caa a22 4500 445323167 CHVBK 20180317142711.0 cr unu---uuuuu 170323e20110601xx s 000 0 eng 10.1007/s10681-011-0351-z doi (NATIONALLICENCE)springer-10.1007/s10681-011-0351-z Screening of wild crucifers for resistance to mustard aphid, Lipaphis erysimi (Kaltenbach) and attempt at introgression of resistance gene(s) from Brassica fruticulosa to Brassica juncea [Elektronische Daten] [Sarwan Kumar, Chhaya Atri, Manjeet Sangha, S. Banga] A diverse array of wild and weedy crucifers was screened under laboratory conditions for their resistance to Lipaphis erysimi (Kaltenbach) (Homoptera: Aphididae). Among these, Brassica fruticulosa and Brassica montana were found to be the most promising. The availability of a synthetic amphiploid, AD-4(B. fruticulosa×Brassica rapa var. brown sarson) as well as a set of Brassica juncea lines carrying genomic introgressions from B. fruticulosa allowed us to investigate B. fruticulosa resistance in greater detail. This assessment was carried out along with susceptible check B. rapa ssp. brown sarson cv. BSH-1 in a series of choice and no choice experiments. The mustard aphid showed maximum preference for feeding on BSH-1 while least preference was recorded for B. fruticulosa followed by AD-4 as evidenced by the number of aphids that settled on circular leaf bits of these genotypes 24 and 48h after release in a choice experiment. Brassica fruticulosa exhibited strong antibiosis against mustard aphid in no choice experiment and all the released aphids died within 5-8days of their release, while the maximum survival (76.7%) was recorded on BSH-1. The survival on AD-4 (40%) was significantly lower than that on BSH-1. Almost similar trend was observed with respect to other demographic parameters of L. erysimi viz. development time, fecundity and longevity. In the screen house studies, there was no seedling mortality in B. fruticulosa and AD-4 after 30days of aphid release while 80% mortality was observed on BSH-1. Excellent variation for aphid resistance was recorded in B. juncea introgression lines, emphasizing heritable nature of fruticulosa resistance. The biochemical analysis suggested the possibility of high concentration of lectins to be associated with low aphid infestation in B. fruticulosa. Springer Science+Business Media B.V., 2011 Antibiosis nationallicence Brassica montana nationallicence Cabbage Aphid nationallicence Host Plant Resistance nationallicence Introgression lines nationallicence Wild crucifers nationallicence Kumar Sarwan Department of Plant Breeding and Genetics, Punjab Agricultural University, 141004, Ludhiana, Punjab, India aut Atri Chhaya Department of Plant Breeding and Genetics, Punjab Agricultural University, 141004, Ludhiana, Punjab, India aut Sangha Manjeet Department of Plant Breeding and Genetics, Punjab Agricultural University, 141004, Ludhiana, Punjab, India aut Banga S. Department of Plant Breeding and Genetics, Punjab Agricultural University, 141004, Ludhiana, Punjab, India aut Euphytica Springer Netherlands 179/3(2011-06-01), 461-470 0014-2336 179:3<461 2011 179 10681 https://doi.org/10.1007/s10681-011-0351-z text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1007/s10681-011-0351-z text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Kumar Sarwan Department of Plant Breeding and Genetics, Punjab Agricultural University, 141004, Ludhiana, Punjab, India aut NATIONALLICENCE 700 1- Atri Chhaya Department of Plant Breeding and Genetics, Punjab Agricultural University, 141004, Ludhiana, Punjab, India aut NATIONALLICENCE 700 1- Sangha Manjeet Department of Plant Breeding and Genetics, Punjab Agricultural University, 141004, Ludhiana, Punjab, India aut NATIONALLICENCE 700 1- Banga S. Department of Plant Breeding and Genetics, Punjab Agricultural University, 141004, Ludhiana, Punjab, India aut NATIONALLICENCE 773 0- Euphytica Springer Netherlands 179/3(2011-06-01), 461-470 0014-2336 179:3<461 2011 179 10681 Metadata rights reserved Springer special CC-BY-NC licence nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer