caa a22 4500 445323361 CHVBK 20180317142711.0 cr unu---uuuuu 170323e20110101xx s 000 0 eng 10.1007/s10681-010-0243-7 doi (NATIONALLICENCE)springer-10.1007/s10681-010-0243-7 Impact of grafting on cold responsive gene expression in Satsuma mandarin ( Citrus unshiu ) [Elektronische Daten] [Ying Huang, Ying Si, Fenny Dane] The major limitation preventing the expansion of Satsuma mandarin (Citrus unshiu) production in the Gulf Coast regions of the US is the risk of freeze damage. Poncirus trifoliata (L.) Raf has been widely used as a cold hardy rootstock for citrus production in freeze-prone environments. In this study, relative quantitative real-time RT-PCR was used to study cold responsive genes in Satsuma mandarin (C. unshiu), C. unshiu grafted on P. trifoliata rootstock and P. trifoliata following exposure to cold shock or cold acclimation treatments. Major changes in gene expression occurred in the different species during cold stress especially with respect to nitrate transporter, auxin responsive GH3, UDP-glycosyltransferase (UGT) and chalcone-flavanone isomerase. Very high levels of nitrate transporter were reached early in Poncirus following 60h of cold shock treatment at 4°C, and in C. unshiu after 3weeks of gradual cold acclimation. Also, high levels of auxin responsive GH3 were reached in Poncirus following both cold shock and cold acclimation, while levels in C. unshiu were low or reduced. Poncirus rootstock significantly affected gene expression in C. unshiu scion, resulting in a reduction of nitrate transporter, UGT and trehalose 6-phosphate synthase expression levels, and an increase in auxin responsive GH3 gene during cold acclimation. The impact of rootstock on citrus scion during cold stress might be associated with partitioning of nutrients and hormones. Springer Science+Business Media B.V., 2010 Cold stress nationallicence Poncirus trifoliata nationallicence Citrus unshiu nationallicence Grafting nationallicence Quantitative real-time RT-PCR nationallicence Gene expression nationallicence CBF : C-repeat binding factor nationallicence CHI : Chalcone-flavanone isomerase nationallicence COR : Cold responsive nationallicence DREB : Dehydration-responsive element-binding factor nationallicence IAA : Indole acetic acid nationallicence MAPK : Mitogen activated protein kinase nationallicence RT-PCR : Reverse transcriptase polymerase chain reaction nationallicence RQ : Relative quantitative nationallicence TPS : Trehalose 6-phosphate synthase nationallicence UGT : UDP-glycosyltransferase nationallicence Huang Ying Department of Horticulture, Auburn University, 36849, Auburn, AL, USA aut Si Ying Department of Horticulture, Auburn University, 36849, Auburn, AL, USA aut Dane Fenny Department of Horticulture, Auburn University, 36849, Auburn, AL, USA aut Euphytica Springer Netherlands 177/1(2011-01-01), 25-32 0014-2336 177:1<25 2011 177 10681 https://doi.org/10.1007/s10681-010-0243-7 text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1007/s10681-010-0243-7 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Huang Ying Department of Horticulture, Auburn University, 36849, Auburn, AL, USA aut NATIONALLICENCE 700 1- Si Ying Department of Horticulture, Auburn University, 36849, Auburn, AL, USA aut NATIONALLICENCE 700 1- Dane Fenny Department of Horticulture, Auburn University, 36849, Auburn, AL, USA aut NATIONALLICENCE 773 0- Euphytica Springer Netherlands 177/1(2011-01-01), 25-32 0014-2336 177:1<25 2011 177 10681 Metadata rights reserved Springer special CC-BY-NC licence nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer