caa a22 4500 445322837 CHVBK 20180317142709.0 cr unu---uuuuu 170323e20111101xx s 000 0 eng 10.1007/s10681-011-0407-0 doi (NATIONALLICENCE)springer-10.1007/s10681-011-0407-0 Mapping a dominant negative mutation for triforine sensitivity in lettuce and its use as a selectable marker for detecting hybrids [Elektronische Daten] [Ivan Simko, Ryan Hayes, María Truco, Richard Michelmore] Some lettuce cultivars are highly sensitive to triforine, an inhibitor of sterol biosynthesis found in some commercial systemic fungicides. First symptoms of a sensitive reaction are usually observed within 24-48h after treatment and include severe wilting, necrosis and rapid plant death. We mapped a single dominant gene (Tr) that confers sensitivity of lettuce to triforine to linkage group 1 of the integrated genetic map of lettuce. The occurrence of sensitivity is not uniform across horticultural types of lettuce. While over 80% of green-romaine lettuce cultivars tested were sensitive, most cultivars of all other lettuce types were insensitive to triforine. All accessions of wild Lactuca spp. were insensitive to triforine. Allelism tests using F1 and F2 progeny revealed that sensitive cultivars of all horticultural types likely carry the same Tr gene. The dominant allele for sensitivity found in cultivated lettuce probably had a monophyletic origin. The reaction to triforine can be used as a marker for detecting hybrids originating from a cross between phenotypically similar parents with different responses to triforine treatment. It also provides an indication of genotypes for which applications of triforine-containing fungicides are inappropriate. Springer Science+Business Media B.V. (outside the USA), 2011 Dominant mutation nationallicence Lettuce nationallicence Selectable marker nationallicence Sterol biosynthesis inhibitor nationallicence Triforine nationallicence cM : CentiMorgans nationallicence RILs : Recombinant-inbred lines nationallicence Tr gene : A dominant gene that confers sensitivity to triforine in lettuce nationallicence Simko Ivan Crop Improvement and Protection Research Unit, USDA-ARS, 1636 East Alisal Street, 93905, Salinas, CA, USA aut Hayes Ryan Crop Improvement and Protection Research Unit, USDA-ARS, 1636 East Alisal Street, 93905, Salinas, CA, USA aut Truco María The Genome Center and Department of Plant Sciences, University of California, 451 Health Sciences Drive, 95616, Davis, CA, USA aut Michelmore Richard The Genome Center and Department of Plant Sciences, University of California, 451 Health Sciences Drive, 95616, Davis, CA, USA aut Euphytica Springer Netherlands 182/2(2011-11-01), 157-166 0014-2336 182:2<157 2011 182 10681 https://doi.org/10.1007/s10681-011-0407-0 text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1007/s10681-011-0407-0 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Simko Ivan Crop Improvement and Protection Research Unit, USDA-ARS, 1636 East Alisal Street, 93905, Salinas, CA, USA aut NATIONALLICENCE 700 1- Hayes Ryan Crop Improvement and Protection Research Unit, USDA-ARS, 1636 East Alisal Street, 93905, Salinas, CA, USA aut NATIONALLICENCE 700 1- Truco María The Genome Center and Department of Plant Sciences, University of California, 451 Health Sciences Drive, 95616, Davis, CA, USA aut NATIONALLICENCE 700 1- Michelmore Richard The Genome Center and Department of Plant Sciences, University of California, 451 Health Sciences Drive, 95616, Davis, CA, USA aut NATIONALLICENCE 773 0- Euphytica Springer Netherlands 182/2(2011-11-01), 157-166 0014-2336 182:2<157 2011 182 10681 Metadata rights reserved Springer special CC-BY-NC licence nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer