caa a22 4500 445322810 CHVBK 20180317142709.0 cr unu---uuuuu 170323e20111101xx s 000 0 eng 10.1007/s10681-011-0490-2 doi (NATIONALLICENCE)springer-10.1007/s10681-011-0490-2 Evaluation of androgenic competence through anther culture in common eggplant and related species [Elektronische Daten] [Pablo Salas, Jaime Prohens, José Seguí-Simarro] Anther culture is a convenient technique to obtain androgenic haploid and doubled haploid (DH) plants. In common eggplant (Solanum melongena), this technique has been used to develop DH pure lines for producing uniform F1 hybrid seed of some commercial varieties. However, a comprehensive study of the variation of this useful trait among different materials of common eggplant and related species is still lacking. In this work, we studied the androgenic response of 12 accessions of common eggplant and related materials from the primary (eggplant complex) and secondary genepools. We cultured anthers of all the accessions under the same experimental conditions, and studied their competence to produce calli, embryos and plants, as well as the quality and origin of the embryos produced. In our conditions, anthers of 11 out of the 12 accessions produced somatic calli, whereas only 5 also produced microspore-derived embryos, with variable results in terms of embryo quality and of frequency of embryo induction and plant germination. Embryos of responding accessions were initially haploid, and reached the DH status, verified with SSR markers, after a defined period of culture. In addition to other aspects common to many androgenesis-responsive species, our results allowed us to extract conclusions particular to common eggplant and relatives, including the difficulty for finding sources of androgenic competence out of S. melongena, the reduced impact of calli in the production of non-DH individuals, and the need to avoid the occurrence of severe anatomical and functional problems in the apex of most embryos, which seriously reduces their germinative success. Springer Science+Business Media B.V., 2011 Androgenesis nationallicence Anther culture nationallicence Doubled haploid nationallicence Microspore embryogenesis nationallicence Solanum aethiopicum nationallicence Solanum incanum nationallicence Solanum macrocarpon nationallicence Solanum melongena nationallicence Salas Pablo COMAV—Universitat Politècnica de València, CPI, Edificio 8E—Escalera I, Camino de Vera, s/n, 46022, Valencia, Spain aut Prohens Jaime COMAV—Universitat Politècnica de València, CPI, Edificio 8E—Escalera I, Camino de Vera, s/n, 46022, Valencia, Spain aut Seguí-Simarro José COMAV—Universitat Politècnica de València, CPI, Edificio 8E—Escalera I, Camino de Vera, s/n, 46022, Valencia, Spain aut Euphytica Springer Netherlands 182/2(2011-11-01), 261-274 0014-2336 182:2<261 2011 182 10681 https://doi.org/10.1007/s10681-011-0490-2 text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1007/s10681-011-0490-2 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Salas Pablo COMAV—Universitat Politècnica de València, CPI, Edificio 8E—Escalera I, Camino de Vera, s/n, 46022, Valencia, Spain aut NATIONALLICENCE 700 1- Prohens Jaime COMAV—Universitat Politècnica de València, CPI, Edificio 8E—Escalera I, Camino de Vera, s/n, 46022, Valencia, Spain aut NATIONALLICENCE 700 1- Seguí-Simarro José COMAV—Universitat Politècnica de València, CPI, Edificio 8E—Escalera I, Camino de Vera, s/n, 46022, Valencia, Spain aut NATIONALLICENCE 773 0- Euphytica Springer Netherlands 182/2(2011-11-01), 261-274 0014-2336 182:2<261 2011 182 10681 Metadata rights reserved Springer special CC-BY-NC licence nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer