caa a22 4500 445323434 CHVBK 20180317142712.0 cr unu---uuuuu 170323e20110101xx s 000 0 eng 10.1007/s10681-010-0275-z doi (NATIONALLICENCE)springer-10.1007/s10681-010-0275-z Wx intron variations support an allohexaploid origin of the sweetpotato [ Ipomoea batatas (L.) Lam] [Elektronische Daten] [Ming Gao, Glory Ashu, Lakeisha Stewart, William Akwe, Victor Njiti, Sandra Barnes] To clarify the polyploid origin of the sweetpotato, we analyzed retentions of three distinctive types of Waxy intron 2 (Wx-In2) variants among 27 sweetpotato lines and 24 selected relatives and their phylogenetic relationships with Wx-In2 from 11 closest relatives. The three types of Wx-In2 effectively distinguish three diploid constituent genomes of very close homeology in the sweetpotato: Type I is characteristic of some loci in Genome I and III, and Types II and III are specific to loci in Genome II and III. The Type I Wx-In2 variation was found to be retained in 19 sweetpotato lines, Ipomoea littoralis Blume (4×), I. tabascana (4×), and I. tenuissima (2×); Type II to be retained in all 27 sweetpotato lines, I. littoralis Blume, and two I. trifida accessions; Type III to be retained in 13 sweetpotato lines, I. tenuissima, and four distantly related species. Because of the nature of independent random divergence of orthologous intronic sequences, these highly selective retentions of genome-specific or characteristic sweetpotato Wx-In2 variations among four diploid or tetraploid sweetpotato relatives are consistent only with separate lineages of diploid genomes of the sweetpotato. Such an allohexaploid origin of the sweetpotato probably occurred via hybridization between I. tenuissima and I. littoralis Blume, derived earlier from I. trifida and an unidentified species sibling to I. tenuissima. However, neither the involvement of I. tabascana nor a multiple origin of the sweetpotato can be ruled out. The inference is supported by maximal likelihood relationships between the three types of Wx-In2 from the sweetpotato and Wx-In2 from its 11 closest relatives. Springer Science+Business Media B.V., 2010 Allohexaploid origin nationallicence Ipomoea littoralis nationallicence I. tenuissima nationallicence I. trifida nationallicence Sweetpotato progenitors nationallicence Waxy nationallicence Wx intron 2 variations nationallicence Gao Ming Center for Biotechnology and Genomics, Alcorn State University, 1000 ASU Drive, #682, 39096, Alcorn State, MS, USA aut Ashu Glory Center for Biotechnology and Genomics, Alcorn State University, 1000 ASU Drive, #682, 39096, Alcorn State, MS, USA aut Stewart Lakeisha Center for Biotechnology and Genomics, Alcorn State University, 1000 ASU Drive, #682, 39096, Alcorn State, MS, USA aut Akwe William Center for Biotechnology and Genomics, Alcorn State University, 1000 ASU Drive, #682, 39096, Alcorn State, MS, USA aut Njiti Victor Center for Biotechnology and Genomics, Alcorn State University, 1000 ASU Drive, #682, 39096, Alcorn State, MS, USA aut Barnes Sandra Center for Biotechnology and Genomics, Alcorn State University, 1000 ASU Drive, #682, 39096, Alcorn State, MS, USA aut Euphytica Springer Netherlands 177/1(2011-01-01), 111-133 0014-2336 177:1<111 2011 177 10681 https://doi.org/10.1007/s10681-010-0275-z text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1007/s10681-010-0275-z text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Gao Ming Center for Biotechnology and Genomics, Alcorn State University, 1000 ASU Drive, #682, 39096, Alcorn State, MS, USA aut NATIONALLICENCE 700 1- Ashu Glory Center for Biotechnology and Genomics, Alcorn State University, 1000 ASU Drive, #682, 39096, Alcorn State, MS, USA aut NATIONALLICENCE 700 1- Stewart Lakeisha Center for Biotechnology and Genomics, Alcorn State University, 1000 ASU Drive, #682, 39096, Alcorn State, MS, USA aut NATIONALLICENCE 700 1- Akwe William Center for Biotechnology and Genomics, Alcorn State University, 1000 ASU Drive, #682, 39096, Alcorn State, MS, USA aut NATIONALLICENCE 700 1- Njiti Victor Center for Biotechnology and Genomics, Alcorn State University, 1000 ASU Drive, #682, 39096, Alcorn State, MS, USA aut NATIONALLICENCE 700 1- Barnes Sandra Center for Biotechnology and Genomics, Alcorn State University, 1000 ASU Drive, #682, 39096, Alcorn State, MS, USA aut NATIONALLICENCE 773 0- Euphytica Springer Netherlands 177/1(2011-01-01), 111-133 0014-2336 177:1<111 2011 177 10681 Metadata rights reserved Springer special CC-BY-NC licence nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer