caa a22 4500 445323140 CHVBK 20180317142711.0 cr unu---uuuuu 170323e20110601xx s 000 0 eng 10.1007/s10681-010-0339-0 doi (NATIONALLICENCE)springer-10.1007/s10681-010-0339-0 Determination of responses of different bean cultivars against races of Pseudomonas syringae pv phaseolicola , causal agent of halo blight of bean [Elektronische Daten] [İmam Bozkurt, Soner Soylu] Use of resistant plant varieties combined with other disease management practices is regarded as the most practical approach to control of seed-borne bacterial disease agents. In this study, responses of different bean cultivars to nine different races of Pseudomonas syringae pv phaseolicola, the causal agent of bacterial halo blight of common bean (Phaseolus vulgaris L.), were determined. During compatible interaction in susceptible cultivars, virulent bacterial races caused water soaked lesion at sites of inoculation. Similar lesions developed in moderately resistant cultivars but symptoms were later associated with more tissue browning around the sites of inoculation. In contrast, the resistant response, produced the characteristic hypersensitive reaction (HR), was characterized as a small discrete browning and tissue collapse at site of inoculation. No local cultivars showed complete resistance to all races tested. Bean cultivars Sehirali-90 and Göynük-98 were found to be resistant or moderately resistant to five different bacterial races. Bean cultivar, Karacaşehir-90, on the other hand, was found to be resistant or moderately resistant to six different bacterial races. Analysis of bacterial growth and the accumulation of isoflavonoid bean phytoalexin, phaseollin in planta were carried out for tissues expressing compatible and incompatible interactions to enable a link to be made between reaction phenotypes and restriction of bacterial growth and phytoalexin accumulations. Development of the HR was clearly associated with the restricted multiplication of bacteria during incompatible interactions. A time-course accumulation analysis on pods treated with different races of bacterial agent showed that a strong correlation was observed between the timing and extent of cell death and accumulation of phaseollin, being rapid and extensive in incompatible interactions compared to compatible interaction. Springer Science+Business Media B.V., 2011 Bean nationallicence Halo blight nationallicence Phaseollin nationallicence Phytoalexin nationallicence Resistance nationallicence Bozkurt İmam Department of Plant Protection, Mustafa Kemal University, Faculty of Agriculture, 31034, Antakya, Hatay, Turkey aut Soylu Soner Department of Plant Protection, Mustafa Kemal University, Faculty of Agriculture, 31034, Antakya, Hatay, Turkey aut Euphytica Springer Netherlands 179/3(2011-06-01), 417-425 0014-2336 179:3<417 2011 179 10681 https://doi.org/10.1007/s10681-010-0339-0 text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1007/s10681-010-0339-0 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Bozkurt İmam Department of Plant Protection, Mustafa Kemal University, Faculty of Agriculture, 31034, Antakya, Hatay, Turkey aut NATIONALLICENCE 700 1- Soylu Soner Department of Plant Protection, Mustafa Kemal University, Faculty of Agriculture, 31034, Antakya, Hatay, Turkey aut NATIONALLICENCE 773 0- Euphytica Springer Netherlands 179/3(2011-06-01), 417-425 0014-2336 179:3<417 2011 179 10681 Metadata rights reserved Springer special CC-BY-NC licence nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer