caa a22 4500 44532337X CHVBK 20180317142711.0 cr unu---uuuuu 170323e20110101xx s 000 0 eng 10.1007/s10681-010-0295-8 doi (NATIONALLICENCE)springer-10.1007/s10681-010-0295-8 A practical method for apple cultivar identification and parent-offspring analysis using simple sequence repeat markers [Elektronische Daten] [Shigeki Moriya, Hiroshi Iwanami, Kazuma Okada, Toshiya Yamamoto, Kazuyuki Abe] Differentiation of cultivars with simple sequence repeat (SSR) markers is a very useful technique for the true-to-type characterization of cultivars and clarification of parent-offspring relationships. We developed an SSR marker set for cultivar identification comprising 15 markers that were screened from 46 previously published SSRs. This marker set could be used for apple varieties including Malus×domestica and/or other Malus species. These SSRs successfully characterized 95 apples, including the leading and major founding cultivars used worldwide for modern apple breeding. Therefore, this marker set could be applied to almost all apple cultivars. We also analyzed the parent-offspring relationships of 69 cultivars by considering allele transmissions. This analysis revealed the true parentage of the following seven cultivars: ‘Kizashi', ‘Chinatsu', ‘Honey Queen', ‘Haruka', ‘Seirin', ‘Ozenokurenai', and Morioka #48. This analysis also revealed a parentage discrepancy for ‘Hacnine'. From the parent-offspring analysis, two microsatellite mutation events at alleles inherited from pollen parents were observed. Springer Science+Business Media B.V., 2010 Cultivar identification nationallicence Malus spp nationallicence Mutation nationallicence Simple sequence repeat nationallicence Moriya Shigeki Apple Breeding and Physiology Research Team, National Institute of Fruit Tree Science, National Agriculture and Food Research Organization (NARO), Shimokuriyagawa, Morioka, 020-0123, Iwate, Japan aut Iwanami Hiroshi Apple Breeding and Physiology Research Team, National Institute of Fruit Tree Science, National Agriculture and Food Research Organization (NARO), Shimokuriyagawa, Morioka, 020-0123, Iwate, Japan aut Okada Kazuma Apple Breeding and Physiology Research Team, National Institute of Fruit Tree Science, National Agriculture and Food Research Organization (NARO), Shimokuriyagawa, Morioka, 020-0123, Iwate, Japan aut Yamamoto Toshiya Fruit Genome Research Team, National Institute of Fruit Tree Science, National Agriculture and Food Research Organization (NARO), Fujimoto, Tsukuba, 305-8605, Ibaraki, Japan aut Abe Kazuyuki Apple Breeding and Physiology Research Team, National Institute of Fruit Tree Science, National Agriculture and Food Research Organization (NARO), Shimokuriyagawa, Morioka, 020-0123, Iwate, Japan aut Euphytica Springer Netherlands 177/1(2011-01-01), 135-150 0014-2336 177:1<135 2011 177 10681 https://doi.org/10.1007/s10681-010-0295-8 text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1007/s10681-010-0295-8 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Moriya Shigeki Apple Breeding and Physiology Research Team, National Institute of Fruit Tree Science, National Agriculture and Food Research Organization (NARO), Shimokuriyagawa, Morioka, 020-0123, Iwate, Japan aut NATIONALLICENCE 700 1- Iwanami Hiroshi Apple Breeding and Physiology Research Team, National Institute of Fruit Tree Science, National Agriculture and Food Research Organization (NARO), Shimokuriyagawa, Morioka, 020-0123, Iwate, Japan aut NATIONALLICENCE 700 1- Okada Kazuma Apple Breeding and Physiology Research Team, National Institute of Fruit Tree Science, National Agriculture and Food Research Organization (NARO), Shimokuriyagawa, Morioka, 020-0123, Iwate, Japan aut NATIONALLICENCE 700 1- Yamamoto Toshiya Fruit Genome Research Team, National Institute of Fruit Tree Science, National Agriculture and Food Research Organization (NARO), Fujimoto, Tsukuba, 305-8605, Ibaraki, Japan aut NATIONALLICENCE 700 1- Abe Kazuyuki Apple Breeding and Physiology Research Team, National Institute of Fruit Tree Science, National Agriculture and Food Research Organization (NARO), Shimokuriyagawa, Morioka, 020-0123, Iwate, Japan aut NATIONALLICENCE 773 0- Euphytica Springer Netherlands 177/1(2011-01-01), 135-150 0014-2336 177:1<135 2011 177 10681 Metadata rights reserved Springer special CC-BY-NC licence nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer