caa a22 4500 445323396 CHVBK 20180317142711.0 cr unu---uuuuu 170323e20110101xx s 000 0 eng 10.1007/s10681-010-0258-0 doi (NATIONALLICENCE)springer-10.1007/s10681-010-0258-0 Molecular and phenotypic characterization of the S -locus and determination of flowering time in new ‘Marcona' and ‘Desmayo Largueta'-type almond ( Prunus dulcis ) selections [Elektronische Daten] [Pedro Martínez-García, Fernando Mañas, Prudencio López, Federico Dicenta, Encarnación Ortega] ‘Marcona' and ‘Desmayo Largueta' are the most widely cultivated almonds in Spain, representing around 27% of the total production and 34% of the entire almond growing surface. The excellent quality of their kernels makes them highly appreciated and demanded worldwide. However, due to their self-incompatibility, they should be grown along with cross-compatible cultivars, whose lower kernel quality often reduces the economic benefits of the plantation. In addition, although they are cross-compatible, are not good candidates to share the orchard since ‘Desmayo Largueta' usually flowers earlier than ‘Marcona'. Therefore, to optimize orchard yield, genotypes with overlapping flowering times, cross-compatible with these cultivars and of similar fruit and ripening characteristics are desirable. In order to find suitable pollinators of these cultivars, five ‘Marcona' and four ‘Desmayo Largueta'-type selections from "Instituto Técnico Agronómico Provincial (ITAP) de Albacete” (Spain) were characterized for flowering time and for self and cross-incompatibility. The results obtained showed that the nine ITAP selections were self-incompatible, and that three and one were promising candidates as pollinators of ‘Marcona' and ‘Desmayo Largueta', respectively. The S-haplotypes of two ITAP selections were characterized by cloning and sequencing their pistil S (S-RNase) and pollen S (SFB) genes. The results also showed that S f RNase does not have a mutated histidine in C2 region, and detected differences with other previously published sequences for S 23 RNase and SFB 23 allele. Moreover, the sequence for almond SFB 27 allele is reported here for the first time. Springer Science+Business Media B.V., 2010 Prunus dulcis nationallicence Flowering time nationallicence Self-incompatibility nationallicence Cross-compatibility nationallicence S -haplotype characterization nationallicence Martínez-García Pedro Dpto. de Mejora Vegetal, CEBAS-CSIC, Campus Universitario de Espinardo, P.O. Box 164, 30100, Murcia, Spain aut Mañas Fernando Instituto Técnico Agronómico Provincial de Albacete (ITAP), Avda. Gregorio Arcos s/n, P.O. Box 451, 02080, Albacete, Spain aut López Prudencio Instituto Técnico Agronómico Provincial de Albacete (ITAP), Avda. Gregorio Arcos s/n, P.O. Box 451, 02080, Albacete, Spain aut Dicenta Federico Dpto. de Mejora Vegetal, CEBAS-CSIC, Campus Universitario de Espinardo, P.O. Box 164, 30100, Murcia, Spain aut Ortega Encarnación Dpto. de Mejora Vegetal, CEBAS-CSIC, Campus Universitario de Espinardo, P.O. Box 164, 30100, Murcia, Spain aut Euphytica Springer Netherlands 177/1(2011-01-01), 67-78 0014-2336 177:1<67 2011 177 10681 https://doi.org/10.1007/s10681-010-0258-0 text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1007/s10681-010-0258-0 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Martínez-García Pedro Dpto. de Mejora Vegetal, CEBAS-CSIC, Campus Universitario de Espinardo, P.O. Box 164, 30100, Murcia, Spain aut NATIONALLICENCE 700 1- Mañas Fernando Instituto Técnico Agronómico Provincial de Albacete (ITAP), Avda. Gregorio Arcos s/n, P.O. Box 451, 02080, Albacete, Spain aut NATIONALLICENCE 700 1- López Prudencio Instituto Técnico Agronómico Provincial de Albacete (ITAP), Avda. Gregorio Arcos s/n, P.O. Box 451, 02080, Albacete, Spain aut NATIONALLICENCE 700 1- Dicenta Federico Dpto. de Mejora Vegetal, CEBAS-CSIC, Campus Universitario de Espinardo, P.O. Box 164, 30100, Murcia, Spain aut NATIONALLICENCE 700 1- Ortega Encarnación Dpto. de Mejora Vegetal, CEBAS-CSIC, Campus Universitario de Espinardo, P.O. Box 164, 30100, Murcia, Spain aut NATIONALLICENCE 773 0- Euphytica Springer Netherlands 177/1(2011-01-01), 67-78 0014-2336 177:1<67 2011 177 10681 Metadata rights reserved Springer special CC-BY-NC licence nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer