caa a22 4500 445322861 CHVBK 20180317142710.0 cr unu---uuuuu 170323e20111101xx s 000 0 eng 10.1007/s10681-011-0444-8 doi (NATIONALLICENCE)springer-10.1007/s10681-011-0444-8 Reciprocal differences in DNA sequence and methylation status of the pollen DNA between F1 hybrids of Solanum tuberosum × S. demissum [Elektronische Daten] [Rena Sanetomo, Kazuyoshi Hosaka] Reciprocal differences, mostly caused by cytoplasmic effects, are frequently observed in interspecific hybrids. Previously, we found that crosses onto Solanum demissum were much successful with the pollen of interspecific hybrids between S. tuberosum as female and S. demissum as male (TD hybrids) than the reciprocal ones (DT hybrids). To elucidate this reciprocally different crossability, we analyzed the pollen DNA of TD and DT using methylation-sensitive amplified polymorphism (MSAP) analysis. Using 126 primer combinations, MSAP analysis revealed 57 different bands between bulked pollen DNA samples of TD and DT. Individual examination of 16 TD and 9 DT plants disclosed eight bands uniformly different between TD and DT. Their sequencing results revealed two pairs of bands to be identical to each other, resulting in six distinct sequences. As expected, one band shared high homology with chloroplast DNA, and another one with mitochondrial DNA. However, one band that was apparently different at DNA sequence level and maternally transmitted from S. demissum, showed no homology with any known sequence. The remaining three bands were of DNA methylation level differences with no or uncertain homology to known sequences. To our knowledge, this is the first report detecting reciprocal differences in DNA sequence or DNA methylation other than those in cytoplasmic DNA. Springer Science+Business Media B.V., 2011 Solanum tuberosum nationallicence Reciprocal difference nationallicence Solanum demissum nationallicence DNA methylation nationallicence Pollen DNA nationallicence MSAP analysis nationallicence Sanetomo Rena Graduate School of Agricultural Science, Kobe University, Nada, 657-8501, Kobe, Hyogo, Japan aut Hosaka Kazuyoshi National Agricultural Research Center for Hokkaido Region, Shinsei, Memuro, 082-0081, Hokkaido, Japan aut Euphytica Springer Netherlands 182/2(2011-11-01), 219-229 0014-2336 182:2<219 2011 182 10681 https://doi.org/10.1007/s10681-011-0444-8 text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1007/s10681-011-0444-8 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Sanetomo Rena Graduate School of Agricultural Science, Kobe University, Nada, 657-8501, Kobe, Hyogo, Japan aut NATIONALLICENCE 700 1- Hosaka Kazuyoshi National Agricultural Research Center for Hokkaido Region, Shinsei, Memuro, 082-0081, Hokkaido, Japan aut NATIONALLICENCE 773 0- Euphytica Springer Netherlands 182/2(2011-11-01), 219-229 0014-2336 182:2<219 2011 182 10681 Metadata rights reserved Springer special CC-BY-NC licence nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer