caa a22 4500 445323221 CHVBK 20180317142711.0 cr unu---uuuuu 170323e20110501xx s 000 0 eng 10.1007/s10681-010-0308-7 doi (NATIONALLICENCE)springer-10.1007/s10681-010-0308-7 Agrobacterium mediated transformation of indica rice ( Oryza sativa L.) for insect resistance [Elektronische Daten] [S. Ignacimuthu, S. Raveendar] The rice leaffolder (RLF), Cnaphalocrocis medinalis is an important pest of rice that causes severe damage in many areas of the world. The plants were transformed with fully modified (plant codon optimized) synthetic Cry1C coding sequences as well as with the hpt and gus genes, coding for hygromycin phosphotransferase and β-glucuronidase, respectively. Cry1C sequences placed under the control of doubled 35S promoter plus the AMV leader sequence, and hpt and gus genes driven by cauliflower mosaic virus 35S promoter, were used in this study. Embryogenic calli after cocultivation with Agrobacterium were selected on the medium containing hygromycin B. A total of 67 hygromycin-resistant plants were regenerated. PCR and Southern blot analyses of primary transformants revealed the stable integration of Cry1C coding sequences into the rice genome with predominant single copy integration. R1 progeny plants disclosed a monogenic pattern (3:1) of transgene segregation as confirmed by molecular analyses. These transgenic lines were highly resistant to rice leaffolder (RLF), Cnaphalocrocis medinalis as revealed by insect bioassay. Springer Science+Business Media B.V., 2010 Rice ( Oryza sativa ) nationallicence Agrobacterium transformation nationallicence Cry1C nationallicence hpt nationallicence Gene transfer nationallicence Transgenic plants nationallicence Insect resistance nationallicence Ignacimuthu S. Plant Biotechnology Unit, Entomology Research Institute, Loyola College, 600 034, Chennai, Tamil Nadu, India aut Raveendar S. Plant Biotechnology Unit, Entomology Research Institute, Loyola College, 600 034, Chennai, Tamil Nadu, India aut Euphytica Springer Netherlands 179/2(2011-05-01), 277-286 0014-2336 179:2<277 2011 179 10681 https://doi.org/10.1007/s10681-010-0308-7 text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1007/s10681-010-0308-7 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Ignacimuthu S. Plant Biotechnology Unit, Entomology Research Institute, Loyola College, 600 034, Chennai, Tamil Nadu, India aut NATIONALLICENCE 700 1- Raveendar S. Plant Biotechnology Unit, Entomology Research Institute, Loyola College, 600 034, Chennai, Tamil Nadu, India aut NATIONALLICENCE 773 0- Euphytica Springer Netherlands 179/2(2011-05-01), 277-286 0014-2336 179:2<277 2011 179 10681 Metadata rights reserved Springer special CC-BY-NC licence nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer