caa a22 4500 445322683 CHVBK 20180317142709.0 cr unu---uuuuu 170323e20111201xx s 000 0 eng 10.1007/s10681-011-0484-0 doi (NATIONALLICENCE)springer-10.1007/s10681-011-0484-0 Identification of sugarcane microsatellites associated to sugar content in sugarcane and transferability to other cereal genomes [Elektronische Daten] [R. Singh, R. Singh, S. Singh, M. Sharma] Microsatellites or simple sequence repeats (SSRs) markers are very informative for various applications in genetics and breeding. Information obtained with these markers has contributed to a better understanding of evolution and the complexity of the sugarcane genome. With the objective of identifying a large set of polymorphic microsatellite markers designated as Unigene derived Sugarcane Microsatellite (UGSM) and Sugarcane Enriched Genomic Microsatellite (SEGMS), 351 UGSM and 36 SEGMS were tested to find out informative SSRs marker for sugar content. These markers were screened and validated for their use in genetic diversity, cross transferability and comparative linkage potential in high and low sugar bulk of two segregating progenies and twenty each, cultivated high and low sugar cultivars. 158 (40.83%) of the microsatellite markers (144-UGSM: 14-SEGMS) were found to be highly robust and polymorphic. Cross amplification was estimated among nineteen accessions of six sugarcane cultivars, one inter specific hybrids, five related species, four related genera, and three divergent genera by using 27 UGSM primers. Analysis of 388 alleles, amplified by these markers, indicated the high number of observed allele ranged from 2 to 26, with an average of 14.37 alleles detected per locus. High level of polymorphism detected by these markers among sugarcane species, genera and cultivars was 96.3%, while cross-transferability rate was 98.0% within Saccharum complex and 88.27% to cereals. Wide range of genetic diversity (0.33-0.79 with an average of 0.56) assayed with UGSM markers suggested their importance in various genotypic applications in sugarcane. Springer Science+Business Media B.V., 2011 Microsatellite markers nationallicence UGSM nationallicence SEGM nationallicence Cross transferability nationallicence Saccharum complex nationallicence Sugarcane nationallicence Singh R. Centre for Sugarcane Biotechnology, Sugarcane Research Institute, UP Council of Sugarcane Research, Shahjahanpur, UP, India aut Singh S. Centre for Sugarcane Biotechnology, Sugarcane Research Institute, UP Council of Sugarcane Research, Shahjahanpur, UP, India aut Sharma M. Centre for Sugarcane Biotechnology, Sugarcane Research Institute, UP Council of Sugarcane Research, Shahjahanpur, UP, India aut Euphytica Springer Netherlands 182/3(2011-12-01), 335-354 0014-2336 182:3<335 2011 182 10681 https://doi.org/10.1007/s10681-011-0484-0 text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1007/s10681-011-0484-0 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Singh R. Centre for Sugarcane Biotechnology, Sugarcane Research Institute, UP Council of Sugarcane Research, Shahjahanpur, UP, India aut NATIONALLICENCE 700 1- Singh S. Centre for Sugarcane Biotechnology, Sugarcane Research Institute, UP Council of Sugarcane Research, Shahjahanpur, UP, India aut NATIONALLICENCE 700 1- Sharma M. Centre for Sugarcane Biotechnology, Sugarcane Research Institute, UP Council of Sugarcane Research, Shahjahanpur, UP, India aut NATIONALLICENCE 773 0- Euphytica Springer Netherlands 182/3(2011-12-01), 335-354 0014-2336 182:3<335 2011 182 10681 Metadata rights reserved Springer special CC-BY-NC licence nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer