caa a22 4500 445323191 CHVBK 20180317142711.0 cr unu---uuuuu 170323e20110601xx s 000 0 eng 10.1007/s10681-010-0342-5 doi (NATIONALLICENCE)springer-10.1007/s10681-010-0342-5 Development of simple PCR-based markers linked to the Ms locus, a restorer-of-fertility gene in onion ( Allium cepa L.) [Elektronische Daten] [Haejeen Bang, Dong Cho, Kil-Sun Yoo, Moo-Kyoung Yoon, Bhimanagouda Patil, Sunggil Kim] In order to implement reliable marker-assisted selection systems for the restorer-of-fertility locus (Ms) in onions (Allium cepa L.), simple PCR-based codominant markers linked to the Ms locus were developed. Based on the EST probe sequences of previously reported RFLP markers, full-length genomic sequences of the gene encoding putative oligopeptide transporter (OPT) was obtained by RACE. The first intron contained two 108 and 439-bp indel polymorphisms between the two Ms allele-linked OPT alleles. A simple PCR marker for OPT was developed by designing a primer pair on the flanking regions of the 108-bp indel which is created by two tandem repeats. The second simple PCR marker was developed from the EST probe encoding photosystem I subunit O (PsaO). Two 14 and 39-bp tandem repeats were identified from the 5′ upstream sequences of the PsaO-coding gene, which were isolated by genome walking. Three different compositions of these tandem repeats were identified from diverse onion germplasm. A primer set binding to the flanking sequence of these polymorphic repeats was used to amplify three different marker haplotypes. The OPT marker was tightly linked to the Ms locus at a distance of 1.5cM, but the analysis of the linkage relationship showed little linkage disequilibrium between the marker and the Ms locus. Even so, these simple PCR markers are valuable tools for the marker-assisted selection of segregating individuals in onion F1 hybrid breeding programs. Springer Science+Business Media B.V., 2011 Onion ( Allium cepa L.) nationallicence Cytoplasmic male-sterility nationallicence Restorer-of-fertility genes nationallicence Molecular markers nationallicence Marker-assisted selection nationallicence Bang Haejeen Department of Horticultural Sciences, Vegetable and Fruit Improvement Center, Texas A&M University, 77845, College Station, TX, USA aut Cho Dong ONBREETECH Corp, 536-803, Haenam, Korea aut Yoo Kil-Sun Department of Horticultural Sciences, Vegetable and Fruit Improvement Center, Texas A&M University, 77845, College Station, TX, USA aut Yoon Moo-Kyoung National Institute of Horticultural and Herbal Science, RDA, 441-440, Suwon, Korea aut Patil Bhimanagouda Department of Horticultural Sciences, Vegetable and Fruit Improvement Center, Texas A&M University, 77845, College Station, TX, USA aut Kim Sunggil Department of Plant Biotechnology, Biotechnology Research Institute, Chonnam National University, 500-757, Gwangju, Korea aut Euphytica Springer Netherlands 179/3(2011-06-01), 439-449 0014-2336 179:3<439 2011 179 10681 https://doi.org/10.1007/s10681-010-0342-5 text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1007/s10681-010-0342-5 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Bang Haejeen Department of Horticultural Sciences, Vegetable and Fruit Improvement Center, Texas A&M University, 77845, College Station, TX, USA aut NATIONALLICENCE 700 1- Cho Dong ONBREETECH Corp, 536-803, Haenam, Korea aut NATIONALLICENCE 700 1- Yoo Kil-Sun Department of Horticultural Sciences, Vegetable and Fruit Improvement Center, Texas A&M University, 77845, College Station, TX, USA aut NATIONALLICENCE 700 1- Yoon Moo-Kyoung National Institute of Horticultural and Herbal Science, RDA, 441-440, Suwon, Korea aut NATIONALLICENCE 700 1- Patil Bhimanagouda Department of Horticultural Sciences, Vegetable and Fruit Improvement Center, Texas A&M University, 77845, College Station, TX, USA aut NATIONALLICENCE 700 1- Kim Sunggil Department of Plant Biotechnology, Biotechnology Research Institute, Chonnam National University, 500-757, Gwangju, Korea aut NATIONALLICENCE 773 0- Euphytica Springer Netherlands 179/3(2011-06-01), 439-449 0014-2336 179:3<439 2011 179 10681 Metadata rights reserved Springer special CC-BY-NC licence nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer