caa a22 4500 445323183 CHVBK 20180317142711.0 cr unu---uuuuu 170323e20110601xx s 000 0 eng 10.1007/s10681-010-0333-6 doi (NATIONALLICENCE)springer-10.1007/s10681-010-0333-6 Characterizing the molecular and morphophysiological diversity of Italian red clover [Elektronische Daten] [Mario Pagnotta, Paolo Annicchiarico, Anna Farina, Sandro Proietti] Red clover (Trifolium pratense L.) is the third major forage species in Europe, but there is limited information on the biodiversity and the genetic structure of landraces and natural populations which evolved in this region. The objective of this study was producing such information for Italian germplasm on the ground of molecular and morphophysiological diversity. The study included 16 Italian natural populations from a wide range of environments, four landraces representing the four traditional commercial ecotypes, and two varieties. Eight morphophysiological traits were assessed in a replicated trial under field conditions, whereas two AFLPs primer combinations with 140 polymorphic markers were recorded on a random sample of 13 genotypes per population. Ordination and classification results based on morphophysiological data clearly kept track of the type of germplasm (i.e. landrace or natural population) and its geographic origin, unlike results based on molecular markers. Euclidean distances among populations based on morphophysiological traits were not correlated with Nei's genetic distances based on molecular markers according to Mantel's test. Geographical distances among landrace or natural population material was correlated with distances based on morphophysiological traits but not with those based on molecular markers. The average within-population variation estimated via molecular markers was about 2.6-fold higher than that among populations, preventing an acceptable discrimination among most populations. On average, natural populations tended to have within-population variation similar to varieties and somewhat lower than landraces. Our results have implications for collection, conservation, exploitation and registration in a sui generis system of red clover genetic resources. Springer Science+Business Media B.V., 2010 Genetic distance nationallicence Genetic resources nationallicence Genetic structure nationallicence Gene flow nationallicence Pagnotta Mario Department of Agrobiology and Agrochemistry, University of Tuscia, Via San Camillo de Lellis, 01100, Viterbo, Italy aut Annicchiarico Paolo CRA-Centro di Ricerca per le Produzioni Foraggere e Lattiero-Casearie, Viale Piacenza 29, 26900, Lodi, Italy aut Farina Anna Department of Agrobiology and Agrochemistry, University of Tuscia, Via San Camillo de Lellis, 01100, Viterbo, Italy aut Proietti Sandro CRA-Centro di Ricerca per le Produzioni Foraggere e Lattiero-Casearie, Viale Piacenza 29, 26900, Lodi, Italy aut Euphytica Springer Netherlands 179/3(2011-06-01), 393-404 0014-2336 179:3<393 2011 179 10681 https://doi.org/10.1007/s10681-010-0333-6 text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1007/s10681-010-0333-6 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Pagnotta Mario Department of Agrobiology and Agrochemistry, University of Tuscia, Via San Camillo de Lellis, 01100, Viterbo, Italy aut NATIONALLICENCE 700 1- Annicchiarico Paolo CRA-Centro di Ricerca per le Produzioni Foraggere e Lattiero-Casearie, Viale Piacenza 29, 26900, Lodi, Italy aut NATIONALLICENCE 700 1- Farina Anna Department of Agrobiology and Agrochemistry, University of Tuscia, Via San Camillo de Lellis, 01100, Viterbo, Italy aut NATIONALLICENCE 700 1- Proietti Sandro CRA-Centro di Ricerca per le Produzioni Foraggere e Lattiero-Casearie, Viale Piacenza 29, 26900, Lodi, Italy aut NATIONALLICENCE 773 0- Euphytica Springer Netherlands 179/3(2011-06-01), 393-404 0014-2336 179:3<393 2011 179 10681 Metadata rights reserved Springer special CC-BY-NC licence nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer