caa a22 4500 445323418 CHVBK 20180317142711.0 cr unu---uuuuu 170323e20110101xx s 000 0 eng 10.1007/s10681-010-0265-1 doi (NATIONALLICENCE)springer-10.1007/s10681-010-0265-1 Sex distinction of asparagus by loop-mediated isothermal amplification and observation of seedling phenotypes [Elektronische Daten] [Yukie Shiobara, Mioko Yoshino, Atsuko Uragami, Ani Widiastuti, Akifumi Omori, Keiko Kuba, Harufumi Saito, Yukimasa Hirata, Takahiro Sonoda, Takeharu Koizumi, Tatsuo Sato] Asparagus (Asparagus officinalis L.) is a dioecious plant. In general, male and female plants are used for open-field culture and intensive cultivation, respectively. Farmers distinguish between the sexes by observing the form of the flower organs. However, because flowering begins 2-3years after planting, the sexes cannot be differentiated at transplantation by using this method, and planting of an all-male population is not possible. In this study, the usefulness of loop-mediated isothermal amplification (LAMP), a simple method of gene amplification, for sex distinction at the DNA level was determined. In addition, the phenotypic differences in seeds and seedlings of male and female plants were investigated for application as a method of early sex distinction. By using the LAMP method, the sex could be correctly identified in 100% of the seedlings, suggesting that this method is effective for sex distinction at the gene level. Principal component analysis was conducted with 11 selected parameters after investigating the seeds and seedlings of both male and female plants. The results revealed that male plants tend to have many stalks or cladophylls and female plants tend to have large plant forms, suggesting that the sexes can be distinguished by the external appearance of the seedlings before planting. LAMP and observation of the seedling phenotypes could be useful methods of sex distinction for increasing the efficiency of asparagus breeding. Springer Science+Business Media B.V., 2010 External appearance nationallicence Molecular marker nationallicence Sex distinction nationallicence Sex-specific character nationallicence Shiobara Yukie Field Science Center, College of Agriculture, Ibaraki University, Ami 4668-1, Ami, 300-0331, Inashiki, Ibaraki, Japan aut Yoshino Mioko Field Science Center, College of Agriculture, Ibaraki University, Ami 4668-1, Ami, 300-0331, Inashiki, Ibaraki, Japan aut Uragami Atsuko National Institute of Vegetable and Tea Science, 305-8666, Tsukuba, Ibaraki, Japan aut Widiastuti Ani United Graduate School of Agricultural Science, Tokyo University of Agriculture and Technology, 183-8509, Fuchu, Tokyo, Japan aut Omori Akifumi Field Science Center, College of Agriculture, Ibaraki University, Ami 4668-1, Ami, 300-0331, Inashiki, Ibaraki, Japan aut Kuba Keiko Field Science Center, College of Agriculture, Ibaraki University, Ami 4668-1, Ami, 300-0331, Inashiki, Ibaraki, Japan aut Saito Harufumi Field Science Center, College of Agriculture, Ibaraki University, Ami 4668-1, Ami, 300-0331, Inashiki, Ibaraki, Japan aut Hirata Yukimasa Plant Bio Center, Wakayama Federation of Agricultural Cooperatives, Misono, 640-8331, Wakayama, Wakayama, Japan aut Sonoda Takahiro Fukushima Prefectural Government, Sugitsuma, 960-8670, Fukushima, Fukushima, Japan aut Koizumi Takeharu Gunma Agricultural Research Center, Nishi-obokatamachi, 379-2224, Isesaki, Gunma, Japan aut Sato Tatsuo Field Science Center, College of Agriculture, Ibaraki University, Ami 4668-1, Ami, 300-0331, Inashiki, Ibaraki, Japan aut Euphytica Springer Netherlands 177/1(2011-01-01), 91-97 0014-2336 177:1<91 2011 177 10681 https://doi.org/10.1007/s10681-010-0265-1 text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1007/s10681-010-0265-1 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Shiobara Yukie Field Science Center, College of Agriculture, Ibaraki University, Ami 4668-1, Ami, 300-0331, Inashiki, Ibaraki, Japan aut NATIONALLICENCE 700 1- Yoshino Mioko Field Science Center, College of Agriculture, Ibaraki University, Ami 4668-1, Ami, 300-0331, Inashiki, Ibaraki, Japan aut NATIONALLICENCE 700 1- Uragami Atsuko National Institute of Vegetable and Tea Science, 305-8666, Tsukuba, Ibaraki, Japan aut NATIONALLICENCE 700 1- Widiastuti Ani United Graduate School of Agricultural Science, Tokyo University of Agriculture and Technology, 183-8509, Fuchu, Tokyo, Japan aut NATIONALLICENCE 700 1- Omori Akifumi Field Science Center, College of Agriculture, Ibaraki University, Ami 4668-1, Ami, 300-0331, Inashiki, Ibaraki, Japan aut NATIONALLICENCE 700 1- Kuba Keiko Field Science Center, College of Agriculture, Ibaraki University, Ami 4668-1, Ami, 300-0331, Inashiki, Ibaraki, Japan aut NATIONALLICENCE 700 1- Saito Harufumi Field Science Center, College of Agriculture, Ibaraki University, Ami 4668-1, Ami, 300-0331, Inashiki, Ibaraki, Japan aut NATIONALLICENCE 700 1- Hirata Yukimasa Plant Bio Center, Wakayama Federation of Agricultural Cooperatives, Misono, 640-8331, Wakayama, Wakayama, Japan aut NATIONALLICENCE 700 1- Sonoda Takahiro Fukushima Prefectural Government, Sugitsuma, 960-8670, Fukushima, Fukushima, Japan aut NATIONALLICENCE 700 1- Koizumi Takeharu Gunma Agricultural Research Center, Nishi-obokatamachi, 379-2224, Isesaki, Gunma, Japan aut NATIONALLICENCE 700 1- Sato Tatsuo Field Science Center, College of Agriculture, Ibaraki University, Ami 4668-1, Ami, 300-0331, Inashiki, Ibaraki, Japan aut NATIONALLICENCE 773 0- Euphytica Springer Netherlands 177/1(2011-01-01), 91-97 0014-2336 177:1<91 2011 177 10681 Metadata rights reserved Springer special CC-BY-NC licence nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer