caa a22 4500 445323388 CHVBK 20180317142711.0 cr unu---uuuuu 170323e20110101xx s 000 0 eng 10.1007/s10681-010-0259-z doi (NATIONALLICENCE)springer-10.1007/s10681-010-0259-z Effect of DNA methylation on molecular diversity of watermelon heirlooms and stability of methylation specific polymorphisms across the genealogies [Elektronische Daten] [Padma Nimmakayala, Gopinath Vajja, Renée Gist, Yan Tomason, Amnon Levi, Umesh Reddy] American watermelon heirlooms are phenotypically diverse in terms of their growth habits, fruit traits and responses to biotic and abiotic stress. Wide ranging DNA marker tools resolved narrow molecular diversity among these collections. The current research explored additional insights such as extent of diversity at the methylation level among the watermelon cultivars. DNA profiles were generated using Methylation-sensitive AFLP assay for 47 watermelon heirlooms. Results indicated that methylation specific diversity (43%) in US watermelon heirlooms is higher than the diversity (19.8%) estimated by several investigators using conventional DNA markers. In tree topologies of Neighbor-Joining (NJ) phenograms, the clustering pattern of principal component analyses of separate data sets obtained from the methylation specific isoschizomers MspI and HpaII resolved the diversity associated with methylation. Methylation-induced clustering was further verified using model-based population structure analysis. Our study clearly revealed the extent of methylations that are shared between parental heirlooms and progeny heirlooms, when tracked in known genealogies and breeding histories of heirlooms. Methylation sites that were not carried over and de novo methylations in the progeny heirlooms were fewer, when compared to the methylations that are stable. Springer Science+Business Media B.V., 2010 mAFLP nationallicence Watermelon heirlooms nationallicence Methylation nationallicence Epigenetic diversity nationallicence Linkage disequilibrium nationallicence Nimmakayala Padma Gus R. Douglass Institute and Department of Biology, West Virginia State University, 25112-1000, Institute, WV, USA aut Vajja Gopinath Gus R. Douglass Institute and Department of Biology, West Virginia State University, 25112-1000, Institute, WV, USA aut Gist Renée Gus R. Douglass Institute and Department of Biology, West Virginia State University, 25112-1000, Institute, WV, USA aut Tomason Yan Gus R. Douglass Institute and Department of Biology, West Virginia State University, 25112-1000, Institute, WV, USA aut Levi Amnon US Vegetable Laboratory, USDA, ARS, 2875 Savannah Highway, 29414, Charleston, SC, USA aut Reddy Umesh Gus R. Douglass Institute and Department of Biology, West Virginia State University, 25112-1000, Institute, WV, USA aut Euphytica Springer Netherlands 177/1(2011-01-01), 79-89 0014-2336 177:1<79 2011 177 10681 https://doi.org/10.1007/s10681-010-0259-z text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1007/s10681-010-0259-z text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Nimmakayala Padma Gus R. Douglass Institute and Department of Biology, West Virginia State University, 25112-1000, Institute, WV, USA aut NATIONALLICENCE 700 1- Vajja Gopinath Gus R. Douglass Institute and Department of Biology, West Virginia State University, 25112-1000, Institute, WV, USA aut NATIONALLICENCE 700 1- Gist Renée Gus R. Douglass Institute and Department of Biology, West Virginia State University, 25112-1000, Institute, WV, USA aut NATIONALLICENCE 700 1- Tomason Yan Gus R. Douglass Institute and Department of Biology, West Virginia State University, 25112-1000, Institute, WV, USA aut NATIONALLICENCE 700 1- Levi Amnon US Vegetable Laboratory, USDA, ARS, 2875 Savannah Highway, 29414, Charleston, SC, USA aut NATIONALLICENCE 700 1- Reddy Umesh Gus R. Douglass Institute and Department of Biology, West Virginia State University, 25112-1000, Institute, WV, USA aut NATIONALLICENCE 773 0- Euphytica Springer Netherlands 177/1(2011-01-01), 79-89 0014-2336 177:1<79 2011 177 10681 Metadata rights reserved Springer special CC-BY-NC licence nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer