fisheries, 1533 Minamino-oka, 679-0198, Kasai, Hyogo, Japan aut NATIONALLICENCE 700 1- Tatsuda K. Hyogo Agricultural Institute for Agriculture, Forestry and Fisheries, 1533 Minamino-oka, 679-0198, Kasai, Hyogo, Japan aut NATIONALLICENCE 700 1- Yamamoto R. National Agricultural Research Center for Tohoku Region (NARCT), 4 Akahira, Shimo-kuriyagawa, 020-0198, Morioka, Iwate, Japan aut NATIONALLICENCE 700 1- Matoh T. Division of Applied Life Sciences, Laboratory of Plant Nutrition, Graduate School of Agriculture, Kyoto University, Oiwake, Kitashirakawa, 606-8502, Sakyo-ku, Kyoto, Japan aut NATIONALLICENCE 700 1- Walker D. USDA-ARS, Soybean/Maize Germplasm, Pathology, and Genetics Research Unit, 232 National Soybean Research Center, 1101W. Peabody Dr, 61801, Urbana, IL, USA aut NATIONALLICENCE 700 1- Biggs A. Kearneysville Tree Fruit Research and Education Center, West Virginia University, P.O. Box 609, 25430, Kearneysville, WV, USA aut NATIONALLICENCE 700 1- Ishimoto M. National Institute of Agrobiological Sciences, Kannondai 2-1-2, 305-8602, Tsukuba, Ibaraki, Japan aut NATIONALLICENCE 773 0- Euphytica Springer Netherlands 182/1(2011-11-01), 133-145 0014-2336 182:1<133 2011 182 10681 Metadata rights reserved Springer special CC-BY-NC licence nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer