caa a22 4500 445323175 CHVBK 20180317142711.0 cr unu---uuuuu 170323e20110601xx s 000 0 eng 10.1007/s10681-010-0338-1 doi (NATIONALLICENCE)springer-10.1007/s10681-010-0338-1 An intraspecific genetic map of water yam ( Dioscorea alata L.) based on AFLP markers and QTL analysis for anthracnose resistance [Elektronische Daten] [D. Petro, T. Onyeka, S. Etienne, S. Rubens] Water yam (Dioscorea alata L.) is the most widely cultivated food yams. Despite its importance, its production is limited by anthracnose disease caused by Colletotrichum gloeosporioides (Penz.). The use of resistant yam varieties is the most reliable approach of management of this disease. The speed and precision of breeding can be improved by the development of genetic linkage maps which would provide the basis for locating and hence manipulating quantitative traits such as anthracnose resistance in breeding programmes. An F1 diploid population was developed by crossing ‘Boutou' a female clone (with field resistance to anthracnose) with ‘Pyramide' (susceptible). A linkage map was generated with 523 polymorphic markers from 26 AFLP primer combinations. The resulting map covered a total length of 1538cM and included 20 linkage groups. It is the most saturated of all genetic linkage maps of yam to date. QTL analysis of anthracnose resistance was performed based on response to two isolates of C. gloeosporioides. Resistance to anthracnose appeared to be inherited quantitatively. Using a LOD significance threshold of 2.6 we identified a total of nine QTLs for anthracnose resistance. The phenotypic variance explained by each QTL ranged from 7.0 to 32.9% whereas the total amount of phenotypic variation for anthracnose resistance explained by all significant QTLs varied from 26.4 to 73.7% depending on the isolate and the variable considered. These QTLs displayed isolate-specific resistance as well as broad spectrum resistance. The availability of molecular markers linked to the QTLs of anthracnose resistance will facilitate marker-assisted selection in breeding programmes. Springer Science+Business Media B.V., 2011 Yam nationallicence Dioscorea alata nationallicence Anthracnose nationallicence Genetic mapping nationallicence QTLs (quantitative trait loci) nationallicence Petro D. INRA, UR979 Unité de Recherche en Productions Végétales, 97170, Petit-Bourg, Guadeloupe, France aut Onyeka T. INRA, UR979 Unité de Recherche en Productions Végétales, 97170, Petit-Bourg, Guadeloupe, France aut Etienne S. INRA, UR979 Unité de Recherche en Productions Végétales, 97170, Petit-Bourg, Guadeloupe, France aut Rubens S. INRA, UR979 Unité de Recherche en Productions Végétales, 97170, Petit-Bourg, Guadeloupe, France aut Euphytica Springer Netherlands 179/3(2011-06-01), 405-416 0014-2336 179:3<405 2011 179 10681 https://doi.org/10.1007/s10681-010-0338-1 text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1007/s10681-010-0338-1 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Petro D. INRA, UR979 Unité de Recherche en Productions Végétales, 97170, Petit-Bourg, Guadeloupe, France aut NATIONALLICENCE 700 1- Onyeka T. INRA, UR979 Unité de Recherche en Productions Végétales, 97170, Petit-Bourg, Guadeloupe, France aut NATIONALLICENCE 700 1- Etienne S. INRA, UR979 Unité de Recherche en Productions Végétales, 97170, Petit-Bourg, Guadeloupe, France aut NATIONALLICENCE 700 1- Rubens S. INRA, UR979 Unité de Recherche en Productions Végétales, 97170, Petit-Bourg, Guadeloupe, France aut NATIONALLICENCE 773 0- Euphytica Springer Netherlands 179/3(2011-06-01), 405-416 0014-2336 179:3<405 2011 179 10681 Metadata rights reserved Springer special CC-BY-NC licence nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer