caa a22 4500 445322705 CHVBK 20180317142709.0 cr unu---uuuuu 170323e20111201xx s 000 0 eng 10.1007/s10681-011-0486-y doi (NATIONALLICENCE)springer-10.1007/s10681-011-0486-y Meiotic behavior in Brachiaria humidicola ( Poaceae ) hybrids [Elektronische Daten] [Gléia Ricci, Alice de Souza-Kaneshima, Maria Pagliarini, Cacilda Valle] In the genus Brachiaria, genetic variation can be exploited directly from germplasm collections or released using sexual reproduction in normally apomictic polyploids. The discovery of a natural sexual polyploid accession H031 of Brachiaria humidicola collected in Africa, opened new opportunities to exploit the genetic variation in this species. This accession was crossed with an apomictic cultivar BRS Tupi with the same chromosome number (2n=36) and 361 F1 hybrids were obtained. Following visual selection for leafiness, vigor, growth habit, and the mode of reproduction, 50 hybrids were selected for further agronomic evaluation. The parents and 45 of the 50 selected hybrids were evaluated for the regularity of meiosis. In the female parent (H031), meiosis was somewhat irregular, with 16.3% of abnormal tetrads, whereas the male (cv. BRS Tupi) meiosis was very regular, with only 3.1% of abnormal tetrads. Among hybrids (sexual and apomictic), the percentage of abnormal tetrads ranged from 15.8 to 98.3%. The abnormalities included irregular chromosome segregation, chromosome stickiness and the absence of cytokinesis. Considering that apomixis in the genus Brachiaria is pseudogamic, and that meiotic aberrations can compromise pollen viability, the results of this study present another parameter to aid selection for more stable microsporogenesis. Apomictic derivatives with stable meiosis are candidates for new cultivars whereas sexual hybrids can be retained in breeding for another round of recombination. Springer Science+Business Media B.V., 2011 Brachiaria humidicola nationallicence Breeding nationallicence Forage nationallicence Hybrids nationallicence Microsporogenesis nationallicence Mode of reproduction nationallicence Ricci Gléia Department of Cell Biology and Genetics, State University of Maringá, 87020-900, Maringá, PR, Brazil aut de Souza-Kaneshima Alice Department of Cell Biology and Genetics, State University of Maringá, 87020-900, Maringá, PR, Brazil aut Pagliarini Maria Department of Cell Biology and Genetics, State University of Maringá, 87020-900, Maringá, PR, Brazil aut Valle Cacilda Embrapa Beef Cattle, 154, 79002-970, Campo Grande, MS, Brazil aut Euphytica Springer Netherlands 182/3(2011-12-01), 355-361 0014-2336 182:3<355 2011 182 10681 https://doi.org/10.1007/s10681-011-0486-y text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1007/s10681-011-0486-y text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Ricci Gléia Department of Cell Biology and Genetics, State University of Maringá, 87020-900, Maringá, PR, Brazil aut NATIONALLICENCE 700 1- de Souza-Kaneshima Alice Department of Cell Biology and Genetics, State University of Maringá, 87020-900, Maringá, PR, Brazil aut NATIONALLICENCE 700 1- Pagliarini Maria Department of Cell Biology and Genetics, State University of Maringá, 87020-900, Maringá, PR, Brazil aut NATIONALLICENCE 700 1- Valle Cacilda Embrapa Beef Cattle, 154, 79002-970, Campo Grande, MS, Brazil aut NATIONALLICENCE 773 0- Euphytica Springer Netherlands 182/3(2011-12-01), 355-361 0014-2336 182:3<355 2011 182 10681 Metadata rights reserved Springer special CC-BY-NC licence nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer